DEVELOPMENT, CHARACTERIZATION, AND EVALUATION OF THE ANTIMICROBIAL PROPERTIES OF BIODEGRADABLE POROUS SCAFFOLDS LOADED WITH NATURAL VANILLIN

R. M. AKILA; M. JANANI

doi:10.22159/ijpps.2023v15i11.48987

Authors

R. M. AKILA Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical and Sciences, Coimbatore
M. JANANI Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical and Sciences, Coimbatore

DOI:

https://doi.org/10.22159/ijpps.2023v15i11.48987

Keywords:

Biodegradable scaffolds, Porous scaffolds, Franz diffusion cell, Chitosan-Gelatin, Natural vanillin

Abstract

Objective: The study's objective is to create biodegradable porous scaffolds that are filled with natural vanillin and assess their in vitro antibacterial activity.

Methods: Scaffolds were fabricated by blending different ratios of chitosan and gelatin along with vanillin using the freeze-drying method. Then the following characterization and evaluation of scaffolds, such as FTIR, SEM, porosity, swelling behaviour, degradation studies, in vitro drug release, and antibacterial studies, were carried out.

Results: All of the scaffolds that were created had heterogeneous, well-connected pores and were pale yellow in color. This was validated by SEM, where the porosity is greater than 80% and the mean pore size ranges from 105.25±6.35 µm to 188.58±7.51 µm. With an increase in gelatin concentration, all of the scaffolds showed the maximum water absorption and retention capabilities of 760.15%±4.38% and 664.73%±5.82%. In the 7-day degradation investigation, all samples lost close to 60% of their mass. In the formulation CG11, the vanillin was released gradually over about 96 h. According to the present study, the developed scaffolds CG13-A and CG13-B, as well as CG11-A and CG11-B, displayed a higher zone of inhibition.

Conclusion: Due to its potent antibacterial capabilities, it may be inferred from the current research that vanillin clothed in chitosan-gelatin scaffolds would be a superior option for treating various wound infections, including diabetic wounds.

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