HYPERICUM PERFORATUM IMPROVE MEMORY AND LEARNING IN ALZHEIMER'S MODEL: (EXPERIMENTAL STUDY IN MICE)
Keywords:
Neurotoxicity, Nil, Phytotherapy, Hypericum perforatum, Neuroprotective, MiceAbstract
Objective: The aim of this study, we based on protective and antioxidant efficiency of Hypericum perforatum that shows a wide range of beneficial effect in vitro and in vivo.
Methods: The in vitro antioxidant activity of the extract was assessed by using several antioxidant tests. The cytotoxic activity of Hypericum perforatum was also determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide viability assay on ordinary used cell lines. In vivo experiments in Swiss mice were determined by performing behavioral, memory tests and histological study. According to tests results, H. perforatum may be relevant to the treatment of cognitive disorders.
Results: The results of chemical analysis showed a hight level of hyperforin and quercitin that had an important antioxidant activity proved in vitro with the 2, 2-diphenyl-1-picrylhydrazyl, Anti-lactoperoxidase and superoxide dismutases; this antioxidant activity was confirmed in vivo after the non-toxic results by means of improvement in behavioral and memory than the reducing shrunken in pyramidal cells of mice brains.
Conclusion: The present study suggests that Hypericum perforatum modulate the oxidative stress and be involved in the protective effect against oxidative damage and neurodegenerative diseases in mice.
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Francis PT, Palmer AM, Snape M, Wilcock GK. The cholinergic hypothesis pharmacology, biochemistry and behavior of Alzheimer’s disease: a review of progress. J Neurol Neurosurg Psychiatry 1999;66:137–47.
Adams M, Gmunder F, Hamburger M. Plants traditionally used in age related brain disorders a survey of the ethno-botanical literature. J Ethnopharm 2007;113:363–81.
Reddy PH, McWeeney S. Mapping cellular transcriptosomes in autopsied Alzheimer’s disease subjects and relevant mouse models. Neurobiol Aging 2005;27:1060-77.
Selkoe DJ. Alzheimer’s disease: genes, proteins, and therapy. Physiol Rev 2001;81:741–66.
Bossy-Wetzel E, Schwarzenbacher R, Lipton SA. Molecular pathways to neurodegeneration. Nat Med 2004;10:2–9.
Mattson MP. Pathways towards and away from Alzheimer's disease. Nature 2004;430:631–9.
Ben-Yoseph O, Boxer PA, Ross BD. Assessment of the role of the glutathione and pentose phosphate pathways in the protection of primary cerebrocortical cultures from oxidative stress. J Neurochem 1996;66:2329–37.
Behl C, Mosmann B. Antioxidant neuroprotection in Alzheimer’s disease as preventive and therapeutic approach. Free Radical Biol Med 2002;33:182–91.
Foy CJ, Passmore AP, Vahidassr MD, Young IS, Lawson JT. Plasma chain-breaking antioxidants in Alzheimer’s disease, vascular dementia, and Parkinson’s disease QJM. J Assoc Physicians India 1999;92:39–45.
Gardner PT, White TAC, McPhail DB, Duthie GG. The relative contributions of vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices. Food Chem 2000;68:471–4.
Halliwell B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radical Res 1996;25:57–74.
Halliwell B. Establishing the significance and optimal intake of dietary antioxidants: the biomarker concept. Nutr Res 1999;57:104–13.
Youdim KA, Spencer JP, Schroeter H, Rice-Evans C. Dietary flavonoids as potential neuroprotectants. Biol Chem 2002;383:503–19.
Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49:5165–70.
Wills RBH, Bone K, Morgan M. Herbal products: active constituents, modes of action and quality control. Nutr Res Rev 2000;13:47–77.
Gaedcke F. St John’s wort herb extracts: manufacturing, standardization and characterization. Hypericum, medicinal and aromatic plants. In: E Ernst. Ed. London: Taylor and Francis Group; 2003;31:49–64.
Butterweck V. Mechanism of action of St. John’s wort in depression: what is known? CNS Drugs 2003;17:539–62.
Di Carlo G, Borrelli F, Ernst E, Izzo AA, St John’s. Wort: prozac from the plant kingdom. Trends Pharmacol Sci 2001;22:292–7.
Di Carlo A, Baldereschi M, Amaducci L. Incidence of dementia, Alzheimer's disease and vascular dementia in Italy. The ILSA Study. J Am Geriatr Soc 2002;50:41-8.
Agostinis P, Vantieghem A, Merlevede W, de Witte PAM. Hypericin in cancer treatment: more light on the way. Int J Biochem Cell Biol 2002;34:221-41.
Prince AM, Pascual D, Meruelo D, Liebes L, Mazur Y, Dubovi, et al. Influence of prenylated and nonprenylated flavonoids on liver microsomal lipid peroxidation and oxidative injury in rat hepatocytes. Food Chem Toxicol 2001;39:437–45.
