SUB-LETHAL TOXICITY OF POTASSIUM CYANIDE ON NILE TILAPIA (OREOCHROMIS NILOTICUS): BIOCHEMICAL RESPONSE
Keywords:
Nile tilapia, Oreochromis niloticus, Cyanide toxicity, Serum biochemical profileAbstract
Objective: The present study was carried out to investigate the influences of sublethal toxicity of potassium cyanide (KCN) exposure to Oreochromis niloticus with special reference to blood biochemistry.
Methods: Three groups of fish (25 in each group) were treated with different concentrations of 0 (control), 0.1 and 0.2 mg/L KCN for 2 weeks. The Blood samples were drawn after 2 weeks of exposure and serum biochemical analysis including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), glucose, triglyceride, cholesterol, creatinine, total protein, and albumin activities were measured.
Results: KCN exposure at both concentrations caused significant (P<0.05) elevation of AST and LDH activities and creatinine concentration as compared to the control. Indeed, ALT, ALP and glucose levels in the fish treated with 0.2 mg/L KCN were significantly higher than those levels from controls. On the other hand, the biochemical parameters including GGT, triglyceride, cholesterol, total protein, and albumin did not change significantly following cyanide exposure.
Conclusion: The current study has clearly indicated the alterations in some of the measured serum biochemical parameters which reflect tissue damages, especially in liver and kidney, due to chronic cyanide intoxication in Oreochromis niloticus and may be used for better understanding the pathophysiology of this toxicity and as an aid in the diagnosis of cyanide poisoning in fishes.
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Prashanth MS, Neelagund SE. Free cyanide-induced Biochemical changes in Nitrogen metabolism of the Indian major carp, Cirrhinus mrigala. J Basic Clin Physiol Pharmacol 2007;8(4):77-287.
Okolie NP, Audu K. Correlation between cyanideinduced decreases in ocular Ca2+-ATPase and lenticular opacification. J Biomed Sci 2004;3(1):37-4.
Daya S, Walker RB, Anoopkumar-Dukie S. Cyanide-induced free radical production and lipid peroxidation in rat brain homogenate is reduced by aspirin. Metab Brain Dis 2000;15(3):203-10.
Okolie NP, Osagie AU. Liver and kidney lesions and associated enzyme changes induced in rabbits by chronic cyanide exposure. Food Chem Toxicol 1999;37:745-50.
Dube PN, Shwetha A, Hosetti BB. Effect of exposure to sublethal concentrations of sodium cyanide on the carbohydrate metabolism of the Indian Major Carp Labeo rohita (Hamilton, 1822). Pesqui Vet Bras 2013;33(7):914-9.
Shwetha A, Hosetti BB. Acute effects of zinc cyanide on the behaviour and oxygen consumption of the Indian major carp, Cirrhinus mrigala. World J Zoology 2009;4(3):238-46.
Agency for toxic substances and disease registry (ATSDR), Toxicological profile for cyanide. Draft for public comment. Prepared for the U. S. Public Health Service by Technical Resources, Inc., under Contract No. 68-03-3268. Revised by Syracuse Research Corporation under Contract No. 68-03-3521. Oak Ridge National Laboratory; 1988.
US. Environmental protection agency. Cyanide health advisory (draft). Office of Drinking Water; 1985.
World Health Organization, Cyanide. In: Guidelines for drinking-water quality. Vol. 2. Health criteria and other supporting information. Geneva. 1984. p. 97.
Flynn CM, Haslem SM. Cyanide chemistry-precious metals processing and waste treatment: US. Bur Mines Inf Circ 1995;9429:282.
Eisler R. Cyanide Hazards to Fish, Wildlife, and Invertebrate: A Synoptic Review: Contaminant Hazard Review report 23, US. Dept. Interior, Fish and Wildlife Service; 1991. p. 55.
Heming T, Thurston RV, Meyn EL, Zajdel R. Acute toxicity of thiocyanate to trout. Trans Am Fish Soc 1985;114:895-905.
Tsadik GG, Bart AN. Effects of feeding, stocking density and water-flow rate on fecundity, spawning frequency and egg quality of Nile tilapia, Oreochromis niloticus (L.). Aquacult 2007;272:380-8.
Ridha MT. Comparative study of growth performance of three strains of Nile tilapia, Oreochromis niloticus, L. at two stocking densities. Aquacult Res 2006;37:172-9.
Yue YR, Zhou QC. Effect of replacing soybean meal with cottonseed meal on growth, feed utilization and hematological indexes for juvenile hybrid tilapia, Oreochromis niloticus x O. aureus. Aquacult 2008;284:185-9.
Ballantyne B. Toxicology of cyanides. In: Clinical and experimental toxicology of cyanides. B Ballantyne, TC Marrs, Eds. IOP Pub. Ltd, Bristol; 1987. p. 41-126.
Okolie NP, Osagie AU. Differential effects of chronic cyanide intoxication on heart, lung and pancreatic tissues. Food Chem Toxicol 2000;38(6):543-8.
Soto-Blanco B, Marioka PC, Gorniak SL. Effects of long-term low-dose cyanide administration to rats. Ecotoxicol Environ Saf 2002;53(1):37-41.
Okolie NP, Iroanya CU. Some histologic and biochemical evidence for mitigation of cyanide-induced tissue lesions by antioxidant vitamin administration in rabbits. Food Chem Toxicol 2003;41:463-9.
Soto-Blanco B, Gorniak SL. Milk transfer of cyanide and thiocyanate: cyanide exposure by lactation in goats. Vet Res 2003;34:213-20.
Manzano H, Sousa AB, Soto-Blanco B, Guerra JL, Maiorka PC, Gorniak SL. Effects of long-term cyanide ingestion by pigs. Vet Res Commun 2007;31:93-104.
