EVALUATION OF PUNICA GRANATUM FRUIT PEELS EXTRACTS FOR ITS FREE RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITY

Deepali C. Mahajan; Usha S. Satyapal; Pratima A. Tatke; Vikram A. Naharwar

Authors

Deepali C. Mahajan Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Womenâ€™s University, Santacruz (W), Mumbai 400049, India
Usha S. Satyapal Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Womenâ€™s University, Santacruz (W), Mumbai 400049, India
Pratima A. Tatke Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Womenâ€™s University, Santacruz (W), Mumbai 400049, India
Vikram A. Naharwar Amsar Goa Pvt. Ltd., Colvale, Goa 403513, India.

Keywords:

Punica granatum fruit peel, DPPH assay, NO assay, HRBC membrane, Antioxidant, Anti-inflammatory

Abstract

Objective: To evaluate free radical scavenging and anti-inflammatory activity of petroleum ether, ethyl acetate, aqueous, methanol:water and methanol extracts of Punica granatum fruit peels (PGFP) (Family: Lythraceae) by in vitro methods.

Methods: The free radical scavenging effect was studied using 1,1â€Diphenylâ€2â€Picrylhydrazyl (DPPH) and nitric oxide radical scavenging assay. Antiâ€inflammatory activity was evaluated by HRBC membrane stabilization assay.

Results: All the extracts of PGFP exhibited significant free radical scavenging effect. The methanol extract exhibited maximum significant DPPH and nitric oxide radical scavenging activity with IC₅₀value of 24.43 and 45.56Âµg/ml and maximum stabilization (86.96%) of HRBC membrane at 80 Âµg/ml among all the extracts of PGFP.

Conclusion: Methanol as an extraction solvent was found to be the best in obtaining the extract of PGFP rich in radical scavenging and anti-inflammatory phytoconsituents.

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Author Biographies

Deepali C. Mahajan, Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Womenâ€™s University, Santacruz (W), Mumbai 400049, India

Senior research Fellow

Usha S. Satyapal, Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Womenâ€™s University, Santacruz (W), Mumbai 400049, India

Senior research Fellow

Pratima A. Tatke, Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, SNDT Womenâ€™s University, Santacruz (W), Mumbai 400049, India

Department of Pharmaceutical Chemistry,

Professor of Pharmaceutical Chemistry

Vikram A. Naharwar, Amsar Goa Pvt. Ltd., Colvale, Goa 403513, India.

Director, Amsar Goa Pvt Ltd

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