DOSE AND STAGE SPECIFIC EFFECTS OF THYROXINE ON THE TADPOLES OF DUTTAPHRYNUS MELANOSTICTUS (ANURA: BUFONIDAE)
Keywords:
THYROXINE, DUTTAPHRYNUS MELANOSTICTUS (ANURA, BUFONIDAE)Abstract
Objective: Aim of the study was to investigate the effects of thyroxine on the pre metamorphic tadpoles of the common Asian toad, Duttaphrynus melanostictus (Anura: Bufonidae), an ideal model for understanding the role of thyroxine during vertebrate development.
Methods: Tadpoles of two developmental stages, i.e., Gosner stege 30 and 34 was exposed to 50, 100 and 200 ng/ml of thyroxine by adding the hormone to the tadpole rearing water. Toxic and teratogenic effects of thyroxine on the tadpoles were recorded. Besides, effects of thyroxine on specific activity of acid phosphatase, a lysosomal marker enzyme in the tails of the tadpoles were investigated.
Results: A Dose and stage specific toxic effect of the hormone was observed in the treated tadpoles. There was precocious metamorphosis with incomplete differentiation of limbs, deformities of body and tail in the treated tadpoles. Biochemical investigation of tails showed a dose specific elevation in the specific activity of acid phosphatase up to 2.5 fold in 50 ng/ml and 3.15 fold in 100 ng/ml treated tadpoles as compared to corresponding control tadpoles.
Conclusion: Thus, the hormone thyroxine exhibited toxic as well as teratogenic effects on the developing tadpoles and also led to dose dependent elevation in specific activity of acid phosphatase in vivo in their tails. Keywords: Acid phosphatase, Duttaphrynus melanostictus, Tail regression, Thyroxine
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Tata JR. Hormonal signaling during amphibian metamorphosis. Proc Indian Nat Sci Acad B 2003;69(5):773-90.
Tata JR. Amphibian metamorphosis as a model for the developmental actions of thyroid hormone. Mol Cell Endocrinol 2006;246(1-2):10-20.
Dodd MHI, Dodd JM. The biology of metamorphosis. In: Lofts B. editor. Physiology of Amphibia. Academic Press: New York; 1976. p. 467-599.
Nieuwkoop P, Faber J. Normal table of Xenopus laevis (Daudin). a systematical and chronological survey of the development from the fertilized egg till the end of metamorphosis. North-Holland Pub. Co. Amsterdam; 1956.
Gosner KL. A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica 1960;16:183-90.
Leloup J, Buscaglia M. La triiodothyronine, hormone de la metamorphose des amphibians. Comptes Rendus Academic Des Sci 1977;284:2261-3.
Nakajima K, Fujimoto K, Yoshio Y. Programmed cell death during amphibian metamorphosis. Cell Dev Biol 2005;16:271-80.
Gudernatsch JF. Feeding experiments on tadpoles. Arch Entwicklungsmechanik Or-ganismen 1912;35:457-83.
Weber R. Induced metamorphosis in isolated tails of Xenopus larvae. Experientia 1962;18:84-5.
Shi YB, Fu L, Hsia SC, Tomita A, Buchholz D. Thyroid hormone regulation of apoptotic tissue remodeling during anuran metamorphosis. Cell Res 2001;11(4):245-52.
Das B, Cai L, Carter MG, Piao YL, Sharov AA, Ko MSH, et al. Gene expression changes at metamorphosis induced by thyroid hormone in Xenopus laevis tadpoles. Dev Biol 2006;291:342-55.
Derby A, Jeffrey JJ, Eisen AZ. The induction of collagenase and acid phosphatase by thyroxine in resorbing tadpole gills in vitro. J Exp Zool 1979;207:391-8.
Pouyet JC, Hourdry J, Mesnard J. A histological and dynamic study of the gastric region of Discoglossus pictus larvae, cultured with or without thyroxine. J Exp Zool 1983;225:423-31.
Shi YB, Brown DS. The earliest changes in gene expression in tadpole intestine induced by thyroid hormone. J Biol Chem 1993;268(27):20312-7.
Ishizuya OA, Shimozawa A. Induction of metamorphosis by thyroid hormone in anuran small intestine cultured organotypically in vitro. Cell Dev Biol 1991;27(a): 853-7.
Ishizuya-Oka A. Amphibian organ remodeling during metamorphosis: insight into thyroid hormone-induced apoptosis. Dev Growth Differ 2011;53:202-12.
Watanabe K, Sasaki F. Ultrastructural changes in the tail muscles of anuran tadpoles during metamorphosis. Cell Tissue Res 1974;155:321-6.
