BIOCHEMICAL AND HISTOLOGICAL STUDY OF THREE DIFFERENT MULTIHERBAL COMPOSITIONS ON HEPATOXICITY INDUCED BY CARBON TETRA CHLORIDE (CCL4) IN ALBINO RATS

SHEKHAR KUMAR SINORIYA; KUSUM SINGH

doi:10.22159/ijpps.2024v16i10.51203

Authors

SHEKHAR KUMAR SINORIYA Department of Zoology, Institute of Basic Science, Bundelkhand University, Jhansi (U.P.), 284128, India https://orcid.org/0009-0005-6761-3009
KUSUM SINGH Department of Zoology, Institute of Basic Science, Bundelkhand University, Jhansi (U.P.)-284128, India

DOI:

https://doi.org/10.22159/ijpps.2024v16i10.51203

Keywords:

Hepatoxicity, CCl4, Multiherbal formulations, Serum glutamate pyruvate transaminase (SGPT), Alanine transaminase (ALT), Serum glutamic oxaloacetic transaminase (SGOT), Aspartate transaminase (AST), Alkaline Phosphatase (ALP), Central Vein (CV), Sinusoids (S), and Hepatocytes (H)

Abstract

Objective: The present study aimed to evaluate the hepatoprotective activity of three different types of multiherbal formulations in comparison to standard drug (Silymarin).

Methods: Five hepatoprotective plants were chosen to make three different kinds of formulations, as mentioned in the materials and methods. These five selected herbs were chosen based on previous researches or reviews on hepatoprotective herbs. For the present study, two different groups of rats were made: the control group and the experimental group. The experimental group was further divided into five subgroups. Hepatotoxicity was induced by a single oral dose (1.5 ml/kg) of CCl₄. After this, all rats were treated with different formulations and standard drug (silymarin) according to their groups. Samples for biochemical and histopathological (liver sample) examinations were collected on the fixed schedules (07^th, 14th, and 21st days).

Results: Biochemical parameters like SGPT, SGOT, and ALP were elevated due to CCl₄-induced hepatotoxicity, and after treatment, they were recouped to normal significantly (P<0.001) by the treatment of formulation-I. On the other hand, decreased serum proteins like total, albumin, and globulin were increased to be normal (P<0.01) with the treatment of formulation-I. The histopathological study also supported the final results of the biochemical analysis. The damaged hepatocellular structures were repaired by the F-I formulation better than other formulations.

Conclusion: The final results suggested that Formulation-I is a better healer than other formulations (F-II and F-III). It was thus concluded in the present study, that formulation-I has better hepatoprotective activity than formulation-II and formulation-III.

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