PECTINASE-MODIFIED RED GINSENG (GS-E3D) INHIBIT NF-ÎšB TRANSLOCATION AND NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDE-STIMULATED RAW 264.7 CELLS
Keywords:GS-E3D, Lipopolysaccharide (LPS), Inflammation, Nil, Mitogen-activated protein kinases (MAPKs)
Objective: Red ginseng has been used as traditional medicines and functional foods in the world, because of its health benefits. The aim of this study was to elucidate the anti-inflammatory effect and mechanism of pectinase-modified red ginseng (GS-E3D) with a cellular model of lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
Methods: To study the anti-inflammatory effect of GS-E3D, the key inflammation mediators such as nitric oxide (NO),prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), Cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-Î±), and interleukin (IL)-6 production as well as on nuclear factor kappa B (NF-ÎºB) and mitogen-activated protein kinases (MAPKs) activation, were measured by using the enzyme linked immunosorbent assay (ELISA)and Western blotting.
Results: GS-E3D potently inhibited TNF-Î± and IL-6 and also diminished NO over-production, which was accompanied by the down-regulation of iNOS expression. GS-E3D effectively suppressed LPS-induced NF-ÎºB activation through inhibiting the hyper-phosphorylation and degradation of IÎºB-Î± and phosphorylation of p38, ERK1/2 and JNK in MAPK signaling pathway.
Conclusion: GS-E3D has a potential to be as an anti-inflammatory agent for functional food or cosmetic materials targeting on the NF-ÎºB p65 and MAPKs signaling pathways.
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