DIFFERENTIAL EXPRESSION OF NITRIC OXIDE SYNTHASES IN THE SKIN TISSUE OF P-PHENYLENEDIAMINE-TREATED MICE
Keywords:
Nitric oxide synthase, p-phenylenediamine, Dorsal skin, Ear, SensitizationAbstract
Objective: p-Phenylenediamine (PPD) is a possible contact sensitizer in skin tissues; however, data are lacking regarding its specific effects on nitric oxide synthase (NOS) expression during the sensitization phase. The purpose of this study was to investigate nNOS and iNOS expression in the skin tissue of PPD-treated mice.
Methods: BALB/c mice were dermally exposed to PPD, at a dose of 10 or 50 mg/kg, on three occasions. The ear and dorsal skin tissues were then isolated from PPD-treated mice and vehicle-treated controls. Western blot analyses were performed on samples derived from the skin tissues.
Results: The dorsal skin tissues of PPD-treated BALB/c mice showed significantly increased levels of iNOS. However, nNOS expression in dorsal skin, and nNOS and iNOS expression in the ear, was not significantly altered in PPD-treated skin tissues compared to controls.
Conclusion: Because enhanced expression of iNOS may contribute to inflammation in allergic contact dermatitis, our data suggest that increased levels of iNOS may be involved in early immunological responses to PPD-induced pathogenesis in dorsal skin.
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References
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