Mahapatra PK, Mohanty-Hejmadi P, Chainy GBN. Changes in oxidative stress parameters and acid phosphatase activity in the pre-regressing and regressing tail of Indian Jumping frog Polypedates maculatus (Anura, Rhacophoridae). Comp Biochem Phys C 2001;130:281-8.
Mahapatra C, Mahapatra PK. Involvement of cathepsin D during tail regression in tadpoles of the common Indian tree frog, Polypedates maculatus (Anura: Rhacophoridae). Acta Histochem 2011;113:756-62.
Mahapatra C, Mahapatra PK. Immunohistochemical localization of Cathepsin D and a possible role of melanocytes during tail resorption in tadpoles of a tropical toad. J Morphol 2012;273:791–800.
Mahapatra C, Dutta SK, Mahapatra PK. Inter-populational difference in activity of acid phosphatase in the tails of the tadpoles of Duttaphrynus melanostictus (Anura: Bufonidae). Russ J Herpetol 2011;18(2):105-10.
Mahapatra PK, Jamdar SN, Mahapatra C, Dutta SK, Harikumar P. Interspecies variation in activity of lysosomal cathepsins during tail regression in the tadpoles of three sympatric anurans. Russ J Herpetol 2012;19(3):232-8.
Collins JV. Soluble acid phosphatases of the hemolymph and fat body of Calpodes ethlius (Stoll) and the control of protein storage by fat body. Can J Zool 1975;53:480-9.
Fry AE, Leius VK, Bacher BE, Kaltenbach JC. Histochemical patterns in the tadpole tail during normal and thyroxine-induced metamorphosis: Alkaline phosphatase, acid phosphatase, esterase, and aminopeptidase. Gen Comp Endocrinol 1973;21(1):16-29.
Dmytrenko GM, Kirby GS. Mechanics of tail resorption in triiodothyronine-induced metamorphosing tadpoles. J Exp Zool 1981;215:179-82.
Sasaki K, Kinoshita T, Takahama H, Watanabe K. Cytochemical studies of hydrogen peroxide production in the tadpole tail of Rana japonica during metamorphic climax. Histochem J 1988;20:99-107.
Kashiwagi A, Hanada H, Yubuki M, Kanno T, Ishisaka R, Sasaki J, et al. Thyroxine enhancement and the role of reactive oxygen species in tadpole tail apoptosis. Free Radical Biol Med 1999;26(7-8);1001-9.
Beachy CK, Surges TH, Reyes M. Effects of developmental and growth history on metamorphosis in the gray tree frog, Hyla versicolor (Amphibia, Anura). J Exp Zool 1999;283:522-30.
Beachy CK. Effects of growth history and exogenous thyroxine on size and age at metamorphosis in the toad Bufo americanus. Copeia 2001;3:829-34.
Furlow JD, Neff ES. A developmental switch by thyroid hormone: Xenopus laevis metamorphosis. Trends Endocrinol Metab 2006;17(2):38-45.
Valamparampil TT, Oommen OV. Triiodothyronine (T3) and thyroxine (T4) levels in Rana curtipes during development and metamorphosis. Indian J Exp Biol 1997;35(12):1375-7.
Divya L, Beyo RS, Sreejith P, Akbarsha MA, Oommen OV. Skeletal muscle–melanocyte association during tadpole tail resorption in a tropical frog, Clinotarsus curtipes Jerdon (Anura, Ranidea). Zoology 2010;113:175-83.
Mohanty-Hejmadi P. Care and management of amphibian embryos. Prakruti Utkal University J Sci 1977;11:81-7.
Lowry DH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem 1951;193:265-75.
Guha K, Karkkainen RR, Vanha-Perttula T. Testicular acid phosphatases of the mouse. Characterization after chromatographic fractionation. Med Biol 1979;57:52-7.
Mukai M, Obara M, Yoshizato K. Characterisation of gene of anuran cathepsin D as a metamorphosis associated enzyme. Dev Growth Differ 1995;37:463-77.
Moriya T. Prolactin induces increase in the specific gravity of salamander, Hynobius retardatus that raises adaptability to water. J Exp Zool 1982;223:83-8.
Moriya T, Dent JN. Hormonal interaction in the mechanism of migratory movement in the newt Notophthalmus viridescens. Zool Sci 1986;3:669-76.
Brown DD, Cai L. Amphibian metamorphosis. Dev Biol 2007;306(1):20-33.
Berry DL, Schwartzman RA, Brown DD. The expression pattern of thyroid hormone response genes in the tadpole tail identifies multiple resorption programs. Dev Biol 1998;203:12-23.
Taylor A, Kollros J. Stages in the normal development of Rana pipiens larvae. Anat Rec 1946;94:7-24.