QUANTIFICATION OF PHYTOCHEMICAL CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF EXACUM BICOLOR (ROXB.), AN ENDEMIC MEDICINAL PLANT
Keywords:
Exacum bicolor, DPPH, Nil, NO scavenging assay, CorrelationAbstract
Objective: To investigate different phytochemical contents and screening of antioxidant activity of sequentially extracted Exacum bicolor leaf with different solvents.
Methods: Leaf samples of E. bicolor were dried and subjected to sequential (hexane>chloroform>ethyl acetate>methanol>water) extraction using soxhlet apparatus. Total phenol contents (TPC) were estimated using Folin-ciocalteau method, flavonoids by aluminium chloride method, tannins by Folin-Denis reagent and alkaloids using bromocresol blue. In vitro antioxidant activities were estimated by using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid ABTS·+and Nitric oxide (NO) scavenging assay.
Results: The total phenol, tannin and alkaloid contents in methanol extract were found to be 23.4±0.09 mg GAE/g, 23±0.57 mg TAE/g and 47.33±1.20 mg AE/g respectively. Hexane extract exhibited high flavonoid content (27.6±0.88 mg QE/g). Methanolic extracts exhibited dose dependent antioxidant activity against DPPH, ABTS·+and NO Scavenging methods; low IC50 value (14.84 µg/ml) was observed in DPPH scavenging activity. Findings showed positive and significant (P ≤ 0.05) correlation between TPC/DPPH (R2= 0.98).
Conclusions: The methanol extract showed higher antioxidative activity compared to other extracts. Positive correlation was obtained between TPC and DPPH activity signifying that the phenolic content may be responsible for the antioxidant activity of the plant. Further bioassay-guided purification of the bioactive compound is necessary. To the best of our knowledge, antioxidant activity and its correlation with TPC, is reported for the first time in E. bicolor.
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References
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