UTILIZATION OF UNSERVICEABLE STRAWBERRIES FOR PRODUCTION OF ELLAGIC ACID AND ITS ENHANCEMENT BY ASPERGILLUS NIGER
Abstract
Objective: Use of Aspergillus Niger (616) for the fabrication of ellagic acid from unserviceable strawberry as a substrate which is readily and chiefly available from Agro-industries and farm waste.
Methods: The ellagic acid content was determined by HPLC method which shows a higher concentration of ellagic acid (143.085+1.669 ppm) after fermentation of 96 h at 35 °C. DPPH assay was carried out for antioxidant activity of ellagic acid.
Results: Results designated that ellagic acid has higher antioxidant activity as compared with ascorbic acid.
Conclusion: Existing study exposes that this agro waste and farm waste can be used at commercially for the production of ellagic acid which has enormous medicinal properties.
Keywords: Ellagic acid, Raw strawberry, DPPH assay, Aspergillus Niger, Fermentation, and HPLC
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References
Eastwood MA. Interaction of dietary antioxidants in vivo: how fruit and vegetables prevent disease. Quart J Med 1999;92:527–30.
Maas JL, Galletta GJ, Stoner GD. Ellagic acid, an anticarcinogen in fruits, especially in strawberries. Hort Sci 1990;26:10-4.
Urquiaga I, Leighton F. Plant polyphenol antioxidants and oxidative stress. Biol Res 2000;33:55–64.
Vattem DA, Shetty K. Biological functionality of ellagic acid: a review. J Food Biochem 2005;29:234–66.
Olsson ME, Gustavsson KE, Andersson S, Nilsson A, Duan RD. Inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidants levels. J Agric Food Chem 2004;52:7264–71.
Clifford MN, Scalbert A. Ellagitannins-nature, occurrence and dietary burden. J Sci Food Agric 2000;80:1118-25.
Barrajon Catala, Enrique, Fernadez Arroyo, Salvado, Roldan, Cristina Guillen, et al. A systemic study of the polyphenolic composition of aqueous extracts deriving from several cistus genus species Evolutionary relationship. Phytochem Anal 2011;22:303-12.
Dehshahri FS, Wink MS, Afsharypuor G, Asghari, Mohagheghzadeh A. Antioxidant activity of methanolic leaf extract of Moringa peregrine (Forssk.) Res Pharm Sci 2007;7:111-8.
Perchellet JP, Gali H, Perchellet EM, Laks PE, Bothari V, Hemingway RW. Anti-tumor promoting effects of gallotannis, ellagitannins and flavonoids in mouse skin in vivo. In: Food phytochemicals for cancer prevention 1: Fruits and vegetables. Washington, DC: American Chemical Society Symposium Series; 1994;546:303–27.
Mandal S, Stoner GD. Inhibition of N-nitrosobenzyl-methylamine-induced esophageal tumorigenesis in rats by ellagic acid. Carcinogen 1990;11:55–61.
Paranthaman RS, Kumaravel, Singaravadivel K. Development of bioprocess technology for the production of the bioactive compound, Ellagic acid from tea extract. Department Food Safety Quality Testing Indian Institute Crop Processing Technol 2013;472:90-109.
Aguilera-Carbó A, Augur C, Prado-Barragan LA, Favela-Torres E, Aguilar CN. Microbial production of ellagic acid and biodegradation of ellagitannins. Appl Microbiol Biotechnol 2008;78:189-99.
Aguilera-Carbó A, Hernández JS, Augur C, Prado-Barragan LA, Favela-Torres E, Aguilar CN. Ellagic acid production of creosote bush ellagitannins by Aspergillus niger in solid state culture. Food Bioprocess Technol 2009;2:208-12.
Cuccioloni M, Mozzicafreddo M, Sparapani L, Spina M, Eleuteri AM, Fioretti E, et al. Pomagranate fruit components modulate human thrombin. Fitoterapia 2009;80:301-5.
Izhaki I. Influence of non-protein nitrogen on the estimation of protein from total nitrogen in fleshy fruits. J Chem Ecol 1993;19:2605-15.
Sarikaya A, Ladisch MR. Solid state fermentation of lignocellulosic plant residues from Brassica napus by Pleurotus ostreatus. Appl Biochem Biotechnol 1999;82:1-15.
Crozier A, Jagnath IB, Clifford MN. Dietary phenolics: chemistry, bioavailability and effects on health. Nat Prod Rep 2009;26:1001-43.
Pfundstein B EI, Desouky SK, Hull WE, Haubner R, Erben G, Owen RW. Polyphenolic compounds in the fruits of Egyptian medicinal plants: characterization, quantification, and determination of antioxidant capacities. Phytochem 2010;71:1132-48.
Zafrilla P, Ferreres F, Thomas-Barberan FA. Effects of processing and storage on antioxidant ellagic acid derivatives and flavonoids of red raspberry jams. Agric Food Chem 2001;49:3651-5.
David J Williams, David Edwards, Sharon Pun, Mridusmita Chaliha, Yasmina Sultanbawa. Profiling ellagic acid content†the importance of form and ascorbic acid levels. Int Food Res 2014;66:100-6.