ETHNOBOTANICAL SURVEY AND SCIENTIFIC VALIDATION OF MEDICINAL PLANTS USED IN THE TREATMENT OF FUNGAL INFECTIONS IN AGUMBE REGION OF WESTERN GHATS, INDIA
Keywords:
Antifungal, Western Ghats, Ethnobotanical, Combretum, Phytochemical, TanninAbstract
Objective: Western Ghats is one of the plant biodiversity hot spot of India. Agumbe region of Western Ghats is known for rich plant diversity and traditional medicinal practices. The aim of the study was to document ethnomedicinal practices followed in this region to treat fungal infections and their scientific validation in vitro.
Methods: An ethnobotanical survey was conducted to identify the plants used to treat fungal infections in Agumbe region of Western Ghats, India. Aqueous extracts of the plants selected based on the ethnobotanical survey were subjected to in vitro antifungal activity assay. Significantly active plant extract was subjected to activity guided separation methods. The active fraction was subjected to phytochemical, TLC bioautographic and IR spectral analysis to identify the active principle.
Results: Ten plants were identified to be used to treat fungal infections. Scientific validation by in vitro antifungal activity assay revealed significant inhibitory activity by aqueous extract of Combretum latifolium. Phytochemical and Infra red spectral analysis of the active fraction revealed the presence of saponins, tannins and phenolic compounds.
Conclusion: The study presents the first report of antifungal potential of Combretum latifolium and Humboldtia brunonis. High polar tannin is identified as the antifungal active principle in the aqueous extract of C. latifolium.
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References
Winslow LC, Kroll DJ. Herbs as medicine. Arch Intern Med 1998;158:2192-9.
Mudur G. Mandatory rural practice proposed in India. Br Med J 1995;311:1186.
Mudur G. Panel defends India’s traditional doctors. Br Med J 1997;314:1573.
Mehta DK, Martin J, Jordan B, Macfarlane CR, Hashimi FT, Kouimtzi M, et al. (Eds). British National Formulary. Pharmaceutical Press, London; 2008. p. 294-8.
Udayan PS. Floristic inventory in the seven medicinal plants conservation areas (MPCAs) of Karnataka, India. Ph. D Thesis, Bharathiar University, Coimbatore, Tamil Nadu; 2003.
Bhandary MJ, Chandrashekhar KR, Kaveriappa KM. Medical ethnobotany of Siddis of Uttara Kannada District, Karnataka, India. J Ethnopharm 1995;47:149-56.
Bhandary MJ, Chandrashekhar KR, Kaveriappa KM. Ethnobotany of gawlis of uttara kannada district, Karnataka. J Econ Taxon Bot 1996;12:244-9.
Bhat P, Hegde G, Hegde GR. Ethnomedicinal practices in different communities of Uttara Kannda district of Karnataka for treatment of wounds. J Ethnopharm 2012;143:501-14.
Martin GJ. Ethnobotany: a ‘people and plants’ conservation manual. Chapman and Hall, London; 1995.
Ganeshaiah KN, Ganesan R, Vasudeva R, Kushalappa CG, Menon ARR, Ankur Patwardhan, et al. Plants of Western Ghats Vol I-II. School of Ecology and Conservation, University of Agricultural Sciences, Bangalore; 2012.
Jain SK. Dictionary of Indian folk medicine and ethnobotany. Deep Publications, New Delhi; 1991.
Kirtikar KR, Basu BD. Indian medicinal plants vol I-II. Oriental Enterprises, Uttaranchal; 2001.
Yoganarasimhan SN. Medicinal Plants of India. vol I. Interline Publishing Private Limited, Bangalore; 1996.
Gupta VP, Govindaiah, Datta RK. Plant extracts: a non-chemical approach to control Fusarium diseases of mulberry. Curr Sci 1996;71:406-9.
Schwalbe R, Moore LS, Goodwin AC. Antimicrobial susceptibility testing protocols. CRC Press, Taylor and Francis Group, Boca Raton, London, New York; 2007.
Pavia DL, Lampman GM, Kriz GS, Engel RG. Introduction to organic laboratory techniques: a microscale approach. saunders college Publishing, a division of Holt, Rinehart and Wiston, New York; 1990.
Lee DG, Kim PI, Park Y, Woo E, Choi JS, Choi CH, et al. Design of novel peptide analogs with potent fungicidal activity, based on PMAP-23 antimicrobial peptide isolated from porcine myeloid. Biochem Biophys Res Commun 2002;293:31-8.
Jahn B, Martin E, Stueben A, Bhakdi S. Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide assay. J Clin Microbiol 1995;33:661-7.
Mosmann T. Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assays. J Immunol Meth 1983;65:55-63.
Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall, London; 1978.
Masoko P, Mdee LK, Mampuru LJ, Eloff JN. Biological activity of two related triterpenes isolated from Combretum nelsonii (Combretaceae) leaves. Nat Prod Res 2008;22 Suppl 12:1074-84.
Kurian A, Sankar MA. Medicinal plants. Horticulture Science Series-2. Edr Peter KV. New India Publishing Agency, New Delhi, India; 2007.
Rahalison L, Hamburger M, Hostettmann K, Monod M, Frenk E. A bioautographic agar overlay method for the detection of antifungal compounds from higher plants. Phytochem Anal 1991;2 Suppl 5:199-203.
Shrisha DL, Raveesha KA, Nagabhushan. Bioprospecting of selected medicinal plants for antibacterial activity against some pathogenic bacteria. J Med Plants Res 2011;5 Suppl 17:4087-93.
Harsha VH, Hebbar SS, Shripathi V, Hegde GR. Ethnomedicobotany of Uttara Kannada district in Karnataka, India-plants in treatment of skin diseases. J Ethnopharm 2003;84:37-40.
Eloff JN, Famakin JO, Katerene DRP. Combretum woodii (Combretaceae) leaf extracts have high activity against Gram-negative and Gram-positive bacteria. Afr J Biotechnol 2005;4 Suppl 10:1161-6.
Bombardelli E, Martinelli EM, Mustich G. Plants of Mosambique. VII triterpenoids of Terminalia sericea. Phytochem 1974;13:2559-62.
Carr JD, Roger CB. Chemosystematic studies of the genus Combretum (Combretaceae), A convenient method of identifying species of this genus by a comparison of the polar constituents extracted from leaf material. S Afr J Bot 1987;53:173-6.
Jossang A, Pousset JL, Bodo B. Combreglutinin, a hydrolysable tannin from Combretum glutinosum. J Nat Prod 1994;57 Suppl 6:732-7.
Malan E, Swinny E. Substituted bibenzyls, phenanthrenes and 9, 10-dihydrophenanthrenes from the heart wood of Combretum apiculatum. Phytochem 1993;34:1139-42.
Udem SC, Madubunyi I, Azuzu IU, Anika SM. The trypanocidal action of the root extract of Combretum dolichopetalum. Fitoterapia 1996;67:31-7.
Kolodziej H, Kayser O, Latte KP, Ferreira D. Evaluation of the antimicrobial potency of tannins and related compounds using the microdilution broth method. Planta Med 1999;65:444-6.