NEW DIFFUSE REFLECTANCE INFRARED FOURIER TRANSFORM SPECTROSCOPY FOR THE ESTIMATION OF TRAMADOL HYDROCHLORIDE CAPSULES

VENKATA SAI GANESHA PRAVALLIKA BURELA; USHA RANI NAMMI; PRANATI MANDALEMULA

doi:10.22159/ijpps.2023v15i1.46464

Authors

VENKATA SAI GANESHA PRAVALLIKA BURELA Maharajah’s College of Pharmacy, Vizianagaram, India https://orcid.org/0000-0002-1403-2906
USHA RANI NAMMI Maharajah’s College of Pharmacy, Vizianagaram, India
PRANATI MANDALEMULA Maharajah’s College of Pharmacy, Vizianagaram, India

DOI:

https://doi.org/10.22159/ijpps.2023v15i1.46464

Keywords:

Tramadol hydrochloride, Diffuse reflectance infrared fourier transform spectroscopy (DRIFTS), Validation, Quantification

Abstract

Objective: The objective of the study was to develop and validate a new diffuse reflectance infrared fourier transform (DRIFT) spectroscopic method for the quantification of Tramadol HCl in the pharmaceutical dosage form.

Methods: The Fourier Transform Infrared (FTIR) analysis was carried out on Cary 630 FTIR spectrophotometer (Agilent Technologies, USA) equipped with a diffuse reflectance sampling interface (650-4000 cm^-1, 32 scans, 8 cm^-1 resolution). The solid-state samples were prepared by dilution in dry potassium bromide and were analysed by an FTIR spectrophotometer with the DRIFT sampling technique, which follows the Kubelka-Munk model depicting the theory of diffuse reflectance at scattering surfaces, relating band intensities to concentration for transmission measurements similar to Beer’s law.

Results: A linear relationship was found in the selected wavenumber range of 3295-3298 cm^-1denoting the carboxyl peak in the concentration range of 0.6-3.0% w/w with a good correlation coefficient of 0.997. The percent recovery of Tramadol HCl in the marketed dosage form was found to be 100.4%.

Conclusion: The proposed method was found to be accurate, precise, reproducible, and eco-friendly. DRIFT spectroscopy may have the potential as an alternative method for qualitative and quantitative analysis of Tramadol HCl in its capsules.

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