ACUTE TOXICITY STUDY OF ARSENICUM ALBUM IN WISTAR ALBINO RATS
DOI:
https://doi.org/10.22159/ijpps.2024v16i12.52513Keywords:
Arsenicum album, Acute toxicity, Covid, Herbal medicineAbstract
Objective: In India, homeopathic medicines derived from arsenic trioxide, such as Arsenicum album (A. album), are used to treat COVID-19. Many of the Arsenicum album's adverse events during the COVID treatment led to drug discontinuation. Nonetheless, Ayurvedic, Yoga and Naturopathy, Unani, Siddha, and Homoeopathic (AYUSH) medicine prescribes it. Researching Arsenicum album's toxicity is now essential since it will help decide whether or not the drug can be utilized during treatment.
Methods: Acute oral toxicity was performed in Wistar Albino Rats to find out the effects of Arsenicum album in various organs. Rats were divided into three groups: Group A (control), Group B (Arsenicum album 1000μl/100g) and Group C (2000μl/100g). A Single bolus dose of Arsenicum album was given orally and the study period was conducted for 14 d. The rats were subsequently sacrificed on the 15th d and biochemical and histopathological studies were done.
Results: The acute oral toxicity study showed median Lethal dose (LD50) was greater than 2000μl/100g for Arsenicum album. Biochemical analysis showed a significant increase in HDL level in Group C (2000μl/100g) compared to Group A (control) and Group B (1000μl/100g). [P<0.05]. Blood glucose, when compared to Group A (control) is increased in both groups B and C but statistically not significant (p>0.05). When compared to Group A (control) there is no significant change in blood urea level in Groups B and C (p>0.05). Serum creatinine is increased in Groups B and C compared to Group A (control) but statistically not significant (p>0.05). LDL cholesterol is increased in groups B and C compared to Group A (control) but statistically not significant (p>0.05). Macroscopic examination of the organs of Group B and C revealed no abnormalities when compared with the organs of Group A (control). Histopathological analysis showed mild hepatocellular and renal toxicity in Groups B and C with extensive hemorrhages and periportal lymphocytic infiltrates.
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References
Firenzuoli F, Gori L, Crupi A, Neri D. Flavonoids: risks or therapeutic opportunities? Recenti Prog Med. 2004 Jul 1;95(7-8):345-51. PMID 15303543.
Swayn international dictionary of homeopathy. Edinburgh: Churchill Livingstone; 2008.
Ernst E. Homoeopathy: past present and future. Br J Clin Pharmacol. 1997;44(5):435-7. doi: 10.1046/j.1365-2125.1997.t01-1-00611.x, PMID 9384459.
Linde K, Clausius N, Ramirez G, Melchart D, Eitel F, Hedges LV. Are the clinical effects of homeopathy placebo effects? A meta-analysis of placebo-controlled trials. Lancet. 1997 Sep 20;350(9081):834-43. doi: 10.1016/s0140-6736(97)02293-9, PMID 9310601.
Tillu G, Chaturvedi S, Chopra A, Patwardhan B. Public health approach of ayurveda and yoga for COVID-19 prophylaxis. J Altern Complement Med. 2020 May 1;26(5):360-4. doi: 10.1089/acm.2020.0129, PMID 32310670.
Liu SY, Song JC, Mao HD, Zhao JB, Song Q, Expert Group of Heat Stroke Prevention and Treatment of the People’s Liberation Army, and People’s Liberation Army Professional Committee of Critical Care Medicine. Expert consensus on the diagnosis and treatment of heat stroke in China. Mil Med Res. 2020;7(1):1. doi: 10.1186/s40779-019-0229-2, PMID 31928528.
Guan WJ, Ni ZY, HU Y, Liang WH, OU CQ, HE JX. Clinical characteristics of coronavirus disease 2019 in China. N Engl J Med. 2020;382(18):1708-20. doi: 10.1056/NEJMoa2002032, PMID 32109013.
Jethani B, Gupta M, Wadhwani P, Thomas R, Balakrishnan T, Mathew G. Clinical characteristics and remedy profiles of patients with COVID-19: a retrospective cohort study. Homeopathy. 2021;110(2):86-93. doi: 10.1055/s-0040-1718584, PMID 33567460.
Velu V, Uthandakalaipandian R. Efficacy of microdoses potentized homeopathic drug arsenicum album induced by arsenic trioxide in zebrafish (Danio rerio) a FTIR study in gills. Int J Pharm Pharm Sci. 2016;9(1):48. doi: 10.22159/ijpps.2017v9i1.15314.
Samuel S, Kathirvel R, Jayavelu T, Chinnakkannu P. Protein oxidative damage in arsenic induced rat brain: influence of DL-α-lipoic acid. Toxicol Lett. 2005;155(1):27-34. doi: 10.1016/j.toxlet.2004.08.001, PMID 15585356.
Ive EC, Couchman IM, Reddy L. Therapeutic effect of arsenicum album on leukocytes. Int J Mol Sci. 2012;13(3):3979-87. doi: 10.3390/ijms13033979, PMID 22489193.
Variya BC, Bakrania AK, Madan P, Patel SS. Acute and 28 d repeated dose sub-acute toxicity study of gallic acid in albino mice. Regul Toxicol Pharmacol. 2019;101:71-8. doi: 10.1016/j.yrtph.2018.11.010.
Ekanayake CP, Thammitiyagodage MG, Padumadasa S, Seneviratne B, Padumadasa C, Abeysekera AM. Acute and subacute toxicity studies of the ethyl acetate soluble proanthocyanidins of the immature inflorescence of Cocos nucifera L. in female wistar rats. BioMed Res Int. 2019;2019:8428304. doi: 10.1155/2019/8428304, PMID 31886260.
Al Afifi NA, Alabsi AM, Bakri MM, Ramanathan A. Acute and sub-acute oral toxicity of dracaena cinnabari resin methanol extract in rats. BMC Complement Altern Med. 2018;18(1):50. doi: 10.1186/s12906-018-2110-3, PMID 29402248.
Bhushan B, Sardana S, Bansal G. Acute and sub-acute toxicity study of Clerodendrum inerme Jasminum mesnyi hance and callistemon citrinus. J Acute Dis. 2014;3(4):324-7. doi: 10.1016/S2221-6189(14)60069-X.
Ben Arye E, Samuels N, Goldstein LH, Mutafoglu K, Omran S, Schiff E. Potential risks associated with traditional herbal medicine use in cancer care: a study of Middle Eastern oncology health care professionals. Cancer. 2016;122(4):598-610. doi: 10.1002/cncr.29796, PMID 26599199.
Singh S, Kalra P, Karwasra R, Khurana A, Manchanda R, Gupta Y. Safety studies of homoeopathic drugs in acute sub-acute and chronic toxicity in rats. Indian J Res Homoeopathy. 2017;11(1):48-57. doi: 10.4103/ijrh.ijrh_68_16.
Panigrahy S, Dass D, Pandey SK, Sheena DM. Self medication for prevention and treatment of diseases during COVID-19 pandemic a cross-sectional survey in general population. Int J Pharm Pharm Sci. 2024;16(6):33-8. doi: 10.22159/ijpps.2024v16i6.50520.
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Copyright (c) 2024 MONISHA A., BHUVANESHWARI S., VELARUL S., SATHIYA VINOTHA A. T., UMAMAGESWARI M. S., VIJAYAMATHY A., KARTHIKEYAN T. M.
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