SIMULTANEOUS DETERMINATION OF CARVEDILOL AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORM BY FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRY
Keywords:
Carvedilol, Hydrochlorothiazide, UV visible spectrophotometry, Method Validation, First order derivative methodAbstract
Objective: To develop an accurate, precise and linear UV spectrophotometry method for simultaneous determination of Carvedilol and Hydrochlorothiazide in Co-Dilatrol® tablet and validate as per ICH guidelines.
Methods: Derivative spectrophotometric methods: The amplitudes in simultaneous determination of the first order derivative of the resultant spectra at 301 nm and at 278 nm were selected to find out Carvedilol and Hydrochlorothiazide respectively in its tablet dosage form by using methanol as a solvent.
Results: The linearity was found to be 5-25 μg/ml for Carvedilol and Hydrochlorothiazide. The mean % recoveries were found to be 101.13% and 99.02% for simultaneous determination of first order derivatives method of Carvedilol and Hydrochlorothiazide. For Intraday precision, Inter day precision % RSD was found to be 0.0082, 0.6304 and 0.0096, 0.6354 for Carvedilol and 0.0085, 0.6257 and 0.0083, 0.6398 for Hydrochlorothiazide respectively. Limit of Detection and Quantitation was found to be 0.7852μg/ml and 2.2539μg/ml for Carvedilol and 0.7859μg/ml and 2.3571μg/ml for Hydrochlorothiazide. Assay results of market formulation were found to be 100.89% for simultaneous determination of the first order derivatives method of Carvedilol and Hydrochlorothiazide. The proposed method has been validated as per ICH guidelines and successfully applied to the simultaneous determination of the first order derivatives method of Carvedilol and Hydrochlorothiazide in its tablet dosage form.
Conclusion: A simple, accurate, precise, linear and rapid UV spectrophotometry method was developed for simultaneous determination of Carvedilol and Hydrochlorothiazide in Co-Dilatrol® tablet and validated as per ICH guidelines. Hence it can be used for the routine analysis of Carvedilol and Hydrochlorothiazide in tablets in various pharmaceutical industries.
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