• Durand Dah Nouvlessounon Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, FAST, Université d’Abomey Calavi 05 BP: 1604 Cotonou, Bénin
  • Hubert Adoukonou Sagbadja Université d’Abomey Calavi 05 BP: 1604 Cotonou
  • Nafan Diarrassouba Université d’Abomey Calavi 05 BP: 1604 Cotonou
  • Haziz Sina Université d’Abomey Calavi 05 BP: 1604 Cotonou
  • PACOME A. NOUMAVO Université d’Abomey Calavi 05 BP: 1604 Cotonou
  • Farid Baba Moussa Université d’Abomey Calavi 05 BP: 1604 Cotonou
  • Adolphe Adjanohoun Université d’Abomey Calavi 05 BP: 1604 Cotonou
  • Joachim D. Gbenou Université d’Abomey Calavi 05 BP: 1604 Cotonou
  • Lamine Baba Moussa Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des sciences et techniques, Université d’Abomey-Calavi. Benin


Metabolites, Extracts, Staphylococcus, Antimicrobial, Meat products, Benin


Objective: The aim of this study was to make a phytochemical screening and measure some biological activities of Cola acuminata's seeds and leaves extracts.

Methods: The secondary metabolite was detected by the method based on coloring and precipitation differential reactions. The antibacterial and antifungal activities were assessed in vitro by the macrodillution and solid medium agar diffusion method. 2,2-diphényl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods were used to evaluate the antioxidant activity of the tested extracts of which larval cytotoxicity was studied.

Results: Our data revealed that the seeds contain more polyphenols than the leaves. The seeds extracts displayed a good antibacterial activity against both reference strains and food Staphylococcus strains. The inhibition diameters varied from 7±2.82 to 21.5±4.94 mm. The smallest Minimum Inhibitory Concentrations (MIC) on the food strains (0.08 mg/ml) is twice less than the reference strains one (0.15 mg/ml), this observation is contrary to the Minimum Bactericidal concentrations (MBC). Susceptibility of fungal strains varies according to the extracts (p = 0.0016). The seeds ethyl acetate extracts had the best antioxidant activity with DPPH and ABTS methods. The Lethal Doses (LD50) showed that no extract was toxic.

Conclusion: Cola acuminata seeds are richer than the leaves in compounds with biological activities. These two organs have interesting antimicrobial, antifungal and antioxidant activity variables depending to the extracts. The seeds have better biological activity than the leaves.



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Luximon-Ramma A, Bahorun T, Soobrattee AM, Aruoma OI. Antioxidant activities of phenolic, proanthocyanidin and flavonoid components in extracts of Acacia fistula. J Agric Food Chem 2002;50:5042–7.

Vincenot F, Saleh M, Prevost G. Les facteurs de virulence de Staphylococcus aureus. RFL 2008;38:61-9.

Ryan KJ, Ray CG. Sherris Medical Microbiology. 4th ed. McGraw Hill, New York; 2004.

Chang S, Sievert DM, Hageman JC, Boulton ML, Tenover FC, Downes FP, et al. Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene. New Engl J Med 2003;348:1342-47.

Anani K, Hudson JB, de Souza LC, Akpagana K, Towe GHN, Amason JT, et al. Investigation of medicinal plants of TOGO for antiviral and antimicrobial activities. Pharm Biol 2000;38:40-5.

Gouze ME, Oswald IP. Effets d’une mycotoxine inféodée au maïs, la fumonisine B1, sur les lymphocytes porcins. Journ Rech Porcine Fr 2001;33:277-81.

Zohra M. Etude phytochimique et activites biologiques de quelques plantes medicinales de la region nord et sud-ouest de l’algérie. Thèse de doctorat. Université Abou Bekr Belkaïd; 2013.

Boumaza I, Srinivasan S, Witt WT, Feghali-Bostwick C, Dai Y, Garcia-Ocana A, et al. Autologous bone marrow-derived rat mesenchymal stem cells promote PDX-1 and insulin expression in the islets, alter T cell cytokine pattern and preserve regulatory T cells in the periphery and induce sustained normoglycemia. J Autoimmun 2009;32:333-42.

Bagchi D, Sen CK, Bagchi M, Atalay M. Anti-angiogenic, Antioxidant, and Anti-carcinogenic properties of a novel anthocyanin-rich berry extract formula. Biochem 2004;69:75-80.

Oruma P, Prapasri P, Anadi N. Changes of antioxidant activity and total phenolic compounds during storage of selected fruits. J Food Compos Anal 2008;21:241-8.

