IN VITRO FREE RADICAL SCAVENGING AND ANTICANCER POTENTIAL OF ARISTOLOCHIA INDICA L. AGAINST MCF-7 CELL LINE
Keywords:
Aristolochia indica L, Ehrlich Ascites Carcinoma, Free radicals, AntioxidantAbstract
Objective: To investigate the in vitro free radical scavenging and anticancer potential of Aristolochia indica L. leaves and stem against MCF-7 cell line.
Methods: Phytochemicals were analysed in chloroform (leaves) and aqueous extracts (stem) of the Aristolochia indica by using standard methods. In vitro antioxidant studies were carried out for the chloroform and aqueous extracts of the Aristolochia indica using various free radical models such a DPPH, Reducing power assay, hydrogen peroxide (H2O2) scavenging,. In vitro cytotoxic assay such as MTT assays were carried out against MCF-7 cell line.
Results: Preliminary phytochemical screening revealed the presence of flavonoids, tannins, glycosides, phenol, saponins, in chloroform extract of leaves and aqueous extract of stems. The results revealed that the chloroform extract has significant antioxidant potential than aqueous extract. The result revealed that the chloroform extracts of Aristolochia indica L showed pronounced anticancer activity against Ehrlich Ascites Carcinoma (MCF-7) cell line than ethanol extract.
Conclusion: The result of the present study concluded that the chloroform extract of Aristolochia indica L has significant antioxidant and anticancer activity then the aqueous extract. The potential antioxidant and anticancer activity of Aristolochia indica L might be due to the presence of phytochemicals.
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Patil M, Patki P, Kamath HV, Patwardhan B. cancer activity of Tinospora cordifolia (Willd) Miers. Indian Drugs 1997;34:211-5.
Sariego J. "Breast cancer in the young patient". Am Surg 2010;76(12):1397–401.
Goldhirsch A, Ingle JN, Gelber RD, Coates AS, Thürlimann B, Senn HJ. Thresholds for therapies: highlights of the St gallen international expert consensus on the primary therapy of early breast cancer. Ann Oncol 2009;20(8):1319–29.
Soule HD, Vazquez J, Long A, Alberts, Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst 1973;51(5):1490-16.
Fabricant D, Farnsworth N. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 2001;109(1):69-75.
Alviano D, Alviano A. Biochemical evaluation of extracts from caralluma tuberculata against hormone dependent breast cancer cells. J Pharm Pharmacol 2009;183:308-15.
Sakarkar D, Deshmukh V. Ethnopharmacological review of traditional medicinal; 2011.
Sanjay Kumar Karan, Sagar Kumar Mishra, Dilipkumar Pal, Arijit Mondal. Isolation of β-sitosterol and evaluation of antidiabetic activity of Aristolochia indica in alloxan-induced diabetic mice with a reference to in-vitro antioxidant activity. J Med Plants Res 2012;6(7):1219-23.
Cordell G. Natural products in drug discovery–creating a new vision; 2002.
Kanjilal PB, Kotoky R, Couladis M. Chemical composition of the stem oil of Aristolochia indica L. J Essent Oil Res 2009;21:1-2.
Achari B, Bandopadhyay S, Saha CR, Pakrashi SC. A phenanthroid lactone, steroid and lignans from aristolochia indica heterocycles. Chem Pharm Bull 1983;20:771-4.
Chopra RN, Nagar SL, Chopra IC. Glossary of medicinal plants. 7thedition. National institute of science communication and information resources; 2006. p. 24.
Masud Rana AYKM, Khanam JA. Aristolochia indica Whole Plant Extract as an Antineoplastic Agent. J Med Sci 2002;2(4):202-5.
Mavundza EJ, Tshikalange TE, Lall N, Hussein AA, Mudau FN, Meyer JJM. Antioxidant activity and cytotoxicity effect of flavonoids isolated from Athrixia phylicoides. J Med Plant Res 2010;4:2584-7.
Parekh J, Chanda S. Phytochemical screening of some plants from western region of India. Plant Arch 2008;8:657-62.
Yildirim A, Mavi A, Kara AA. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 2001;9:4083-9.
