• Mwila Mulubwa University of Zambia, Department of Pharmacy
  • Shiv Prakash University of Zambia, Department of Chemistry


Cassia abbreviata, Potency, Antibacterial, Antifungal


Objective: To evaluate the potential antimicrobial activity and relative potency of aqueous and organic extracts of Cassia abbreviata Oliv stem bark against clinical isolates of Neisseria gonorrhoeae (NG), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP) and Candida albicans (CA).

Methods: Six extracts of Cassia abbreviata Oliv stem bark were prepared as follows: Four extracts were prepared following Soxhlet extraction procedure using ethanol, water, trichloromethane (TCM) and dichloromethane (DCM)+ethanol (1:1) as solvents, respectively. Two extracts were prepared by soaking the powdered stem bark in ethanol and water, respectively, and subjected to mechanical shaking for 8 hours. Antimicrobial activities and minimum inhibitory concentrations (MIC) of extracts were evaluated in-vitro using agar well diffusion assay.

Results: All extracts except TCM were active against NG and PA with an average MIC of 78.8 µg/ml. The cold aqueous extract had a lowest MIC (46.88 µg/ml) against PA, whereas both hot and cold ethanol extracts had a lowest MIC of 46.88 µg/ml against NG. Only hot extracts (ethanol, DCM+ethanol and TCM) were active against KP with a lowest MIC of 46.88 µg/ml of TCM extract. Both extracts of cold ethanol and DCM+ethanol were active against CA with cold ethanol extract having a lower MIC of 93.75 µg/ml compared with DCM+ethanol.

Conclusion: There were variations in in-vitro antimicrobial activities (microbial growth inhibition) of Cassia abbreviata Oliv stem bark extracts which depended on the solvent used. Organic extracts were more potent against NG, PA, KP and CA compared with aqueous extracts.



Download data is not yet available.


Ndhlala AR, Amoo SO, Ncube B, Moyo M, Nair JJ, Van Staden J. In: Kuete V. editor. Antibacterial, Antifungal, and antiviral activities of african medicinal plants. Oxford: Elsevier; 2013. p. 621-59.

World Health Organization-WHO. Traditional Medicine Strategy 2002-2005. Geneva: WHO; 2002.

Fowler DG. Traditional Ila Plant remedies from Zambia. Kirkia 2002;18(1):35-48.

Phiri PS. A checklist of Zambian vascular plants. Pretoria: Sabonet; 2005.

Ndubani P, Höjer B. Traditional healers and the treatment of sexually transmitted illnesses in rural Zambia. J Ethnopharmacol 1999;67(1):15-25.

Runyoro D, Ngassapa O, Matee M, Joseph C, Moshi M. Medicinal plants used by Tanzanian traditional healers in the management of Candida infections. J Ethnopharmacol 2006;106(2):158-65.

Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998;60(1):1-8.

Izzo AA, di Carlo G, Biscardi D, de Fusso R, Mascolo N, Borrelli F, et al. Biological screening of Italian medicinal plants for antibacterial activity. Phytother Res 1995;9(4):281-6.

Maroyi A. An ethnobotanical survey of medicinal plants used by the people in Nhema communal area, Zimbabwe. J Ethnopharmacol 2011;136(2):347-54.

Kambizi L, Afolayan AJ. An ethnobotanical study of plants used for the treatment of sexually transmitted diseases (njovhera) in Guruve District, Zimbabwe. J Ethnopharmacol 2001;77(1):5-9.

Shai L, Chauke M, Magano S, Mogale A, Eloff J. Antibacterial activity of sixteen plant species from Phalaborwa, Limpopo Province, South Africa. J Med Plants Res 2013;7(26):1899-906.

Eloff JN. Quantification the bioactivity of plant extracts during screening and bioassay guided fractionation. Phytomedicine 2004;11(4):370-1.

Kolaczkowski M, Kolaczkowska A, Åšroda K, Ramalhete C, Michalak K, Mulhovo S, et al. Substrates and modulators of the multidrug transporter Cdr1p of Candida albicans in antifungal extracts of medicinal plants. Mycoses 2010;53(4):305-10.

Hamza OJ, van den Bout-van den Beukel, Carolien JP, Matee MI, Moshi MJ, Mikx FH, et al. Antifungal activity of some Tanzanian plants used traditionally for the treatment of fungal infections. J Ethnopharmacol 2006;108(1):124-32.



How to Cite

Mulubwa, M., and S. Prakash. “ANTIMICROBIAL ACTIVITY AND POTENCY OF CASSIA ABBREVIATA OLIV STEM BARK EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 6, June 2015, pp. 426-8,



Short Communication(s)