EVALUATION OF PHYTOCHEMICALS, ANTIOXIDANT CAPACITY AND ANTIBACTERIAL ACTION OF THE FRUIT OF THESPESIA POPULNEA (L.) SOL. EX. CORREA

Authors

  • Selina P Shah Shri C. B. Patel Research Centre, Vile Parle, Mumbai, India
  • Ashok Bhagwat Shri C. B. Patel Research Centre, Vile Parle

Keywords:

Proximate analysis, TPC, DPPH, Antimicrobial efficacy, Thespesia populnea (L) Sol ex Correa

Abstract

Objectives: To carry out proximate analysis, detect phytochemicals, determine antioxidant activity, total polyphenolic content (TPC) and antimicrobial efficacy of the methanolic extract of the fruit of Thespesia populnea (T. populnea) and further carry out Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests for susceptible microorganisms.

Methods: The following methods were used: Folin Ciocalteau method for TPC, DPPH radical scavenging assay for in vitro antioxidant activity, agar well diffusion for antimicrobial activity and broth dilution for MIC followed by MBC.

Results: The following results were obtained: TPC of 78.12±6.46 mg/g GAE; IC50 value of the radical scavenging activity: 16.29 µg/ml; antibacterial activity-cold extract: excellent to moderate against Staphylococcus aureus, Streptococcus pyogenes and Pasteurella multocida, low to no effect against Escherichia coli, Candida albicans, Pseudomonas aeruginosa and Klebsiella pneumoniae; hot extract: excellent to moderate against S. aureus, P. multocida and E. coli; relatively low to no effect on S. pyogenes, Candida albicans, Pseudomonas aeruginosa and Klebsiella pneumoniae.

MIC as well as MBC of the hot methanolic extracts of T. populnea were found to be between 0.1-2.5 mg/ml against S. aureus, E. coli and P. multocida whereas those of the cold extract against the same organisms was between 0.005-1.0 mg/ml.

Conclusion: The methanolic extract of Thespesia populnea fruit showed excellent antimicrobial activity against some dreaded pathogens besides being an antioxidant in nature. These properties of the fruit can help protect injured and/or infected tissues and hence be used to develop a novel medicinal agent.

 

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References

Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89–96.

Fang Y-Z, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition 2002;18:872–9.

Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia 2011;82:513–23.

Nijveldt RJ, Van Nood E, Van Hoorn DE, Boelens PG, Van Norren K, Van Leewen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 2001;74:418–25.

Wagener FADTG, Carels CE, Lundvig DMS. Targeting the redox balance in inflammatory skin conditions. Int J Mol Sci 2013;14:9126–67.

Ito N, Fukushima S, Hasegawa A, Shibata M, Ogiso T. Carcinogenicity of butylated hydroxy anisole in F344 rats. J Natl Cancer Inst 1983;70:343-7.

Kahl R. Synthetic antioxidants: biochemical actions and interference with radiation, toxic compounds, chemical mutagens and chemical carcinogens. Toxicology 1984;33:185-228.

Gupta VK, Sharma SK. Plants as natural antioxidants. Nat Prod Radiance 2006;5:326–34.

Narayanaswamy N, Balakrishnan KP. Evaluation of some medicinal plants for their antioxidant properties. Int J PharmTech Res 2011;3:381–5.

Geethalakshmi R, Sakravarthi C, Kritika T, Arul Kirubakaran M, Sarada DV. Evaluation of antioxidant and wound healing potentials of Sphaeranthus amaranthoides Burm. f. Biomed Res Int 2013;1-7. doi.org/10.1155/2013/607109. [Article in Press]

Ngonda F. In-vitro anti-oxidant activity and free radical scavenging potential of roots of malawian Trichodesma zeylanicumm (burm. f.). Asian J Biomed Pharm Sci 2013;3:21–5.

Paudel B, Bhattarai HD, Kim IC, Lee H, Sofronov R, Ivanova L, et al. Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from oymyakon region of the republic of sakha (Yakutia), Russia. Biol Res 2014;47:10.

Francis JK. Thespesia populnea (L.) Sol. ex Corrêa. In: Vozzo JA. editor. Tropical Tree Seed Manual. Reforestation; Nurseries and Genetics Resources; Part II, Species description. Washington DC; USDA Forest Service; 2002. p. 762-4.

Phanse MA, Patil MJ, Abbulu K. Review on pharmacological studies of Thespesia populnea linn. Int J Pharm Pharm Sci 2013;5:1–5.