Zou WQ, Puoti G, Xiao X, Yuan J, Qing L, Cali I, et al. Variably protease-sensitive prionopathy: a new sporadic disease of the prion protein. Ann Neurol 2010;68:162-72.
Bombardelli E, Morazzoni P. Hypericum perforatum. Fitoterapia LXVI 1995;1:43-68.
Hostettmann K, Marston A, Wolfender JL. Strategy in the search for new lead compounds and drugs from plants. Chimia 2005;59:291-4.
Trovato A, E Raneri, M Kouladis, O Tzakou, M Taviano, E Galati. Anti-inflammatory and analgesic activity of hypericum empetrifolium willd. (Guttiferae). Farmaco 2001;56:455–7.
Rabanal R, C Bonkanka, M Hernández-Pérez, C Sánchez-Mateo. Analgesic and topical anti-inflammatory activity of Hypericum canariense L. and Hypericum glandulosum. J Ethnopharmacol 2005;96:591-6.
Slinkard K, Singleton VL. Total phenol analyses: automation and comparison with manual methods. Am J Enol Vitic 1977;28:49-55.
Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). J Food Chem 2005;89:569–75.
Duh PD, Tu YY, Yen GC. Antioxidants activity of aqueous extract of Harnjyur (Chrysanthemum morifolium Ramat). Lebensmwiss Technol 1999;32:269-77.
Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25-30.
Buege JA, Aust SD. Lipid peroxidation. Methods Enzymol 1978;51:302-10.
Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 1972;46: 849-54.
Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986;103:413-9.
Alley MC, Scudiere DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, et al. Feasibility of drug screening with panels of human tumor cell lines. Cancer Res 1988;48:4827-33.
Crockett SL, Schaneberg B, Khan I. Phytochemical profiling of new and old world hypericum (St. John’s wort) species. Phytochem Anal 2005;16:479–85.
Crockett SL, Eberhardt M, Kunert O, Schuhly W. Hypericum species in the paramos of central and south america: a special focus upon H. irazuense Kuntze. Phytochem Rev 2010;9:255–69.
Mártonfi P, Repcák M, Zanvit P. Secondary metabolites variation in Hypericum maculatum and its relatives. Biochem Syst Ecol 2006;34:56–9.
Nahrstedt A, Butterweck V. Lessons learned from herbal medicinal products: the example of St. John’s wort. J Nat Prod 2010;73:1015–21.
Röder C, Schaefer M, Leucht S. Meta-analyse zu wirksamkeit und verträglichkeit der behandlung der leichten und mittelschweren depression mit johanniskraut. Fortschr Neurol Psychiatr 2004;72:330–43.
Hölzl J, Petersen M. Chemical constituents of hypericum ssp. hypericum–the genus hypericum. In: E Ernst. Ed. London, Taylor and Francis New York; 2003.
Beerhues L. Biosynthesis of the active Hypericum perforatum constituents. Med Aromatic Plant Sci Biotechnol 2011;5:70–7.
Butterweck V, Schmidt M. St. John’s wort: role of active compounds for its mechanism of action and efficacy. Wien Med Wochenschr 2007;157:356–61.
Linde K, Berner MM, Kriston L. St. John’s wort for major depression. Cochrane Database Systematic Rev 2008;4:1–143.
Linde K. St John’s wort–an overview. Forsch Komp Klas Nat 2009;16:146–55.
Roth L. Hypericum, Hypericin: Botanik, Inhaltsstoffe, Wirkung. Landsberg/lech: ecomed; 1990.
Avato P. A survey on the hypericum genus: secondary metabolites and bioactivity. Stud Nat Prod Chem 2005;30:603–34.
Müller WE. St John’s wort and its active principles in depression and anxiety Basel. J Med Chem 2006;49:5026–7.
Kristina Leuner, Victor Kazanski, Margarethe Müller, Kirill Essin, Bettina Henke, Maik Gollasch, et al. Müller hyperforin-a key constituent of St. John’s wort specifically activates TRPC6 channels. FASEB J 2007;21:4101-11.
Schempp C, Müller K, Winghofer B, Schöpf E, Simon J. Johanniskraut (Hypericum perforatum L.). Eine Pflanze mit Relevanz für die Dermatologie. Der Hautarzt 2002;53:316–21.
De Freitas Silva DM, Ferraz VP, Ribeiro AM. Improved high-performance liquid chromatographic method for GABA and glutamate determination in regions of the rodent brain. J Neurosci Methods 2009;177:289–93.
Umek A, Kreft S, Kartnig T, Heydel B. Quantitative phytochemical analyses of six Hypericum species growing in slovenia. Planta Med 1999;65:388–90.
