VARIATION IN ANTIOXIDANT POTENTIAL OF CURCUMA LONGA L. COLLECTED FROM DIFFERENT ECOLOGICAL NICHES OF WESTERN HIMALAYAN REGION
Keywords:
Free radical scavenging, Total antioxidant capacity, Total phenolic, Total flavonoid, Total ascorbic acidAbstract
Objective: The present study aims to evaluate the nutritional content and antioxidant activities of twenty accessions of turmeric rhizome from various locations of Uttarakhand.
Methods: The antioxidant activity was evaluated by different method viz; DPPH radical scavenging activity, ferric reducing antioxidant power assay (FRAP), Hydroxyl radical scavenging activity, Nitric Oxide (NO) scavenging activity, Superoxide anion radical scavenging activity and nutritional value viz; total phenolic content, total flavonoid content, total ortho-dihydroxy phenols, total ascorbic acid content and total antioxidant capacity.
Results: Alcoholic extracts of different turmeric rhizomes showed wide variation in abilities to scavenge nitric oxide, hydroxyl, superoxide free radical, DPPH with IC50 values in the range of 39.03-76.14, 39.77-72.85, 43.77-77.13, 64.38-271.95 µg/ml and FRAP value 19.54-54.84 µmole. The total phenolic content, total flavonoid content, total ortho-dihydroxy phenols, total ascorbic acid content and total antioxidant capacity of different alcoholic extracts of rhizomes were found in the range of 11.19-51.49, 6.35-30.95, 0.93-8.12, 0.14-1.29 and 27.44-94.78 mg/g respectively.
Conclusion: The alcoholic extract of different accessions of Curcuma longa L. has shown excellent biochemical and antioxidant potential and therefore refers as an important natural source of antioxidant.
Â
Downloads
References
Cai YZ, Sun M, Corke H. Antioxidant activity of betaline from plants of the Amaranthacea. J Agric Food Chem 2003;51:2288-94.
Lim YY, Murtijaya J. Antioxidant properties of Phyllanthus amarus as affected by different drying methods. lebensmittle wissenschaft. Technol 2007;40:1664-9.
Keawpradub N, Zaeoung S. Cytotoxic and free radical scavenging activities of Zingiberaceous rhizomes. Songklanakarin J Sci Technol 2005;27:799-812.
Stoilova I, Krastanov A, Stoyanova A, Denev P, Gargova S. Antioxidant activity of ginger extract. Food Chem 2007;102:764-70.
Ravindran PN. Turmeric the golden spice of life. Turmeric the genus Curcuma. Taylor Francies 2006;14(1):1-14.
Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin; the Indian solid golid. Adv Exp Med BIol 2007;595:1-75.
Tang W, Eisenbrand G. Chinese drugs of plant origin. Springer Verlag, Berlin, Heidelberg. Germany 1992;613-9.
Lee H-S, Shin WK, Song C, Cho KY, Ahn YJ. Insecticidal activities of ar-turmerone identified Curcuma longa L. rhizome against Nilaparvata lugens (Homoptera: Delphacidae) and Plutella xyostella (Lepiodoptera: Yponomeutidae). J Asia-Pac Entomol 2001;4(2):181-5.
Wang Y, Lu Z, Wu H, Lv F. Study on the antibiotic activity of microcapsule curcumin aganist foodborn pathogens. Int J Food Microbiol 2009;136:71-4.
Kuttan R, Bhanumathly P, Nirmala K, George MC. Potential anticancer activity of turmeric (Curcuma longa). Cancer Lett 1985;29(2):197-202.
Wang X, Jiang Y, Wang YW, Huang MT, Ho CT, Hauang Q. Enhancing anti-inflammation activity of curcumin through O/W nanoemulsions. Food Chem 2008;108:419-24.
Ammon HPI, Wahl MA. Pharmacology of Curcuma longa L. Plant Med 1991;57:1-7.
Kiso Y, Suzuki H, Watanabe N, Oshima Y, Hikino O. Antihepatotoxic principles of Curcuma longa rhizomes. Plant Med 1983;49:185-7.
Devasagayam TPA, Sainis KB. Immune system and antioxidants especially those derive from Indian medicinal plants. Indian J Exp Biol 2002;40:639-55.
Jayaprakasha GK, Rao LJ, Sakariah KK. Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Food Chem 2006;98:720-4.
Maheshwari RK, Shing AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin; A short review. Life Sci 2006;78:2081-7.
Singlenton UL, Rossi JA. Colorimetry of total phenolics with phosphomolibdic phosphotungstic acid reagent. Am J Enol Vitic 1965;16:144-58.
Choi Y, Jeong H, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 2007;130:130-8.
Arnow LE. Colorimetric determination of the components of 3,4-dihydroxyphenylalanin-tyrosine mixtures. J Biochem 1937;118:531-7.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.
Reo JH, Kuether CA. Determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivate of ascorbic acid. J Biochem 1943;143:399-406.
Benzie IFF, Strain JJ. The ferric reducing ability of plasma as a measure of antioxidant power; the FRAP assay. Anal Biochem 1996;239:70-6.
Braca A, Sortino C, Politi M. Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol 2002;79:379-81.
Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method; a simple test-tube†assay for determination of rate constants for reaction of hydroxyl radicals. Anal Biochem 1987;165:216-9.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JK, Tannenbaum SR. Analysis of nitrate, nitrite and 15N in biological fluids. Anal Biochem 1982;126:131–6.
Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide scavenging property of Ginkgo biloba extract Egb 761. Biochem Biophys Res Commun 1994;201:748-55.
Nishimiki M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 1972;46:849-53.
Bryant JP, Chapin FSI, Klein DR. Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. Oikos 1983;40:357-68.
Dixon RA, Paiva NL. Stress-induced phenyepropanoid metabolism. Plant Cell 1995;7(7):1085-97.
Zhang W, Seki M, Furusaki S. Effect of temperature and its shift on growth and anthocyanin production in suspension cultures of strawberry cells. Plant Sci 1997;127:207-14.
Chalker-Scott L, Fnchigami LH. The role of phenolic compounds in plant stress responses; low temperature stress physiology in crops (paul HL. Ed. CRC press: Inc, Boca Raton, Florida Entomologist; 1989. p. 40.
Hotelling H. Analysis of a complex of statistical variables into principal components. J Educational Psychology 1933;24:417-41.
Lohani M, Singh D, Singh JP. Genetic diversity assessment through principal component analysis in potato. Veg Sci 2012;39(2):207-9.