ISOLATION AND CHARACTERIZATION OF SHATAVARIN IV FROM ROOT OF ASPARAGUS RACEMOSUS WILLD
Keywords:
Asparagus racemosus Willd, Column Chromatography, Shatavarin IV, HPTLC, SpectroscopyAbstract
Objective: To develop a method for the isolation of the steroidal saponin Shatavarin IV from the root of Asparagus racemosus Willd.
Methods: Powder of Asparagus racemosus dried roots were defatted with hexane and extracted with methanol. The methanolic extract was used for isolation of pure compound by column chromatography. Isolated compound was identified by melting point analysis, IR and Mass spectroscopic analysis. Purity was checked by High-performance thin layer chromatography (HPTLC).
Results: The highest yield of Shatavarin IV was obtained 401.1 mg from 250 g crude powder and highest purity was achieved 66% of lower atmospheric temperature (17-22 °C).
Conclusion: Shatavarin IV isolation using column chromatography was affected with the change in atmospheric temperature. Maximum yield was obtained at the lower temperature i.e. 17-22 °C, whereas at higher temperature the Shatavarin IV showed degradation in purity and yield.
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References
Sharma PV, Dravyaguna Vijnana. Vol. II (in Hindi), reprint, Chaukambha Bharti Academy, Varanasi; 2005.
Anonymous. The Ayurvedic Formulary of India. Vol 1. 1st Edition. New Delhi, Min Health, Govt of India; 1976.
Velavan S, Nagulendran KR, Mahesh R, Hazeena Begum. The Chemistry, Pharmacological and therapeutic applications of Asparagus racemosus-A Review. Pharmacogn Rev 2007;1(2):350-60.
Ravikumar PR, Soman R, Chetty GL, Pandey RC, Sukhdev. Chemistry of Ayurvedic crude drugs: Part VIa-(Shatavari-1): structure of Shatavarin-IVb,c. Indian J Chem 1987;26(B):1012–7.
Sachan AK, Das DR, Dohare SL, Shuaib M. Asparagus racemosus (Shatavari): An Overview. Int J Pharm Chem Sci 2012;1(3):588-92.
Satti NK, Suri KA, Prabhu Dutt, Suri OP, Musarat Amina, Qazi GN. Evaluation of Asparagus racemosus on the basis of immunomodulating sarsasapogenin glycosides by HPTLC. J Liq Chromatogr Relat Technol 2006;29:219–27.
Sigma-Aldrich. Safety data sheet according to regulation (EC); 2006.
Penumajji S, Bobbarala V, Kandula M, Laxmanraju G. Comparison of evaporative light scattering detector with UV detection system in the molecular marker compounds analysis of Asparagus racemosus. J Global Pharm Tech 2010;2(1):89-93.
Feng J, Chen D, Quan-zhong sun, Nakamura N, Hattor M. Asparoside A and B, Two new steroidal saponins from Asparagus meioclados. J Asian Nat Products Res 2002;4(3):221–6.
Jadhav AN, Bhutani KK. Steroidal saponins from the Roots of Asparagus adscendens Roxb and Asparagus racemosus Willd. Indian J Chem 2006;45B:1515-24.