Sina H, Baba-Moussa F, Ahoyo TA, Mousse W, Anagonou S, Gbenou JD, et al. Antibiotic susceptibility and Toxins production of Staphylococcus aureus isolated from clinical samples from Benin. Afr J Microbiol Res 2011;5:2797-808.

Sessou P, Farougou S, Azokpota P, Youssao I, Fanou B, Kaneho S, et al. In-vitro antifungal activity of essential oil of Pimenta racemosa against fungal isolates from wagashi, a traditional cheese produced in Benin. Int J Nat Appl Sci 2012;8:25-34.

Chabi Sika K, Sina H, Adoukonou-Sagbadja H, Ahoton LE, Roko GO, Saidou A, et al. Antimicrobial activity of Anacardium occidentale L. leaves and barks extracts on pathogenic bacteria. Afr J Microbiol Res 2014;8:2458-67.

Atefeibu ESI. Contribution à l’étude des tanins et de l’activité antibactérienne de Acacia nilotica var adansonii. Thèse de doctorat. Université de Cheikh Anta Diop de Dakar, Sénégal; 2002.

Sokpon N, Ouinsavi C. Utilistaion du Khaya senegalensis en medicine traditionnelle au Bénin. Rev Med Pharm Afr 2002;16:9-19.

Adjanohoun E, Adjakidjè V, Ahyi MRA, Ake Assi L, Akoegninou A, D’Almeida J, et al. Contribution aux etudes ethnobotaniques et floristiques en republique populaire du Bénin. 2nd ed. ACCT: Paris; 1989.

Akoegninou A, Van Der Burg WJ, Van Der Maesen LJG. Flore analytique du Bénin. Backhuys Publishers, Leiden, Netherlands, Wageningen University; 2006.

Lowe HIC, Watson CT, Badal S, Peart P, Toyang NJ, Bryant J. Promising efficacy of the Cola acuminata Plant: A Mini Review. Adv Biol Chem 2014;4:240-5.

Odugbemi T. Outlines and pictures of medicinal plants from nigeria. University of Lagos Press: Lagos; 2006.

Houghton DJP, Raman A. Laboratory hand book for the fractionation of natural Extracts. Sciences; 1998.

Guede-Guina F, Vangah-Mandah M, Bonga GM, de Souza C. Acticité antibactérienne d’un extrait végétale contre les germes opportunistes au cours du SIDA. Rev Med Pharm 1995;9:13-9.

Sanogo R, Diallo D, Diarra S, Ekoumou C, Bougoudogo D. Activité antibactérienne et antalgique de deux recettes traditionnelles utilisées dans le traitement des infections urinaires et la cystite au Mali. Mali Med 2006;21:18-24.

N’Guessan JD, Bidie AP, Lenta BN, Weniger B, Andre P, Guede Guina F. In vitro assays for bioactivity-guided isolation of antisalmonella and antioxidant compounds in Thonningia sanginea flowers. Afr J Biotech 2007;6:1685-9.

Attien P, Sina H, Moussaoui W, Dadié T, Chabi K, Djéni T, et al. Prevalence and antibiotic resistance of Staphylococcus strains isolated from meat products sold in Abidjan streets (Ivory Coast). Afr J Microbiol Res 2013;7:3285-93.

Bauer AW, Kirby WMM, Sherris TC, Truck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 1966;45:493-6.

Saha A, Rahman MS. Antimicrobial activity of crude extract from Calycopteris floribunsa. Bangl J Microbiol 2008;25:137-9.

Farshori NN, Al-Oqail MM, Al-Sheddi ES, Siddiqui MA, Rauf A. Antimicrobial potentiality of polyalthia longifolia seed oil against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Afr J Microbiol Res 2013;19:1977-82.

Kawsar SMA, Huq E, Nahar N. Cytotoxicity assessment of the aerial part of Macrotyloma uniflorum Lim. Int J Pharm 2008;4:297-300.

Dohou N, Yamni K, Badoc A, Douira A. Activité antifongique d’extraits de Thymelaealythroidessur Trois champignons pathogènes du riz. Bull Soc Pharm Bordeaux 2004;143:31-8.

Re R, Pellegrini N, Proteggent A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cationdecolorization assay. Free Radic Biol Med 1999;26:1231-7.

Guenne S, Ouattara N, Hilou A, Millogo JF, Nacoulma OG. Antioxidant, enzyme inhibition activities and polyphenol contents of three asteraceae species used in burkina faso traditionnally medicine. Int J Pharm Pharm Sci 2011;3:524-8.

