• HEMA J. N. Department of Biotechnology, M. S. Ramaiah Institute of Technology, Bangalore 560054
  • SHOBHA Department of Botany, University College, Mangaluru 575001, Karnataka, India
  • SHRUTHI S. D. Microbiology and Molecular Biology Lab, BioEdge Solutions, Bengaluru, Karnataka, India



Cellulose-degrading bacteria, Cellulase, Carboxymethyl cellulose, Green plant waste, 16s rRNA


Objective: The green plant waste consists of a high amount of lignocellulosic materials offering an intense environment for the growth of cellulolytic bacteria, which have ability to degrade plant biomass as a carbon source. This cellulase produced can be used to break down plant waste into valuable products such as monomeric sugars, biofuels, compost etc. Therefore, the aim of present study was to isolate and identify potent cellulose-degrading bacteria from decomposing plant matter and assessment of their cellulolytic activity.

Methods: The cellulolytic bacteria were isolated by serial dilution technique on CMC agar media and six isolates were selected based on their cellulose hydrolysing ability. Based on the biochemical tests such as Oxidase test, Voges-Proskauer test, Methyl red test, Catalase test and sequence analysis of 16s rRNA genes.

Results: The isolates were identified as Paenibacillus alvei, Paenibacillus lentimorbus, Bacillus subtilis, Bacillus nakamurai with percentage identity of 93.77%, 92.80%, 97.71% and 91.94%, respectively, as obtained from NCBI BLAST. Among these Paenibacillus alvei showed the highest cellulase activity.

Conclusion: The findings of this study could pave the way for the use of cellulose as an inexpensive energy source for bacteria that are capable of producing valuable products.


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Sundarraj AA, Ranganathan TV. A review on cellulose and its utilization from agro-industrial waste. Drug Invent Today. 2018;10(1):89-94.

Lakhundi S, Siddiqui R, Khan NA. Cellulose degradation: a therapeutic strategy in the improved treatment of acanthamoeba infections. Parasit Vectors. 2015;8(1):23. doi: 10.1186/s13071-015-0642-7, PMID 25586209.

Hussain AA, Abdel Salam MS, Abo Ghalia HH, Hegazy WK, Hafez SS. Optimization and molecular identification of novel cellulose-degrading bacteria isolated from Egyptian environment. J Genet Eng Biotechnol. 2017;15(1):77-85. doi: 10.1016/j.jgeb.2017.02.007, PMID 30647644.

Kaur M. Isolation and screening of cellulose-degrading bacteria in kitchen waste and detecting their degrading potential. IOSRJMCE. 2012;1(2):33-5. doi: 10.9790/1684-0123335.

Zhang XZ, Zhang YHP. Cellulases: characteristics, sources, production, and applications. Bioprocess Technol Biorefin Sustain Prod Fuels Chem Polym. 2013;1:131-46.

Dar MA, Shaikh AA, Pawar KD, Pandit RS. Exploring the gut of Helicoverpa armigera for cellulose-degrading bacteria and evaluation of a potential strain for lignocellulosic biomass deconstruction. Process Biochem. 2018;73:142-53. doi: 10.1016/j.procbio.2018.08.001.

Jayasekara S, Ratnayake R. Microbial cellulases: an overview and applications. Cellulose. 2019;22.

Mahmood R, Afrin N, Jolly NS, Shilpi YR. Isolation and identification of cellulose-degrading bacteria from different types of samples. World J Environ Biosci. 2020;9(2):8-13.

Ejaz U, Sohail M, Ghanemi A. Cellulases: from bioactivity to a variety of industrial applications. Biomimetics (Basel). 2021;6(3):44. doi: 10.3390/biomimetics6030044, PMID 34287227.

Singh S, Jaiswal DK, Sivakumar N, Verma JP. Developing efficient thermophilic cellulose-degrading consortium for glucose production from different agro-residues. Front Energy Res. 2019;7:61. doi: 10.3389/fenrg.2019.00061.

Ahmad B, Nigar S, Shah SA, Bashir S, Ali J, Yousaf S. Isolation and identification of cellulose-degrading bacteria from municipal waste and their screening for potential antimicrobial activity. World Appl Sci J. 2013;27(11):1420-6.

