GC-MS ANALYSES OF LEAF AND ROOT EXTRACTS OF DIDYMOCARPUS TOMENTOSA
Keywords:
Didymocarpus tomentosa, 5-Hydrxoymethylfurfural, Lupenone, LupeolAbstract
Objective: Didymocarpus tomentosa Wight., is a traditional medicinal plant used in the treatment of fever and skin allergy and the present study were conducted to identify the phytochemical constituents in leaf and root extracts using gas chromatography and mass spectrometry (GC-MS).
Methods: The methanolic leaf and root extracts were analyzed using Shimadzu GCMS-QP 2010 gas chromatography-mass spectrometer. The mass spectrum of GC-MS was interpreted using the database of the National Institute of Standards and Technology (NIST).
Results: The GC-MS analyses of leaf and root extracts revealed the presence of twenty six and twenty three phytochemical constituents respectively. 5-Hydrxoymethylfurfural (15.1%), cis, cis, cis-7,10,13-Hexadecatrienal (12.5%), Hexadecanoic acid (11.8%), Lupenone (29.1%), γ-Sitosterol (21.3%) and Lupeol (12.0%) were the major constituents.
Conclusion: The leaf and root extracts of D. tomentosa possess various phytochemical constituents, which are of high therapeutic values.
Â
Downloads
References
Ruan GH, Li GK. The study on the chromatographic fingerprint of Fructus xanthii by microwave assisted extraction coupled with GC-MS. J Chromatogr B 2007;850:241-8.
Weber A, Burtt BL, Vitek E. Material for revision of Didymocarpus (Gesneriaceae). Ann Naturhist Mus Wien 2000;102:441-75.
Kottaimuthu R. Ethnobotany of the valaiyans of karandamalai, Dindigul District, Tamil Nadu, India. Ethnobot Leaflets 2008;12:195-203.
Sathyavathi R, Janardhanan KJ. Folklore medicinal practices of Badaga community in Nilgiri biosphere reserve, Tamil Nadu, India. Int J Pharm Res Dev 2011;3:50-63.
Prameela J, Ramakrishnaiah H, Krishna V, Purnima A. Antioxidant and anti-inflammatory activities of Didymocarpus tomentosa Wight. Indian J Adv Plant Res 2014;1:40-5.
Hooker JD. The Flora of British India IV. L. Reeve and Co. Ltd, Kent, England; 1885.
Matthew KM. The flora of the tamilnadu carnatic. Part Two. The Rapinat Herbarium, St. Joseph's College, Tiruchirapalli, India; 1983.
Tsiapara AV, Jaakkola M, Chinou I, Graikou K, Tolonen T. Bioactivity of greek honey extracts on breast cancer (MCF-7), prostate cancer (PC-3) and endometrial cancer (Ishikawa) cells: profile analysis of extracts. Food Chem 2009;116:702-8.
Ntalli NG, Vargiu S, Menkissoglu-Spiroudi U, Caboni P. Nematicidal carboxylic acids and aldehydes from Melia azedarach Fruits. J Agric Food Chem 2010;58:11390-4.
Kitts DD, Chen XM, Jing H. Demonstration of antioxidant and anti-inflammatory bioactivities from sugar-amino acid maillard reaction products. J Agric Food Chem 2012;60:6718-27.
Zhao L, Chen J, Su J, Li L, Hu S. In vitro antioxidant and antiproliferative activities of 5-Hydroxymethylfurfural. J Agric Food Chem 2013;61:10604-11.
Pollak OJ. Reduction of blood cholesterol in man. Circulation 1953;7:702.
Balamurugan R, Duraipandiyan V, Ignacimuthu S. Antidiabetic activity of γ-sitosterol isolated from Lippia nodiflora L. in streptozotocin induced diabetic rats. Eur J Pharmacol 2011;667:410-8.
Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C. Anti-inflammatory property of n-hexadecanoic acid: structural evidence and kinetic assessment. Chem Biol Drug Des 2012;80:434-9.
Manilal A, Sujith S, Selvin J, Shakir C, Seghal Kiran G. Antibacterial activity of Falkenbergia hillebrandii (Born) from the Indian coast against human pathogens. Fyton Int J Exp Bot 2009;78:161-6.
Na M, Kim BY, Osada H, Ahn JS. Inhibition of protein tyrosine phosphatase 1B by lupeol and lupenone isolated from Sorbus commixta. J Enzyme Inhib Med Chem 2009;24:1056-9.
Mallavadhani UV, Panda AK, Rao YR. Pharmacology and chemotaxonomy of Diospyros. Phytochem 1998;49:901-51.
Cmoch P, Pakulski Z, Swaczynova J, Strnad M. Synthesis of lupane-type saponins bearing mannosyl and 3, 6-branched trimannosyl residues and their evaluation as anticancer agents. Carbohydr Res 2008;343:995-1003.
Huguet A, del Carmen Recio M, Manez S, Giner R, Rios J. Effect of triterpenoids on the inflammation induced by protein kinase C activators, neuronally acting irritants and other agents. Eur J Pharmacol 2000;410:69-81.
Argay G, Kalman A, Kapor A, Ribar B, Petrovic S. Crystal structure of a mixture of lupeol-acetate tautomers isolated from Hieracium plumulosum A. Kerner, Asteraceae. J Mol Struct 1997;435:169-79.
Agarwal RB, Rangari VD. Antiinflammatory and antiarthritic activities of lupeol and 19α-H lupeol isolated from Strobilanthus callosus and Strobilanthus ixiocephala roots. Indian J Pharm 2003;35:384-7.
Shai LJ, McGaw LJ, Aderogba MA, Mdee LK, Eloff JN. Four pentacyclic triterpenoids with antifungal and antibacterial activity from Curtisia dentata (Burm. f) C. A. Sm. leaves. J Ethnopharmacol 2008;119:238-44.
Villareal MO, Han J, Matsuyama K, Sekii Y, Smaoui A. Lupenone from Erica multiflora leaf extract stimulates melanogenesis in B16 murine melanoma cells through the inhibition of ERK1/2 activation. Planta Med 2013;79:236-43.