Usha Venkatachalam, Suriyavathana Muthukrishnan. Free radical scavenging activity of ethanolic extract of Desmodium Gangeticum. J Acute Med 2001;2:36-42.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Didry N, Dubreul L, Trotin F, Pinkas M. Antimicrobial activity of the aerial parts of the drosera pellagra smith on oral bacteria. J Ethnopharmacol 1998;60:91-6.
Iwu MM, Duncan AR, okunji CO. New antimicrobials of plant origin. In: prospective on New Crops and New Uses, Janick J. Ed. ASHS press; 1999. p. 457-62.
Pengelly A, Allen, Unwin. The constituents of medicinal plants, Australia. diseases. Curr Pharm Biotechnol 2004;10:106-21.
Balandrin J, Nair R, Chanda S. preliminary screening of some folklore medicinal plant from western India for potential antimicrobial activity. Indian J Pharmacol 1985;37:408-9.
Halliwell B. Antioxidant and human disease-A general introduction. Nutr Rev 1994;55:522-44.
Lo liger J. The use of antioxidants in food. In Aruoma OI, Halliwell B. Free radicals and food additives. Taylor and Francis, London. Afr J Biotechnol 1991;7(4):129–50.
Arthan D, Svasti J, Kittakoo P, Pittayakhachonwut D, Tanticharoen M. The btaranonth Y antiviral isoflavonoid sulphate and steroidal glycosides from the fruit of solanum torvum. Phytochemistry 2002;59:459-63.
Velavan Sivanandham. Free radicals in health and diseases-a mini review. Pharmacologyonline Newsletter; 2011. p. 1062-77.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total Flavonoid content in Propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
Gorelik S, Legumsky M, Kohen R, Kanner J. A novel function of red wine polyphenols in humans: prevention of absorption of cytotoxic lipid peroxidation products. FASEB J 2008;22:41-6.
Gajula D, Verghese M, Boateng J, Walker LT, Shackelford L, Mentreddy SR, et al. Determination of total phenolics, flavonoids, and antioxidants and chemopreventive potential of basil. Int J Cancer Res 2009;5:130-43.
Duh PD, Tu YY, Yen GC. Antioxidant activity of the aqueous extract of harng Jyur (Chrysanthemum morifolium Ramat). Lebensmittel-Wissenschaft Technologie 1999;32:269-77.
El-Seedi HR, Nishiyama S. Chemistry of bioflavonoids. Indian J Pharm Edu 2002;36:191-4.
Brown. Phytochemical screening and nutrient-antinutrient composition of selected tropical green vegetables. Afr J Biotechnol 1998;4:4997-501.
Rietjens IM, Boersma MG, Van der Woude H, Jeurissen SM, Schutte ME, Alink GM. Flavonoids and alkenylbenzenes: mechanisms of mutagenic action and carcinogenic risk. Mutat Res 2005;574:124-38.
Scambia G, Ranelletti FO, Panici PB. Quercetin potentiates the effect of adriamycin in a multidrug-resistant MCF-7 human breast cancer cell line: P-glycoprotein as a possible target. Cancer Chemother Pharmacol 1994;34:459-64.
Valko V, Fickova M, Pravdova E, Nagy M, Grancai D, Czigle S. Cytotoxicity of water extracts from Leaves and branches of Philadelphus coronaries L. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2006;150(1):71-3.
Konishi T, Shimada Y, Nagao T, Okabe H, Konoshima T. Antiproliferative 37. sesquiterpene lactones from the roots of Inula helenium. Biol Pharm Bull 2002;25:1370-2.
Blois MS. Antioxidant determination by the use of a stable free radical. Nature 2002;26:1199-200.
Hamelers IH, Van Schaik RF, Sussenbach JS, Steenbergh PH. 17 beta-estradiol responsiveness of MCF-7 Laboratory Strains is dependent on an autocrine signal activating the IGF type I receptor. Cancer Cell Int 2003;3(1):10-20.
Kulbe H, Hagemann T, Szlosarek PW, Balkwill FR, Wilson JL. The inflammatory cytokine tumor necrosis factor-alpha regulates Chemokine receptor expression on ovarian cancer cells. Cancer Res 2005;65(22):10355-62.