InstaPRESERVETM: AN ALTERNATIVE FIXATIVE TO FORMALIN AND SUITABLE FOR HISTOPATHOLOGY

RAJANI KANTH VANGALA; ANAND BABU VANGALA; SARANYA KANUKOLLU; PRATIBHA M. S.; NITHUN REDDY C.; AVINASH S.; JEEVAPRIYA P.; JINY NAIR; PRAMOD N. NAIR; ELANGO E. MURUGAIAN

doi:10.22159/ijpps.2024v16i8.51552

Authors

RAJANI KANTH VANGALA Neuome Technologies Private Limited, Electronic City, Bangalore, India
ANAND BABU VANGALA Neuome Technologies Private Limited, Electronic City, Bangalore, India
SARANYA KANUKOLLU Neuome Technologies Private Limited, Electronic City, Bangalore, India https://orcid.org/0000-0003-0614-3566
PRATIBHA M. S. Neuome Technologies Private Limited, Electronic City, Bangalore, India https://orcid.org/0009-0006-1444-2610
NITHUN REDDY C. Spectrum Diagnostics, Bangalore, India
AVINASH S. Neuome Technologies Private Limited, Electronic City, Bangalore, India
JEEVAPRIYA P. Neuome Technologies Private Limited, Electronic City, Bangalore, India
JINY NAIR Neuome Technologies Private Limited, Electronic City, Bangalore, India https://orcid.org/0000-0001-6745-5673
PRAMOD N. NAIR Neuome Technologies Private Limited, Electronic City, Bangalore, India https://orcid.org/0000-0002-2918-4929
ELANGO E. MURUGAIAN Neuome Technologies Private Limited, Electronic City, Bangalore, India https://orcid.org/0000-0001-6902-1983

DOI:

https://doi.org/10.22159/ijpps.2024v16i8.51552

Keywords:

Histopathology, Formalin, Fixative, Formalin alternative

Abstract

Objective: To evaluate the efficacy of InstaPRESERVE^TM for replacing formalin in histopathology.

Methods: Tissue from seven organs from a pig was fixed with InstaPRESERVE^TM and formalin for 6 hrs, 12 hrs, and 24 hrs. Microtome sections at three time points were stained with hematoxylin & eosin and evaluated for intactness, and nuclear and cytoplasmic staining. Scoring was done based on their quality.

Results: Gross findings were well appreciated for the tissue specimens fixed with InstaPRESERVE^TM compared to formalin. Unlike formalin, discoloration was lesser and the rigidity was mild to firm with InstaPRESERVE^TM fixation. The penetration rate of InstaPRESERVE^TM was equivalent to formalin and the morphology and intactness were well preserved. The nuclear and cytoplasmic H&E staining intensity of InstaPRESERVE^TM fixed tissues was comparable to formalin fixation.

Conclusion: The promising results from InstaPRESERVE^TM fixed tissues of pig organs are encouraging. This preliminary study is positive on replacing formalin in routine histopathology thus preventing the pathology laboratory personnel from occupational hazards. Further studies with human specimens are required to escalate InstaPRESERVE^TM to medical histopathology.

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