Anonymous, Wealth of India. First supplementary Series, Vol-X, (Sp-w), Raw materials. Published by Niscom; 2003. p. 223-5.

Jayaweera DMA. Medicinal plants (indigenous and exotic) used in Ceylon. Part 4, National Science Council of Sri Lanka, Colombo; 1982.

Ilavarasan R, Vasudevan M, Anbazhagan S, Venkataraman S. Antioxidant activity of Thespesia populnea bark extracts against carbon tetra chloride–induced liver injury in rats. J Ethnopharmacol 2003;87:227-30.

Vasudevan M, Gunnam KK, Parle M. Antinociceptive and anti-inflammatory effects of Thespesia populnea bark extract. J Ethnopharmacol 2007;109:264–70.

Parthasarathy R, Ilavarsan R, Karrunakaran CM. Antidiabetic activity of Thespesia populnea bark and leaf extract against streptozotocin induced diabetic rats. Int J PharmTech Res 2009;1:106-9.

Moon A, Khan A, Wadher B. Antibacterial potential of Thespesia populnea (Linn.) sol. ex corr. leaves and its corresponding callus against drug resistant isolates. Indian J Nat Prod Resour 2010;1:444-9.

Ganga Rao B, Umamaheswara Rao P, Sambasiva Rao E, Mallikarjuna Rao T, Praneeth D VS. Studies on phytochemical constituents, quantification of total phenolic, alkaloid content and In-vitro anti-oxidant activity of Thespesia populnea seeds. Free Radicals Antioxid 2011;1:56–61.

Sarma DS, Venkata A, Babu S, Krishna KR. Antioxidant and anti inflammatory activities of T. populnea linn, Int J Res Pharm Chem 2011;1:674-6.

Shekshavali T, Sivakumar Hugar. Antimicrobial activity of Thespesia populnea soland. Ex correa bark extracts. Indian J Nat Prod Resour 2012;3:128-30.

Krishnamoorthy S, Adaikala Raj G, Chandrasekaran M. Antibacterial and antifungal activity of leaves of Thespesia populnea. Int J Pharm Pharm Sci 2014;6:404–11.

Quality control methods for herbal materials. Updated edition of Quality control methods for medicinal plant materials WHO; 1998.

Gokhale SB, Kokate CK. Practical Pharmacognosy. 13th ed. India: Nirali Prakashan; 2009.

Trease GE, Evans WC. Pharmacology. 11th ed. London: Bailliere, Tindall Ltd; 1978. p. 60‑75.

Khandelwal KR. Practical pharmacognosy-techniques and experiments. Pune, India: Nirali Prakashan; 1996.

Kokate CK. Practical Pharmacognosy. 4th Edition. New Delhi, Vallabh Prakashan; 2004. p. 123.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic acid-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 2002;26:1199-200.

Afolayan AJ, Jimoh FO, Sofidiya MO, Koduru S, Lewu FB. Medicinal potential of the root of Arctotis arctotoides. Pharm Biol 2007;45:486–93.

Adetutu A, Morgan W a, Corcoran O. Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria. J Ethnopharmacol 2011;133:116–9.

Valgas C, de Souza SM De, Smânia EFA, Jr AS. Screening methods to determine antibacterial activity of natural products. Brazilian J Microbiol 2007;38:369–80.

Shirwaiikar A, Vasanth Kumar A, Krishnanand BR, Sreenivasan KK. Chemical investigation and anti-hepatotoxic activity of Thespesia populnea. Int J Pharmacogn 1995;33:305-10.

Nagappa AN, Cheriyan B. Wound healing activity of the aqueous extract of Thespesia populnea fruit. Fitoterapia 2001;72:503-6.

Satyanarayana T, Saritha T, Balaji M, Ramesh A, Boini MK. Antihyperglycemic and hypoglycemic effects of Thepesia populnea fruits in normal and alloxan induced diabetes in rabbits. Saudi Pharm J 2004;12:107-11.

Vasudevan M, Gunnam KK, Parle M. Antinoceciptive and anti-inflammatory effect of hespesia. populnea bark extract, J Ethnopharmacol 2007;109:264-70.

Saravanakumar A, Venkateshwaran K, Vanitha J, Ganesh M, Vasudevan M, Sivakumar T. Evaluation of antibacterial activity of phenol and flavonoid contents of Thespesia populnea flower extracts. Pak J Pharm Sci 2009;22:282-6.

