EVALUATION OF THE ANTIBACTERIAL ACTIVITY AND DETERMINATION OF POLYPHENOLS AND FLAVONOIDS CONTENTS OF MOROCCAN CORIARIA MYRTIFOLIA EXTRACTS
Keywords:
Antibacterial activity, Coriaria myrtifolia, Mycobacterium, Total polyphenols, FlavonoidsAbstract
Objective: This study aimed to evaluate the antibacterial activity of Coriaria myrtifolia's extracts from two Moroccan regions and to determine their total polyphenol's and flavonoid's contents.
Methods: The disk diffusion method was used to evaluate antimicrobial activity. Folin Ciocalteu and aluminum trichloride methods were used to determine the Total polyphenol's and flavonoid's contents respectively.
Results: The ethyl acetate extracts induced inhibition zone diameters of 20.3 mm and 22.6 mm for samples collected from Bab Berred (BB) and of 20 and 19 mm for those from Oued Al Koub (OK) respectively in Mycobacterium aurum and Mycobacterium smegmatis. Inhibition zone diameters of Pseudomonas aeruginosa were only 12.3 and 10 mm respectively for BB and OK ethyl acetate extracts. The polyphenols and flavonoids contents were respectively 609µg/ml and 105µg/ml in the OK ethyl acetate extract and only 475 and 6 µg/ml in the dichloromethan extract.
Conclusion: The antibacterial activity of tested extracts depends on the extract's nature, the bacterial strain and the plant's geographical provenance. The ethyl acetate's extract of C. myrtifolia from BB was the most active. Mycobacterium aurum and M. smegmatis were the most sensitive to Coriaria myrtifolia's extracts and P. aeruginosa was the most resistant. The polyphenol's and flavonoid's contents were different depending on the extract's nature and the plant's provenance. Valorization of Coriaria myrtifolia and evaluation of its biological and phytochemical activities will enable us to screen and test new natural antibacterial molecules in general and antimycobacterial agents in particular.
Â
Downloads
References
Djeridane A, Yousfi M, Nademi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem 2006;97:654-60.
Janovska D, Kubikova K, Kokosba L. Screening for antimicrobial activity of some medicinal plant species of traditional Chinese medicine. Czech J Food Sci 2003;21:107-10.
Thuille N, Fille M, Nagl M. Bactericidal activity of herbal extracts. Int J Hyg Environ Health 2003;206:217-21.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;10:564-82.
Vuotto LM, Basile A, Moscatiello V, Sole DP, Castaldo-Cobianchi R, Laghi E, et al. Antimicrobial and antioxidant activities of Feijoa sellowiana fruit. Int J Antimicrob Agents 2000;13:1997-201.
Farrapo MN, Gleidy Silva AA, Karine N, Costa, Magali Silva G, José Cogo C, et al. Inhibition of Bothropsjararacussu venom activities by Plathymenia reticulate Benth extracts. J Venom Res 2011;2:52-8.
Sqalli H, El Ouarti A, Ennabili A, Ibnsouda S, Farah A, Haggoud A, et al. Evaluation de l’effet antimycobactérien de plantes du centre-nord du Maroc. Bull Soc Pharm Bordeaux 2007;146:271-88.
NCCLS: National committee for clinical laboratory standards. Performance standards for antimicrobial disk susceptibility tests: Approved standard M02-A11. 11th ed. Wayne: Clinical and Laboratory Standards Institute; 2012. p. 67.
Singleton LV, Orthofer R, Lamuela-Raventos MR. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods Enzymol 1999;299:152-78.
Warburton E, Norris LP, Goenaga-Infante H. Comparison of the capabilities of accelerated solvent extraction and sonication as extraction techniques for the quantification of kavalactones in Piper methysticum (Kava) roots by high performance liquid chromatography with ultra violet detection. Phytochem Anal 2007;18:98-102.
Hezbri K, Hafsé M, Farah A, Sbissi I, Gtari M, Fikri Benbrahim K. Antibacterial activity of lyophilized aqueous extract of Coriaria myrtifolia from Northern Morocco. J Adv Biotechnol 2014;3(3):280-5.
Boudkhili M, Greche H, Bouhdid S, Zerargui F, Aarab L. In vitro antioxidant and antimicrobial properties of some Moroccan’s Medicinal plants. Int J Pharm Tech Res 2012;4:637-42.
Iauk L, Caccamo F, Speciale AM, Tempera G, Ragusa S, Pante G. Antimicrobial Activity of Rhus coriaria L. Leaf Extract. Phytother Res 1998;12:152-3.
Bolou EGK, Attiqua B, N’Guessan AC, Coulibaly A, N’Guessan JD, Djaman AJ. Évaluation in vitro de l’activité antibactérienne des extraits de Terminalia glaucescens planch. sur Salmonella typhi et Salmonella typhimurium. Bull Soc R Sci Liege 2011;80:772-90.
Aganga AA, Mosase KW. Tannins content nutritive value and dry matter digestibility of Lonchocarous capussa, Ziziphus mucropata, Sclerocarya birrea, Kirkia acuminata and Rhus lancea seeds. Anim Feed Sci Technol 2001:691;107-13.
Turkmen N, Sari F, Velioglu SY. Effects of extraction solvents on concentration antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem 2006;99:835-41.
Bazinet L, Labbe D, Tremblay A. Production of green tea EGC-and EGCG-enriched fractions by a two-step extraction procedure. Sep Purif Technol 2007;56:53-6.
Cetkovic GS, Canadovic-Brunet JM, Djilas MS, Tumbas TV, Markov LS, Cvetkovic DD. Antioxidant potential, lipid peroxidation inhibition and antimicrobial activities of Satureja montana L. subsp. Kitaibelii extracts. Int J Mol Sci 2007;8:1013-27.
Stefanakis MK, Touloupakis E, Anastasopoulos E, Ghanotakis D, Katerinopoulos EH, Makridis P. Antibacterial activity of essential oils from plants of the genus Origanum. Food Control 2013;34:539-46.