BIOREMEDIATION OF SANITARY NAPKIN BY CELLULOSE DEGRADING BACTERIA
DOI:
https://doi.org/10.22159/ijpps.2024v16i8.51669Keywords:
Carboxy methyl cellulose (CMC), Cellulase-producing bacteria, Consortium, Bioremediation, Sanitary napkinAbstract
Objective: This research aims to isolate cellulase-producing microbes from ruminants and investigate their potential for bioremediation of organic wastes, like sanitary napkins. Organic waste management is a critical environmental challenge, and bioremediation offers a sustainable approach for waste treatment. Ruminant animals possess a unique microbial population in their digestive systems that can efficiently degrade cellulose, a major component of sanitary napkins.
Methods: In this study, samples of garden soil, cow dung, buffalo dung, and dumping yard soil were collected and screened for cellulase-producing microbes using Carboxy Methyl Cellulose (CMC) agar medium. Subsequently, the cellulase-producing microbes were employed in the Whattman filter paper degradation and their capacity to degrade the cellulose in it by performing a DNSA assay. Furthermore, these isolates were employed in the bioremediation process to degrade sanitary napkins. Thereafter, we prepared various consortia of the isolates to check if it led to better degradation of sanitary napkins.
Results: The results demonstrated the successful isolation of cellulase-producing microbes from all the samples using CMC agar medium and were labeled as Isolates 1, 2, 3, 4, G, and D. In the filter paper degradation assay, isolate 3 produced the highest amount of reducing sugar from 0.1 g of cellulose, followed by isolate G, indicating the highest cellulase or FPase activity among all isolates. Additionally, these isolates exhibited promising potential for the degradation of sanitary napkins. Tube with isolate 3 had the highest concentration of reducing sugar and the lowest dry weight of sanitary napkin, followed by isolate G. Isolates 3 and G showed promising results as compared to the other isolates, but isolated 3 had an antagonistic effect when it was used with other isolates in the consortium. In contrast, isolate G showed synergistic effects in the consortium, and G+D showed the highest degradation of sanitary napkins.
Conclusion: This research contributes a microbial-based bioremediation approach to the development of sustainable and environmentally friendly strategies for waste management.
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References
Mango P. The future of global nonwoven wipes market forecasts to 2023. In: 57th Dornbirn Global Fiber Congress: Dornbirn, Austria; 2018.
Reyes NJDG, Geronimo FKF, Yano KAV, Guerra HB, Kim LH. Pharmaceutical and Personal Care Products in Different Matrices: Occurrence, Pathways, and Treatment Processes. Water. 2021;13(9):1159.
Kjellen M, Pensulo C, Nordquist P, Fogde M. Global Review of Sanitation System Trends and Interactions with Menstrual Management Practices. SEI Project Report: Stockholm, Sweden; 2012.
Siddiqua A, Hahladakis JN, Al-Attiya WAKA. An overview of the environmental pollution and health effects associated with waste landfilling and open dumping. Environ Sci Pollut Res Int. 2022;29:58514-58536.
Malladi R, Malladi N, Mathieu R, Saïd E. Importance of Agricultural and Industrial Waste in the Field of Nanocellulose and Recent Industrial Developments of Wood Based Nanocellulose: A Review. ACS Sustainable Chem Eng. 2018;6(3):2807-2828.
Neelamegam A, Rajeswari M, Thangavel B. Endo-1,4-β-glucanases: Role, Applications and Recent Developments. In: Gupta VK, editor. Microbial Enzymes in Bioconversions of Biomass. Springer International Publishing: Switzerland; 2016. p. 37-45.
Dienes D, Egyhazi A, Reczey K. Treatment of recycled fiber with Trichoderma cellulases. Ind Crop Prod. 2004;20(1):11-21.
Narendrakumar G, Saikrishna NMD, Prakash P, Preethi TV. Analysis of gut flora from damp wood termites (trinervitermes spp.) and extraction, characterization of cellulase from the isolate. Asian J Pharm Clin Res. 2017;10(6):233-236.
Das KC, Qin W. Isolation and characterization of superior rumen bacteria of cattle (Bos taurus) and potential application in animal feedstuff. Open J Anim Sci. 2012;2(4):224-228.
Jindou S, Borovok I, Rincon MT, Flint HJ, Antonopoulos DA, Berg ME, White BA, Bayer EA, Lamed R. Conservation and divergence in cellulosome architecture between two strains of Ruminococcus flavefaciens. J Bacteriol. 2006;188:7971–7976.
Nyonyo T, Shinkai T, Mitsumori M. Improved culturability of cellulolytic rumen bacteria and phylogenetic diversity of culturable cellulolytic and xylanolytic bacteria newly isolated from the bovine rumen. FEMS Microbiol Ecol. 2014;88(3):528–537.
Arshad K, Skrifvars M, Vivod V, Valh JV, Vonˇcina B. Biodegradation of natural textile materials in soil. Tekstilec. 2014;57:118–132.
Hema JN, Shobha, Shruthi SD. Isolation and characterization of cellulose-degrading bacteria from decomposing plant matter. Int J Pharm Pharm Sci. 2023;15(4):22-27
Gupta P, Samant K, Sahu A. Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. Int J Microbiol. 2012;2012:578925,1-5.
Gayathri B, Gayathri V. Formulation of Bacterial and Fungal Isolates for the Degradation of Sanitary Napkins. Asian J Microbiol Biotech Env Sci. 2018;20(2): S230-S236.
Patel AK, Singhania RR, Sim SJ, Pandey A. Thermostable cellulases: current status and perspectives. Bioresour Technol. 2019;279:385-392.
Ajeje SB, Hu Y, Song G, Peter SB, Afful RG, Sun F, Asadollahi MA, Amiri H, Abdulkhani A, Sun H. Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective. Front Bioeng Biotechnol. 2021;9:794304.
Ekperigin M.M. Preliminary studies of cellulase production by Acinetobacter anitratus and Branhamella sp. Af J Biotechnol. 2007;6(1):28–33.
Fujimoto N, Kosaka T, Nakao T, Yamada M. Bacillus licheniformis bearing a high Cellulose-Degrading Activity, which was isolated as a Heat-Resistant and Micro-Aerophilic Microorganism from Bovine. The Open Biotechnol J. 2011;5:7-13.
Lu W. Wang HT, Nie Y, Wang ZC, Huang De-Y, Qiu XY, Chen JC. Effect of inoculating flower stalks and vegetable waste with ligno-cellulolytic microorganisms on the composting process. J Environ Sci Health B. 2004;39:871– 887.
Bichet-Hebe I, Pourcher AM, Sutra L, Comel C, Moguedet G. Detection of a whitening fluorescent agent as an indicator of white paper biodegradation: a new approach to study the kinetics of cellulose hydrolysis by mixed cultures. J Microbiol Methods. 1999;37(2):101–109.
Nanda S, Sahu S, Abraham J. Studies on the biodegradation of natural and synthetic polyethylene by Pseudomonas sp. J Appl Sci Environ Manage. 2010;14(2):57-60.
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