IN-VITRO COMPARATIVE STUDY OF ANTI-INFLAMMATORY AND ANTI-ARTHRITIC EFFECTS OF FLEMINGIA STRICTA ROXB AND NYMPHAEA NOUCHALI LEAF
Keywords:
Flemingia stricta, Nymphaea nouchali, Anti-inflammatory, Anti-arthritic, HRBC, Protein denaturationAbstract
Objective: To evaluate the comparative study of anti-inflammatory, anti-arthritic activity of methanol extract of Flemingia stricta and Nymphaea nouchali leaf.
Methods: Human Red Blood Cell (HRBC) membrane stabilization method was evaluated for anti-inflammatory activity. Anti-denaturation method was performed by using Bovine Serum Albumin (BSA) to evaluate the anti-arthritic potential.
Results: The in vitro anti-inflammatory activity of the methanol extracts of Flemingia stricta and Nymphaea nouchali showed 81.85±0.67% (P<0.01) and 85.59±0.58% (P<0.01) of membrane stabilization at 1000µg/ml conc. and 51.85±0.49% (P<0.01) and 70.63±0.50% (P<0.01) at 31.25µg/ml respectively. All the results were compared with standard Diclofenac which showed 93.15±1.03% protection at 1000µg/ml conc. The in vitro study on both leaves also showed the presence of significant anti-arthritic activity. Here the extracts showed 70.43±1.42% (P<0.01) and 83.33±0.54% of protein denaturation at the highest conc. (1000 µg/ml) and 39.25±1.08% (P<0.01) and 38.71±0.93% (P<0.01) at the lowest conc. (31.25µg/ml), in where the standard drug displayed the 86.56±2.15% at 1000ug/ml and 51.08±1.42% at 31.25 µg/ml.
Conclusion: These results suggest that both the methanol extract of Flemingia stricta and Nymphaea nouchali possess significant anti-inflammatory and anti-arthritic activity.
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References
Hook f. Flemingia stricta Roxb. Flavour Ind 1832;3:342.
http://www.legumes-online.net/ildis/aweb/td076/td_16024. htm [Last accessed on 2015 Apr 10].
Motaleb MA, Hossain MK, Alam MK, Mamun MA, Sultana M. Commonly used medicinal herbs and shrubs by traditional herbal practitioners Glimpses from Thanchi upazila of Bandarban; 2013. p. 88-9.
Rahman MA, Uddin SB, Wilcock CC. Medicinal plants used by chakma tribe in hill tracts districts of bangladesh. Indian J Traditional Knowledge 2007;6(3):508-17.
Uddin SN. Traditional uses of ethnomedicinal plants of the chittagong hill tracts. Bangladesh National Herbarium, Bangladesh; 2006. p. 992.
Khisha T, Karim R, Chowdhury SR, Banoo R. Ethnomedical studies of chakma communities of chittagong hill tracts. Bangladesh. Bangladesh Pharm J 2012;15(1):59-67.
http://ethnobotanybd.com/index.php?action=Taxonomy&key=sci.htm [Last accessed on 2015 Apr 10].
http://www.iucnredlist.org/details/168917/0 [Last accessed on 2015 Apr 10].
http://www.mpbd.info/plants/nymphaea-nouchali.php [Last accessed on 2015 Apr 10].
Ghani A. Medicinal plants of bangladesh with chemical constituents and uses. 2nd edition. Asiatic Society of Bangladesh, Dhaka, Bangladesh; 2003.
Yusuf M. Crotalaria pallida Ait. In: Ahmed ZU, Hassan MA, Begum ZNT, Khondker M, Kabir SMH, Ahmed M, Ahmed ATA, Rahman AKA, Haque EU. eds. Encyclopedia of Flora and Fauna of Bangladesh. Vol. 8. Angiosperms: Dicotyledons (Fabaceae-Lythraceae). Asiatic Society of Bangladesh, Dhaka; 2009. p. 40-1.
Anisuzzaman M, Rahman AHMM, Harun-Or-Rashid M, Naderuzzaman ATM, Islam A KMR. An ethnobotanical study of madhupur, Tangail. J Appl Sci Res INSI Net Publication 2007;3(7):519-30.
Pavel P, Hossain ABM, Enayet. Ethnobotanical Investigation into the mandi ethnic community in Bangladesh. Bangladesh J Plant Taxonomy 2007;14(2):129-45.
Kumar V, Bhat ZA, Kumar D, Bohra P, Sheela S. In vitro anti-inflammatory activity of leaf extracts of Basella alba Linn. Var alba. Int J Drug Dev Res 2011;3(2):176-9.
Mishra NK, Bstia S, Mishra G, Chowdary KA, Patra S. Anti-arthritic activity of Glycyrrhiza glabra, Boswellia serrata and their synergistic activity in combined formulation studied in freund’s adjuvant induced arthritic rats. J Pharm Educ Res 2011;2(2):92-8.
Lavanya R, Maheshwari SU, Harish G, Raj JB, Kamali S, Hemamalani D, et al. Investigation of In-vitro anti-Inflammatory, anti-platelet and antiarthritic activities in the leaves of Anisomeles malabarica Linn. Res J Pharm Biol Chem Sci 2010;1(4):746-52.
Singh M, Soni P, Upmanyu N, Shivhare Y. In-vitro anti-arthritic activity of Manilkara zapota Linn. Asian J Pharm Technol 2011;1(4):123-4.
Chippada SC, Vangalapati M. Antioxidant, an anti-inflammatory and anti-arthritic activity of Centella asiatica extracts. J Chem Biol Phys Sci 2011;1(2):260-9.
Chou CT. The anti-inflammatory effect of Tripterygium wilfordii Hook F on adjuvant-induced paw edema in rats and inflammatory mediators release. Phytother Res 1997;11:152-4.
Murugasan N, Vember S, Damodharan C. Studies on erythrocyte membrane IV. In vitro haemolytic activity of Oleander extract. Toxicol Lett 1981;8:33-8.
Vadivu R, Lakshmi KS. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchinensis (Lour) Moore ssp Laurina. Bangladesh J Pharmacol 2008;3:121-4.
Shravan KN, Kishore G, Siva KG, Sindhu PES. In vitro anti-inflammatory and anti-arthritic activity of leaves of Physalis Angulata L. Int J Pharm Ind Res 2011;1(3):211-3