• S. E. Chul Hong International Ginseng and Herb Research Institute
  • Myeong Hwan O. H. International Ginseng and Herb Research Institute
  • Hwan Lee International Ginseng and Herb Research Institute
  • Young Sik Park International Ginseng and Herb Research Institute
  • N. A. Young Kim International Ginseng and Herb Research Institute
  • S. O. Hee Park International Ginseng and Herb Research Institute
  • Jong Dae Park International Ginseng and Herb Research Institute
  • Jin Dong Jang International Ginseng and Herb Research Institute
  • Sun Hwa Kim International Ginseng and Herb Research Institute
  • Eun J. I. Kim International Ginseng and Herb Research Institute
  • M. I. Kyung Pyo International Ginseng and Herb Research Institute


GS-E3D, Lipopolysaccharide (LPS), Inflammation, Nil, Mitogen-activated protein kinases (MAPKs)


Objective: Red ginseng has been used as traditional medicines and functional foods in the world, because of its health benefits. The aim of this study was to elucidate the anti-inflammatory effect and mechanism of pectinase-modified red ginseng (GS-E3D) with a cellular model of lipopolysaccharide (LPS)-stimulated RAW264.7 cells.

Methods: To study the anti-inflammatory effect of GS-E3D, the key inflammation mediators such as nitric oxide (NO),prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), Cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α), and interleukin (IL)-6 production as well as on nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) activation, were measured by using the enzyme linked immunosorbent assay (ELISA)and Western blotting.

Results: GS-E3D potently inhibited TNF-α and IL-6 and also diminished NO over-production, which was accompanied by the down-regulation of iNOS expression. GS-E3D effectively suppressed LPS-induced NF-κB activation through inhibiting the hyper-phosphorylation and degradation of IκB-α and phosphorylation of p38, ERK1/2 and JNK in MAPK signaling pathway.

Conclusion: GS-E3D has a potential to be as an anti-inflammatory agent for functional food or cosmetic materials targeting on the NF-κB p65 and MAPKs signaling pathways.



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How to Cite

Hong, S. E. C., M. H. O. H., H. Lee, Y. S. Park, N. A. Y. Kim, S. O. H. Park, J. D. Park, J. D. Jang, S. H. Kim, E. J. I. Kim, and M. I. K. Pyo. “PECTINASE-MODIFIED RED GINSENG (GS-E3D) INHIBIT NF-ΚB TRANSLOCATION AND NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDE-STIMULATED RAW 264.7 CELLS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 9, Sept. 2015, pp. 322-6,



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