Dubey SK, Meena RK, Sao S, Patel J, Thakur S, Shukla P. Isolation and characterization of cellulose-degrading bacteria from biogas slurry and their RAPD profiling. Curr Res Microbiol Biotechnol. 2014;2(4):416-21.

Mohammadipour Z, Enayatizamir N, Ghezelbash G, Moezzi A. Bacterial diversity and chemical properties of wheat straw-based compost leachate and screening of cellulase producing bacteria. Waste Biomass Valor. 2021;12(3):1293-302. doi: 10.1007/s12649-020-01119-w.

Dwarakanath PR, Rajakumari K, Thiruchelvi R. Isolation and characterization of glucanase producing bacteria from forest tree litter. Res J Pharm Technol. 2021;14(2):916-20. doi: 10.5958/0974-360X.2021.00163.3.

Rawway M, Ali SG, Badawy AS. Isolation and identification of cellulose-degrading bacteria from different sources at Assiut Governorate (Upper Egypt). J Eco Heal Env. 2018;6(1):15-24. doi: 10.18576/jehe/060103.

Xue C, Zhang Q, Owens G, Chen Z. A cellulose-degrading bacterial strain used to modify rice straw can enhance Cu(II) removal from aqueous solution. Chemosphere. 2020;256:127142. doi: 10.1016/j.chemosphere.2020.127142, PMID 32464362.

Liang YL, Zhang Z, Wu M, Wu Y, Feng JX. Isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of China and optimization of cellulase production by paenibacillus terrae ME27-1. BioMed Res Int. 2014;2014:512497. doi: 10.1155/2014/512497, PMID 25050355.

Manfredi AP, Perotti NI, Martinez MA. Cellulose-degrading bacteria isolated from industrial samples and the gut of native insects from Northwest of Argentina. J Basic Microbiol. 2015;55(12):1384-93. doi: 10.1002/jobm.201500269, PMID 26370071.

Jain D, Ravina AA, Bhojiya AA, Chauhan S, Rajpurohit D, Mohanty SR. Polyphasic characterization of plant growth promoting cellulose-degrading bacteria isolated from organic manures. Curr Microbiol. 2021;78(2):739-48. doi: 10.1007/s00284-020-02342-3, PMID 33416972.

Karim A, Nawaz MA, Aman A, Ul Qader SAU. Hyperproduction of cellulose-degrading endo (1,4) β-d-glucanase from Bacillus licheniformis KIBGE-IB2. J Radiat Res Appl Sci. 2015;8(2):160-5. doi: 10.1016/j.jrras.2014.06.004.

Sethi S, Datta A, Gupta BL, Gupta S. Optimization of cellulase production from bacteria isolated from soil. ISRN Biotechnol. 2013;2013:985685. doi: 10.5402/2013/985685, PMID 25937986.

Fathallh Eida MF, Nagaoka T, Wasaki J, Kouno K. Isolation and characterization of cellulose-decomposing bacteria inhabiting sawdust and coffee residue composts. Microbes Environ. 2012;27(3):226-33. doi: 10.1264/jsme2.me11299, PMID 22353767.

Kim YK, Lee SC, Cho YY, Oh HJ, Ko YH. Isolation of cellulolytic Bacillus subtilis strains from agricultural environments. ISRN Microbiol. 2012;2012:650563. doi: 10.5402/2012/650563, PMID 23724328.

Chien P, Yoo HS, Dykes G, Lee S. Isolation and characterization of cellulose-degrading ability in Paenibacillus isolates from landfill leachate. Malays J Microbiol. 2015;11(2):185-94.

Siu Rodas Y, Calixto Romo MLA, Guillen Navarro K, Sanchez JE, Zamora Briseno JA, Amaya Delgado L. Bacillus subtilis with endo cellulase and exocellulase activities isolated in the thermophilic phase from composting with coffee residues. Rev Argent Microbiol. 2018;50(3):234-43. doi: 10.1016/j.ram.2017.08.005, PMID 29289440.



How to Cite

J. N., H., SHOBHA, and S. S. D. “ISOLATION AND CHARACTERIZATION OF CELLULOSE-DEGRADING BACTERIA FROM DECOMPOSING PLANT MATTER”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 15, no. 4, Apr. 2023, pp. 22-27, doi:10.22159/ijpps.2023v15i4.47019.



Original Article(s)