Scherer R, Godoy HT. Antioxidant activity index (IAA) by the 2, 2-diphenyl-1-picrylhydrazyl method. Food Chem 2009;112:654-8.

Schmeda-Hirschmann G, Rodriguez JA, Theoduloz C, Astudillo L, Feresin GE, Tapia A. Free radical scavengers and antioxidants from Peumusboldus Mol. (Boldoâ€). Free Radic Res 2003;37:447-52.

Mboto CI, Udoh P. Susceptibility of Proteus mirabilis, Staphylococcus aureus and Candida albicans to Extracts of Cola acuminata. Global Adv Res J Microbiol 2014;3:078-82.

Dewole EA, Dewumi DFA, Alabi JYT, Adegoke A. Proximate and Phytochemical of Cola nitida and Cola acuminata. Park J Biol Sci 2013:1-4.

Atanda OO, Olutayo A, Mokwunye FC, Oyebanji AO, Adegunwa MO. The quality of Nigerian kola nuts. Afr J Food Sci 2011;5:904-9.

Wahab OM, Ayodele AE, Moody JO. TLC phytochemical screening in some Nigerian Loranthaceae. J Pharmacog Phytother 2010;2:64-70.

Kanoma AI, Muhammad I, Ibrahim ID, Shehu K, Maishanu HM, Isah AD. Phytochemical screening of various species of cola nut extracts for antifungal activity against phytopathogenic fungi. Am J Biol Life Sci 2014;2:18-23.

Shen H, Tang Y, Muraoka H, Washitani I. Characteristics of leaf photosyntheis and simulated individual carbon budget in Primula mutans under contrasting light and temperature conditions. J Plant Res 2008;121(2):191-200.

Sonibare MA, Soladoye MO, Esan OO, Sonibare OO. Phytochemical and antimicrobial studies of four species of cola schott & endl. (sterculiaceae). Afr J Trad CAM 2009;6:518-25.

Bruneton J. Phytochimie, plantes médicinales Les tanins. 3th ed. Editions TEC. Et DOC., Editions médicales internationales, Paris; 1999.

Thangara JHS, Adjei O, Allen BW, Portaels F. In vitro activity of ciprofloxacin, Sparfloxacin, Ofloxacin, Amikacin and Rifampicin against Ghanian isolates of Mycobacterium ulcerans. J Antimicrob Chemother 2000;45:231-3.

Adam SIY, Yahya AAN, Salih WMM, Abdelgadir WS. Antimicrobial activity of the masticatory Cola acuminata nut (Gooro). Curr Res J Biol Sci 201;3:357-62.

Nwanekwu KE, Deniyi BA, Izuka KC, Mahady G. Phytochemical and antimicrobial properties of crude n-hexane and methanol extracts of Cola acuminata nuts. Br J Pharm Res 2014;4:920-8.

Lacmata ST, Kuete V, Dzoyem JP, Tankeo SB, Teke GN, Kuiate JR, et al. Antibacterial activities of selected cameroonian plants and their synergistic effects with antibiotics against bacteria expressing MDR Phenotypes. Evid-Based Complement Alter Med 2012;2012:1-11.

Berche P, Gaillard JL, Simonet M. Les bactéries des infections humaines. Editeur: Flammarion, Médecine & Sciences, Paris; 1991.

Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R, Mulatsih W. Antioxidant activity, total phenolic, and total flavonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). Int Food Res J 2010;17:97-106.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12 Suppl 4:564-82.

Dicko HM, Hilhorst R, Gruppen H, Traore AS, Laane C, Van Berkel WJH, et al. Comparison of content in phenolic compounds, polyphenol oxidase, and peroxidase in grans of fifty sorghum varieties from Burkina Faso. J Agric Food Chem 2002;50:3180-8.

Fadlinizal MGK, Prasad N, Kong KW, Amin I. Flavonoid, hesperidine, total phenolic contents and antioxidant activities from Citrus species. Afr J Biotech 2010;9:326-30.

Topan S. Antioxidant activity of some wild edible fruits of meghalaya state in India. Adv Biol Res 2011;5:155-60.



How to Cite

Nouvlessounon, D. D., H. A. Sagbadja, N. Diarrassouba, H. Sina, P. A. NOUMAVO, F. B. Moussa, A. Adjanohoun, J. D. Gbenou, and L. B. Moussa. “ANTIMICROBIAL, ANTIOXIDANT, CYTOTOXIC ACTIVITIES AND PHYTOCHEMICAL ASSESSMENT OF COLA ACUMINATA USED IN BENIN”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 6, June 2015, pp. 102-9,



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