BARLERIA MONTANA WIGHT AND NEES–A PROMISING NATURAL ANTI-INFLAMMATORY AGENT AGAINST FORMALIN INDUCED INFLAMMATION
Keywords:
Barleria montana, Formalin, Inflammation, IndomethacinAbstract
Objective: To evaluate the anti-inflammatory activity of the ethanolic leaf extract of Barleria montana (B. montana) against formalin induced inflammation.
Methods: Male albino wistar rats were pretreated with oral doses of 100 mg, 200 mg and 300 mg of the extract for 30 days and the animals received a single dose of sub plantar injection of formalin (0.1 ml/kg body weight (bw.)) Indomethacin (25 mg/kg bw.) was used as the standard drug. The effect of the extract on paw thickness, biochemical and hematological parameters was investigated along with histopathological studies.
Results: The results revealed that the extract exhibited an effective dose dependent activity against formalin induced inflammation with a maximum activity at a dosage of 300 mg/kg bw. which was comparable with the standard drug.
Conclusion: The results signify promising activity of ethanolic leaf extract of B. montana against formalin induced inflammation in albino rats. The extract was also found to exhibit appreciable antioxidant activity.
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Sosa S, Altinie G, Politi M, Braca A, Morelli I, Loggia RD. Extracts and constituents of Lavandula multifida with topical anti-inflammatory activity. Phytomedicine 2005;12:271-7.
Libby P. Inflammation in atherosclerosis. Nature 2002;420:868–74.
Dandona P, Aljada A, Bandyopadhyay A. Inflammation: the link between insulin resistance, obesity and diabetes. Trends Immunol 2004;25:4–7.
Lisa Coussens M, Zena Werb Z. Inflammation and Cancer. Nature 2002;420:860-7.
Jing Liu J, Jianbo Wan J, Cheng Wei He C. Rationale for the use of natural anti-inflammatory agents in cancer chemotherapy. North Am J Med Sci 2010;3:160-6.
Brunton LL, Lazo JS, Parker LK. The Pharmacological Basis of Therapeutics. London: Medical publishers; 2006. p. 671-12.
Donihi AC, Raval Saul D, Korytkowsk MT, Devita MA. Prevalence and predictors of corticosteroid-related hyperglycemia in hospitalized patients. Endocr Pract 2006;12:358-62.
Wallace JL. Nonsteroidal anti-inflammatory drugs and gastroenteropathy: the second hundred years. Gastroenterology 1997;112:1000-16.
Shah BN, Seth AK, Maheshwari KM. A Review on medicinal plants as a source of anti-inflammatory agents. Res J Med Plant 2011;5:101-15.
Shanaz Banu S, Arunachalam G, Jayaveeera KN, Josephine Leno Jenita J, Ayesha Siddiqua A. Pharmacognostic studies of Barleria montana DUN. Leaves. World J Pharm Res 2012;1:332-9.
Joseph SM, George MC, Nair JR. Effect of feeding cuttlefish liver oil on immune function, inflammatory response and platelet aggregation in rats. Curr Sci 2005;88:507-10.
Duffy JC, Dearden JC, Rostron C. Design, Synthesis and biological testing of a novel series of anti-inflammatory drugs. J Pharm Pharmacol 2001;53:1505-14.
Beutler E, Duron C, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882-8.
Rotruck JT, Pope AL, Ganther H, Swanson AB, Hafeman DG, Hoeksira WG. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973;179:588-90.
Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for SOD. J Biol Chem 1972;247(5, Suppl 10):3170-5.
Ohkawa H, Ohishi N, Yagi K. Assay of lipid peroxides in animal tissues for thiobarbituric acid reaction. Annu Biochem 1979;95:351-8.
King J. In: Practical Clinical Enzymology, Princeton MJ (Fol) Van D Nostrand Company, London; 1965. p. 363.
Okinaka S, Kumagi Ebashi S, Sugita H, Morno H, Toyokura Y. Serum Creatine phosphokinase activity in progressive muscular dystrophy and neuromuscular disease. Arch Neurol 1961;4:520-5.
Armour D, Blood FR, Belden DA. The manual for laboratory work in mammalian Physiology. 3rd ed. Chicago: The university of Chicago press; 1964. p. 4-6.
Dacie J, Lewis S. Practical haematology. London: Churchill Livingstone; 1995.
Sujai Suneetha S. Histopathological Techniques, in Handbook of CMAI Medical Laboratory Technology by Robert H Carman. Christian Med Assoc India 1993;24:508-41.
Young H, Lu Y, Cheng H, Hsieh W, Lia J, Peng W. Analgesic and anti-inflammatory activities of Gingerol. J Ethanopharmacol 2005;96(1, Suppl 2):207-10.
Hassa MQ, Hadi RA, Al-Rawi ZS. The glutathione defense system in the pathogenesis of rheumatoid arthritis. J Appl Toxicol 2001;21:69-73.
Goel RK, Sairam K, Rao CH, Raman A. Role of gastric antioxidant and anti Helicobacter pylori activities in the anti ulcerogenic activity of banana. Indian J Exp Biol 2001;39:719-22.
Agnel Arul John N, Shobana G. Anti-inflammatory activity of Talinum fruticosum L. on formalin induced paw edema in albino rats. J Appl Pharm Sci 2012;2:123-7.
Hull MA, Gardner SH, Hawcroft G. Activity of the non-steroidal anti-inflammatory drug indomethacin against colorectal cancer. Cancer Treat Rev 2003;29:309–20.
Habila JD, Bello IA, Dzikwi AA, Musa H, Abubakar N. Total phenolics and antioxidant activity of Tridax procumbens Linn. Afr J Pharm Pharmacol 2010;4:123–6.
Saraswathi CD, Wagh SP, Kunal PW. Anti-arthritic activity of Morinda citrifolia L. fruit juice in Complete Freund’s adjuvant induced arthritic rats. J Pharm Res 2012;5:1236-9.
Apple FS, Murakami M, Panteghini M. International survey on the use of cardiac markers. Clin Chem 2001;47:587–8.