Saksena DK. Biomonitoring of Pb, Ni, Cr, Hg with the help of bryophytes in Nainital. In: V Nath, Bishen Singh, Mahenra Pal Singh, Ed. Proceeding of National Conference on Bryology; 1999. p. 159-76.
Davis JRE. Molecular and cell biology. In: Mechanisms of disease, Tomlinson S. Heagerty AM, Weetman AP, eds. Cambridge University Press: Cambridge, UK; 1997. p. 13-62.
Scott GR, Sloman KA. The effects of environmental pollutants on complex fish behaviour: integrative behavioural and physiological indicators of toxicity. Aquat Toxicol 2004;68:369-92.
Schuett DA, Lehmann J, Goerlich R, Hamers R. Hematology of swordtail, Xiphiphorus helleri. 1: Blood parameters and light microscopy of blood cells. J Appl Ichthyol 1997;13(2):83-9.
Hosetti BB, Dube PN. Evaluation of acute toxicity of copper cyanide to freshwater fish, Catla catla (Hamilton). J Central Eur Agric 2010;12(1):135-44.
Adeyemo OK. Haematological and histopathological effects of Cassava Mill Effluent in Clarias gariepinus. Afr J Biomed Res 2005;8(3):179-83.
Osman AGM, Abdel Reheem ABM, Abuelfadl KY, Gadelrab AG. Enzymatic and histopathologic biomarkers as indicators of aquatic pollution in fishes. Nat Sci 2010;2(11):1302-11.
David M, Ramesh H, Patil VK, Marigoudar SR, Chebbi SG. Sodium cyanide-induced modulations in the activities of some oxidative enzymes and metabolites in the fingerlings of C. carpio (L). Toxicol Environ Chem 2010;92:1841-9.
Prashanth MS. Acute toxicity, behavioral and nitrogen metabolism changes of sodium cyanide affected on tissues of Tilapia mossambica (Perters). Drug Chem Toxicol 2012;35(2):178-83.
Shwetha A, Hosetti BB, Dube PN. Toxic effects of zinc cyanide on some protein metabolites in freshwater fish, Cirrhinus mrigala (Hamilton). Int J Environ Res 2012;6(3):769-78.
Okafor PN, Anoruo K, Bonire AO, Maduagwu EN. The role of low-protein and cassava-cyanide intake in the aetiology of tropical pancreatitis. Global J Pharmacol 2008;2(1):6-10.
Elsaid FG, Elkomy MM. Aqueous garlic extract and sodium thiosulphate as antidotes for cyanide intoxication in Albino rats. Res J Medicine Med Sci 2006;1(2):50-6.
Naveed A, Janaiah C, Venkateshwarlu P. The effects of lihocin toxicity on Protein metabolism of the fresh water edible fish, Channa punctatus (Bloch). Toxicol Environ Health Sci 2010;3(1):18-23.
Agrahari S, Pandey KC, Gopal K. Biochemical alteration induced by monocrotophos in the blood plasma of fish, Channa punctatus (Bloch). Pesticide Biochem Physiol 2007;88:268-72.
Tulsawani RK, Debnath M, Pant SC, Kumar O, Prakash AO, Vijayaraghavan R, et al. Effect of sub-acute oral cyanide administration in rats: Protective efficacy of alpha-ketoglutarate and sodium thiosulfate. Chem-Biol Interact 2005;156:1-12.
Sousa AB, Soto-Blanco B, Guerra JL, Kimura ET, Gorniak SL. Does prolonged oral exposure to cyanide promote hepatotoxicity and nephrotoxicity? Toxicol 2002;174:87-95.
Bogusz M. The usefulness of enzymatic tests in acute poisoning. Arch Toxicol 1975;34:159-67.
Neff JM. Use of biochemical measurements to detect pollutant mediated damage to fish. In: Aquatic toxicology and hazard assessment: seventh symposium. RD Cardwell, R Purdy, RC Bahner, Eds. ASTM STP 854, ASTM, Philadelphia; 1985. p. 155-83.
Abdel-Hameid NAH. A protective effect of calcium carbonate against arsenic toxicity of the Nile catfish, Clarias gariepinus. Turkish J Fisheries Aquatic Sci 2009;9:191-200.
Kamalaveni K, Gopal V, Sampson U, Aruna D. Recycling and utilization of metabolic wastes for energy production is an index of biochemical adaptation of fish under environmental pollution stress. Environmental Monitoring and Assessment 2003;86:255-64.
Shobha Rani A, Sudharsan R, Reddy TN, Reddy PUM, Raju TN. Alternations in the levels of dehydrogenases in a freshwater fish, Tilapia mossambica (Peters) exposed to arsenic toxicity. Indian J Environ Health 2000;42:130-3.
Way JL. Cyanide intoxication and its mechanism of antagonism. Ann Rev Pharmacol Toxicol 1984;24:451-81.
Kamalu BP. The effect of a nutritionally-balanced cassava (Manihot esculenta, Crantz) diet on endocrine function using the dog as a model.1. Pancreas. Br J Nutr 1991;65:365-72.
Geldof AA, Becking JL, de Vries CD. Histopathological changes in rat pancreas after fasting and cassava feeding. In Vivo 1992;6:545-51.
Akanji AO, Famuyiwa OO. The effects of chronic cassava consumption, cyanide intoxication and protein malnutrition on glucose tolerance in growing rats. Br J Nutr 1993;69:269-76.
Petersen JM. Tropical pancreatitis. J Clin Gastroenterol 2002;35:61-6.
Jackson LC. Behavioral effects of chronic sublethal dietary cyanide in an animal model: implications for humans consuming cassava (Manihot esculenta). Hum Biol 1988;60:597-614.