Patil PH, Patil JY, Mahale JN, Patel JB, Surana SJ. Evaluation of antiulcer activity of the terpenoid fraction from the leaves of Thespesia populnea (L) (Malvaceae) in albino rats. Res J Pharm Biol Chem Sci 2010;1:495-513.

Belhekar SN, Chaudhari PD, Saryawanshi JS, Mali KK, Pandhare RB. Antidiabetic and antihyperlipidemic effect of Thespesia populnea fruit pulp extracts on alloxan-induced diabetic rats. Indian J Pharm Sci 2013;75:217–21.

Elena DL. Pharmacognostic methods for analysis of herbal drugs, according to European pharmacopoeia. In: Basnet P. editor. Promising Pharmaceuticals. In Tech; 2012.

Soni H, Singhai AK. A recent update of botanicals for wound healing activity. Int Res J Pharm 2012;3:1–7.

Osawa T. Novel natural antioxidants for utilization in food and biological systems. In: Uritani I, Garcia VV, Mendeoza EM. editors. Postharvest biochemistry of plant food-materials in the tropics. Tokyo, Japan: Japan Scientific Societies Press; 1994. p. 241-51.

Potterat O. Antioxidants and free radical scavengers of natural origin. Curr Org Chem 1997;1:415–40.

Chandel M, Sharma U, Kumar N, Singh B, Kaur S. Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry. Asian Pac J Trop Med 2012;5:977–85.

Sun J, Liu S, Zhang C, Yu L, Bi J, Zhu F, et al. Chemical composition and antioxidant activities of Broussonetia papyrifera fruits. PLoS One 2012;7:1–8.

Mekni M, Azez R, Tekaya M, Mechri B, Hammami M. Phenolic, non-phenolic compounds and antioxidant activity of pomegranate flower, leaf and bark extracts of four Tunisian cultivars. J Med Plants Res 2013;7:1100–7.

Egea I, Sánchez-Bel P, Romojaro F, Pretel MT. Six edible wild fruits as potential antioxidant additives or nutritional supplements. Plant Foods Hum Nutr 2010;65:121–9.

Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complement Altern Med 2008;8:54.

Süntar I, Akkol EK, Nahar L, Sarker SD. Wound healing and antioxidant properties: do they coexist in plants? Free Radicals Antioxid 2012;2:1–7.

Barku VYA, Opoku-Boahen Y, Owusu-Ansah E, Mensah EF. Antioxidant activity and the estimation of total phenolic and flavonoid contents of the root extract of Amaranthus spinosus. Asian J Plant Sci Res 2013;3:69–74.

Benhaim P, Mathes S, Hunt T, Scheuenstuhl H, Benz C. Induction of neutrophil Mac-1 integrin expression and superoxide production by the medicinal plant extract gossypol. Inflammation 1994;18:443–58.

Bonham PA. Identifying and treating wound infection. J Gerontological Nursing 2009;35:12–6.

Howe CW. The problem of postoperative wound infections caused by Staphylococcus aureus. Ann Surg 1957;146:384–96.

Nakamura T, Daimon T, Mouri N, Masuda H, Sawa Y. Staphylococcus aureus and repeat bacteremia in febrile patients as early signs of sternal wound infection after cardiac surgery. J Cardiothoracic Surgery 2014;9:80.

Dryden MS. Complicated skin and soft tissue infection. J Antimicrob Chemother 2010;65 Suppl 3:35–44.

Chapple CR, Fraser AN. Pasteurella multocida wound infections—a commonly unrecognized problem in the casualty department. Injury 1986;17:410-1.

Ahmad TA, Rammah SS, Sheweita SA, Haroun M, El-Sayed LH. Development of immunization trials against Pasteurella multocida. Vaccine 2014;32:909–17.

Sies H. Oxidative Stress: Oxidants and antioxidants-(Review). Exp Physiol 1997;82:291-5.

Carocho M, Ferreira ICFR. A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol 2013;51:15–25.

Published

06-10-2015

How to Cite

Shah, S. P., and A. Bhagwat. “EVALUATION OF PHYTOCHEMICALS, ANTIOXIDANT CAPACITY AND ANTIBACTERIAL ACTION OF THE FRUIT OF THESPESIA POPULNEA (L.) SOL. EX. CORREA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 13, Oct. 2015, pp. 117-21, https://journals.innovareacademics.in/index.php/ijpps/article/view/5664.