PHARMACOGNOSTICAL AND PRELIMINARY PHYTOCHEMICAL INVESTIGATIONS OF AERIAL PARTS OF TRICHODESMA INDICUM R. BR.
Keywords:
Trichodesma indicum R Br Boraginaceae, UndhaphuliAbstract
Objective: This study was undertaken to carry out pharmacognostical studies and phytochemical studies on aerial parts of Trichodesma indicum R. Br belonging to the family Boraginaceae.
Methods: The aerial parts of plant leaf and stem were evaluated for pharmacognostical studies such as macroscopy, microscopy, powder study, and quantitative microscopy. The powder was evaluated for proximate analysis like ash vale, extracting value, moisture content, swelling index, elemental analysis, fluorescence analysis, and preliminary phytochemical studies.
Results: Transverse section of leaf of T. indicum R. Br. showed presence of covering trichomes with bulbous base upper and lower epidermis, collenchyma, prisms of calcium oxalate, vascular bundle and palisade cells. Surface preparation showed the presence of wavy epidermal cells, anomocytic stomata, anisocytic stomata, trichomes. Transverse section of Stem of Trichodesma indicum R. Br showed the presence of trichomes with, epidermis, hypodermis, cortex, xylem and pith. Powder study of aerial parts of T. indicum R. Br showed the presence of trichomes, xylem vessels, parenchyma, epidermal cells, fibres, calcium oxalate crystals. The powder of aerial parts was evaluated for proximate analysis such as ash value, extractive value, moisture content, total solid content, and the swelling index, which give idea about the presence of siliceous material, and amount of constituents extracted into different solvent. The elemental analysis of aerial parts showed that plant was free from heavy metal contamination i.e. arsenic, lead. The fluorescence analysis of plant powder showed that plant contains phenolic compounds. Qualitative chemical examination showed that the aerial parts of Trichodesma indicum R. Br, is credited with phytosterol, triterpenoids, tannins, phenolic compounds, carbohydrates, fixed oil, fatty acids mucilage.
Conclusion: The study reveals specific identifying characteristics for the particular crude drug which will be of significant use in identification and control to adulteration of the raw drug and can serve as a reference for any further investigations.
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References
Gupta R, Variable MG, Deshmukh RR, Chaudhary PR, Wate SR. Ethanomedicinal uses of some plants used by Gonel tribe of Bhandara district, Maharastra. Indian J Traditional Knowledge 2010;9:713-7.
Kirtikar KR, Basu BD. Indian medicinal plants. Vol. 3. Edn 2. Lalit Mohan Basu, Allahabad; 1975. p. 1672-73, 1691-5.
Dutta AC. Botany for degree students. Edn. 6. Oxford University Press, Calcutta; 1999. p. 558-60.
Shah GL. Flora of gujarat state. Edn 1. Sardar Patel University Press, Vallabh Vidyanagar; 1978. p. 455-7.
Chopra RN, Nayar SL, Chopra IC. Glossary of indian medicinal plants. Edn 1. National Institute of Science Communication, New Delhi, India; 1956. p. 247.
Chatterjee A, Prakashi SC. The treatise on indian medicinal plants. Vol. 4. National institution of science and communication and information resources; 2003. p. 216-7.
Khandelwal KR. Practical pharmacognosy techniques and experiments. Edn. 2. Nirali Prakashan, New Delhi; 2000. p. 51, 13-8.
Anonymous. Indian pharmacopoeia. Vol. 2. Ministry of Health and Family Welfare, Govt. of India, Controller of Publication: New Delhi; 1996. p. 53-4.
Mukharji PK. Quality control of herbal drugs. Edn 1. Business Horizons Pharmaceutical Publishers, New Delhi; 2002. p. 186-95.
Gupta AK. Quality standard of indian medicinal plant. Vol. 1. Indian Council of Medical Research, New Delhi; 2003. p. 236-7.
Kokate CK. Practical pharmacognosy. Edn 4. Vallabh Prakashan, Delhi; 1994. p. 148.
Sim Sk. Medicinal plant alkaloids. University of Toronto Press; 1969. p. 9.
Evans and Trease. Pharmacognosy. Edn 15. W. B. Saunders company Ltd; 2002. p. 193.
Gupta AK, Tandan N, Sharma M. Quality standard of indian medicinal plants. Vol. 3. Indian Council of Medical Research, New Delhi; 2005. p. 79-83.
Harborne JB. Phytochemical methods. Edn 2. Chapan and Hall Ltd, London; 1973. p. 42.
Feigle F. Identification of individual organic compound. In: Spot tests in organic analysis. Edn 4. Elsevier publishing company, London; 1956. p. 419-21.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Edn. 12. Nirali prakashan; 1999. p. 145-55.
Geissman A. Modern methods of plant analysis. Springer-Verlag Berlin Heidelberg. New York; 1955. p. 471.
Wagner H, Bladt S. Plant drug analysis, a thin layer chromatography atlas. Edn. 2. Springer-Verlag Berlin Heidelberg, New York; 1996. p. 342.
Stahl E. Thin layer chromatography,a laboratory hand book. Edn 2. Springer-Verlag Berlin Heidelberg, New York; 1969. p. 114.