Exarchou V, Nenadis N, Tsimidou M, Gerothanassis IP, Troganis A, Boskou D. Antioxidant activities and phenolic composition of extracts from greek oregano, greek sage, and summer savory. J Agric Food Chem 2002;50:5294–9.
Conforti F, Statti GA, Tundis R, Menichini F, Houghton P. Antioxidant activity of methanolic extract of Hypericum triquetrifolium turra aerial part. Fitoterapia 2002;73:479–83.
Tripathi YB, Pandey E, Dubey GP. Antioxidant property of Hypericum perforatum (L.) of Indian origin and its comparison with established Medhya rasayanas of Ayurvedic medicine. Curr Sci 1999;76:27–9.
Ilkay Erdogan Orhan, Berrin Ozcelik, Belma Konuklugil, Annika Putz, Ulku Gokcen Kaban, Peter Proksch. Bioactivity screening of the selected turkish marine sponges and three compounds from agelas oroides. Rec Nat Prod 2012;6:356-67.
Kızıl G, Kızıl M, Yavuz M, Emen S, Hakimoğlu S. Antioxidant activities of ethanol extracts of Hypericumtriquetrifolium and Hypericumscabroides. Pharm Biol 2008;46:231-42.
Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid-rich extract of Hypericumperforatum L. in vitro. J Agric Food Chem 2004;52:5032-9.
Rice-Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2:152-9.
Couladis M, Badisa RB, Baziou P, Chaudhuri SK, Pilarinou E, Verykokidou. Antioxidant and cytotoxic activities of Hypericum sp. On brine shrimps and human cancer cell lines. Phytother Res 2002;16:719-22.
Silva BA, Ferreres F, Malva JO, Dias ACP. Phytochemical and antioxidant characterization of Hypericumperforatum alcoholic extracts. Food Chem 2005;90:157-67.
Kasper S, Caraci F, Forti B, Drago F, Aguglia E. Efficacy and tolerability of Hypericum extract for the treatment of mild to moderate depression. Eur Neuropsychopharmacol 2010; 20:747–65.
Ozturk Y, Aydin S, Beis B, Baser KHC, Berberoglu H. Effects of H. perforatum and H. calycinum extracts on central nervous system in mice. Phytomedicine 1996;3:139-46.
Erasmus RT, Savory J, Wills MR. Aluminum neurotoxicity in experimental animals. Ther Drug Monit 1993;15:588-92.
Strong MJ, Garruto RM, Joshi JG. Can the mechanisms of aluminum neurotoxicity be integrated into a unified scheme? J Toxicol Environ Health 1996;48:599-613.
Ouafa Rebai, Nour Eddine Djebli. Chronic exposure to aluminum chloride in mice: exploratory behaviors and spatial learning. Adv Biol Res 2008;2:26-33
Pellow S. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985;14:149-67.
Rodgers RJ. Ethopharmacological analysis of the effects of putative 'anxiogenic' agents in the mouse elevated plus-maze. Pharmacol Biochem Behav 1995;52:805-13.
Rodgers RJ, NJ Johnson. Factor analysis of spatiotemporal and ethological measures in the murine elevated plus-maze test of anxiety. Pharmacol Biochem Behav 1995;52:297-303.
Moraira EG, Nascimento N, Rogero JR. Gaba-ergicbenzodiazepine system is involved in the crotoxin-induced anxiogenic effect. Pharmacol Biochem Behav 2000;65:7-13.
Rodriguez Echandia EL, Broitman ST, Foscolo MR. Effect of the chronic ingestion of chlorimipramine and desipramine on the hole board response to acute stresses in male rats. Pharmacol Biochem Behav 1987;26:207-10.
Takeda H, Tsuji M, Matsumiya T. Changes in head-dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. Eur J Pharmacol 1998;350:21-9.
Xiuyan Wei, Jingyu Yang, Chunfu Wu. Anxiolytic effect of baicalin in mice. Asian J Tradit Med 2006;1:3-4.
Jorge A. Quillfeldt; behavioral methods to study learning and memory in rats. Library of congress. Springer link; 2006.
Balazs L, Leon M. Evidence of an oxidative challenge in the Alzheimer brain. Neurochem Res 1994;19:1131-7.
Marcus DL, Thomas C, Rodriguez C, Simberkoff K, Tsai JS, Strafaci JA. Increased peroxidation and reduced antioxidant enzyme activity in Alzheimer’s disease. Exp Neurol 1998;150:40–4.
Saija A, Scalese M, Lanza M, Marzullo D, Bonina F, Castelli F. Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radical Biol Med 1995;19:481–6.
Bhakta Prasad Gaire, Hocheol Kim. Neuroprotective effects of fructus chebulae extracts on experimental models of cerebral ischemia. J Tradit Chin Med 2014;34:69-75.