• Appaji M Ashwini Jain University
  • Mala Majumdar JAIN UNIVERSITY


Exacum bicolor, DPPH, Nil, NO scavenging assay, Correlation


Objective: To investigate different phytochemical contents and screening of antioxidant activity of sequentially extracted Exacum bicolor leaf with different solvents.

Methods: Leaf samples of E. bicolor were dried and subjected to sequential (hexane>chloroform>ethyl acetate>methanol>water) extraction using soxhlet apparatus. Total phenol contents (TPC) were estimated using Folin-ciocalteau method, flavonoids by aluminium chloride method, tannins by Folin-Denis reagent and alkaloids using bromocresol blue. In vitro antioxidant activities were estimated by using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid ABTS·+and Nitric oxide (NO) scavenging assay.

Results: The total phenol, tannin and alkaloid contents in methanol extract were found to be 23.4±0.09 mg GAE/g, 23±0.57 mg TAE/g and 47.33±1.20 mg AE/g respectively. Hexane extract exhibited high flavonoid content (27.6±0.88 mg QE/g). Methanolic extracts exhibited dose dependent antioxidant activity against DPPH, ABTS·+and NO Scavenging methods; low IC50 value (14.84 µg/ml) was observed in DPPH scavenging activity. Findings showed positive and significant (P ≤ 0.05) correlation between TPC/DPPH (R2= 0.98).

Conclusions: The methanol extract showed higher antioxidative activity compared to other extracts. Positive correlation was obtained between TPC and DPPH activity signifying that the phenolic content may be responsible for the antioxidant activity of the plant. Further bioassay-guided purification of the bioactive compound is necessary. To the best of our knowledge, antioxidant activity and its correlation with TPC, is reported for the first time in E. bicolor.



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Rangari VD. Pharmacognosy and phytochemistry. 1st ed. Nashik: Carrier Publication; 2002.

Kaminidevi S, Thangavelu T, Lakshmanan A, Gunashekar D, Chirayil, Jamuna HT, et al. Preliminary phytochemical screening and antimicrobial activity of fresh plant extract of Indian folk medicinal plant, Gnaphalium polycaulon. Int J Phytomed 2014;6(1):82-6.

Shanmugapriya K, Udayabhanu J, Thayumanavan T. Investigation of antioxidant activity in different solvents of Gnaphalium polycaulon. Int J Pharm Pharm Sci 2014;6(2):870-3.

Grice HC. Safety evaluation of butylated hydroxyl toluene (BHT) in liver, lung and gastrointestinal tract. Food Chem Toxicol 1988;24:1127-30.

Franco D, Sineiro J, Rubilar M, Sánchez M, Jerez M. Polyphenols from plant materials: extraction and antioxidant power. Electron. J Environ Agric Food Chem 2008;7(8):3210-6.

Naczk M, Shahidi F. Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. J Pharm Biomed Anal 2006;41(5):1523-42.

Brilliant R, Vincy MV, Joby P, Pradeepkumar AP. Vegetation analysis of Montane forest of Western Ghats with special emphasis on RET species. Int J Biodiversity and Conserv 2012;4(15):652-64.

Sreelatha U, Baburaj TS, Kutty NC, Nazeem P, Bhaskar J. Cultivation prospects of Exacum bicolor Royle: an endangered, ornamental & anti-diabetic herb. Nat Prod Rad 2007;6(5):402-4.

Marles RJ, Farnsworth NR. Antidiabetic plant and their active constituents. Phytomed 1995;2:137-9.

Khare CP. Indian medicinal plants-an illustrated dictionary. Berlin: Springer; 2007. p. 77.

Reddi STV, Naidu BVAR, Prasanthi S. In: Herbal remedies for diseases, Alikhan and A Khanum 1st ed. Hyderabad: Ukay Publications; 2005. p. 67-13.

Megoneitso, Rao RR. Ethnobotanical studies in Nagaland medicinal plants used by the angami nagas. J Economic Taxonomic Botany 1983;4:167-72.

Dey A, De JN. Traditional use of medicinal plants as febrifuge by the tribals of Purulia district, West Bengal, India. Asia Pac J Trop Dis 2012;2(2):800-3.

Siddamallayya N, Yasmeen A, Gopakumar K. Medico-botanical survey of Kumara parvatha Kukke Subramanya. Indian J Traditional Knowledge 2010;9(1):96-9.

Dey A, Gupta B, De JN. Traditional phytotherapy against skin diseases and in wound healing of the tribes of Purulia district, West Bengal, India. J Med Plant Res 2012;6(33):4825-31.

Ashwini AM, Majumdar M. Qualitative phytochemical screening and in vitro anthelmintic activity of Exacum bicolor Roxb., an endemic medicinal plant from Western Ghats in India. Acta Biol Indian 2014;3(1):510-4.

Das S, Barua RN, Sharma RP, Baruah JN, Kulanthaivela P, Herza W. Secoiridoids from Exacum tetragonum. Phytochem 1984;23:908-9.

Singleton VL, Rossi JA. Colorimetry of phenols with phosphomolybdic–phospotungstic and reagents. Am J Enol Vitic 1965;37:144-8.

Choi CW, Kim SC, Hwang SS, Choi BK, Hye J. Antioxidant activity and free radical scavenging capacity between Korean medicial plants and flavonoids by assay-guided comparison. Plant Sci 2002;163(6):1161-8.

Polshettiwar SA, Ganjiwale RO. Spectrophotometric estimation of total tannins in some ayurvedic eye drops. Indian J Pharm Sci 2007;69(4):574-6.

Shamsa F, Monsef H, Ghamooshi R, Verdian-rizi M. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci 2008;32:17-20.

Braca A, Politi M, Sanogo R, Sanou H, Morelli I, Pizza C, et al. Chemical composition and antioxidant activity of phenolic compounds from wild and cultivated Sclero caryabirrea (Anacardiaceae) leaves. J Agric Food Chem 2003;51:6689–95.

Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 2006;94(4):550–7.

Sreejayan N, Rao MNA. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997;49:105-7.

Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longevity 2009;2(5):270-8.

Misra TK, Saha A. Soil sampling in tea plantation for fertility evaluation: A guideline. Assam Rev Tea News 2008;97:12-5.

Ahmad N, Mukhtar H. Green tea polyphenols and cancer: biological mechanisms and practical implications. Nutr Rev 1999;57(3):78-83.

Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Tech Int 2002;8(3):121–37.

Chen YH, Chang FR, Lin YJ, Hsieh PW. Identification of antioxidants from rhizome of Davallia solida. Food Chem 2008;107:684-91.

Sharma N, Varshney VK, Kala RP, Bisht B, Sharma M. Antioxidant capacity and total phenolic content of Swertia chirayita (Roxb. ex Fleming) H Karst. In Uttarakhand. Int J Phar Sci Rev Res 2013;23(1):259-61.

Hajimehdipoor H, Esmaeili S, Shekarchi M, Emrarian T, Naghibi F. Investigation of some biological activities of Swertia longifolia Boiss. Res Pharm Sci 2013;8(4):253-9.

Jaishee N, Chakraborty. Evaluation of In vitro antioxidant activities of Pteris biaurita L. Int J Pharm Pharm Sci 2014;6(2):413-21.



How to Cite

Ashwini, A. M., and M. Majumdar. “QUANTIFICATION OF PHYTOCHEMICAL CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF EXACUM BICOLOR (ROXB.), AN ENDEMIC MEDICINAL PLANT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 6, June 2015, pp. 225-30, https://journals.innovareacademics.in/index.php/ijpps/article/view/5325.



Original Article(s)