• Asma Mosbah Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Setif 1, Setif 19000 Algeria, Department of Applied biology, Faculty of Natural and Life sciences, Mentouri brothers University, Constantine 25000 Algeria
  • Widad Sobhi Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Setif 1, Setif 19000 Algeria
  • Mustapha Benboubetra Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Setif 1, Setif 19000 Algeria


Nigella sativa, Total oil, Neutral lipid fraction, Phenolic content, Flavonoid content, Antioxidant activity, Anti-hemolytic activity


Objective: Nigella sativa (NS) oil is usually considered as oxidatively stable, the aim of this study is to evaluate its antioxidant capacity in vitro through four mechanisms.

Methods: NS seed total oil (TO) was extracted with n-hexane and this oil was further fractionated into neutral lipid fraction (NLF) and polar lipid fraction. Total oil and its neutral lipid fraction were investigated for the antioxidant activity using the following spectrophotometric assays; DPPH and ABTS radicals scavenging tests, reducing power test, ß-carotene bleaching test and anti-hemolytic test. All tests were realized by spectrophotometric methods.

Results: Quantitative determination of phenols and flavonoids in TO and NLF showed that TO has the highest phenolic and flavonoid concentrations with 26,44±0,21 µg GAE/g extract and 1.34±0.21 µg QAE/g extract respectively. All antioxidant assays showed that TO has the strongest antioxidant capacity with; IC50 83.023±4.58 µg/ml in DPPH scavenging assay, IC50 80.09±10.51 µg/ml in ABTS scavenging assay, EC50 85.93±20.01 µg/ml in reducing power assay and a percentage of inhibition 60.62 % in ß-carotene bleaching assay, equally, TO has the strongest anti-hemolytic activity with HT50 of 76.96±1.22 µg/ml.

Conclusion: The results obtained in this investigation showed that Nigella sativa has an important antioxidant capacity, which makes it an attractive source of new therapeutic components.


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Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans 2007;35:1147-50.

Beckman KB, Ames BN. Endogenous oxidative damage of mt DNA. Mutat Res 1999;424:51-8.

Wei YH, Lu CY, Wei CY, Ma YS, Lee HC. Oxidative stress in human aging and mitochondrial disease-consequences of defective mitochondrial respiration and impaired antioxidant enzyme system. Chin J Physiol 2001;44:1-11.

Mansour MA, Ginawi OT, El-Hadiyah T, El-Khatib AS, Al-Shabanah OA, Al-Sawaf HA. Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride-induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone. Res Commun Mol Pathol Pharmacol 2001;110:239-51.

Przybylski R, Lee YC, Eskin NAM. Antioxidant and radical scavenging activities of buckwheat seed components. J Am Oil Chem Soc 1998;75:1595-600.

Koski A, Psomiadou E, Tsimidou M, Hopia A, Kefalas P, Wa ¨ha ¨la K, et al. Oxidative stability and minor constituents of virgin olive oil and cold-pressed rape seed oil. Eur Food Res Technol 2002;214:294-8.

Lee JC, Kim HR, Kim J, Jan YS. Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. Saboten J Agric Food Chem 2002;50:6490-6.

Yanishlieva NV, Marinova EM. Stabilisation of edible oils with natural antioxidants. Eur J Lipid Sci Technol 2001;103:752-67.

Ramadan MF, Lothar WK, Jorg-TM. Radical scavenging activity of black cumin (Nigella sativa L.), Coriander (Coriandrum sativum L.), and Niger (Guizotia abyssinica Cass.) crude seed oils and oil fractions. J Agric Food Chem 2003;51:6961−9.

Ramadan MF, Mörsel JT. Characterization of phospholipid composition of black cumin (Nigella sativa L.) seed oil. Nahrung 2002;46:240-4.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and anti-oxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999;299:152-78.

Bahorun T, Gressier B, Trotin F, Brunete C, Dine T, Vasseur J, et al. Oxigen species scavenging activity of phenolic extract from howthorn fresh plant organs and pharmaceutical preparation. Arzneim-Forsch 1996;46:1086-94.

Re R, Pellegrinni N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radic Biol Med 1998;26:1231–7.

Rubalya VS, Neelamegam P. Antioxidant potential in vegetable oil. Res J Chem Environ 2012;16:87-94.

Schlesier K, Harwat M, Bohm V, Bitsch R. Assessment of antioxidant activity by using different in vitro methods. Free Radic Res 2002;36:177-87.

Barros L, Ferreira MJ, Queiros B, Ferreira ICFR, Baptista P. Total phenols, ascorbic acid, b-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 2007;103:413-9.

Kartal N, Sokmen M, Tepe B, Daferera D, Polissiou M, Sokmen A. Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food Chem 2007;100:584-9.

Albertini MC, Chibelli L, Ricciotti R, Fumelli C, Canestrari F, Galli F, et al. Morphological alterations and increased resistance to hemolysis in t-butyl hydroperoxide incubated RBC from elderly subjects. Arch Gerontol Geriatr 1996;22:423-8.

Dwight JFJ, Hendry BM. The effects of tert-butyl hydroperoxide on human erythrocyte membrane ion transport and the protective actions of antioxidants. Clin Chim Acta 1996;249:167-81.

Sullivan SG, Stern A. Membrane protein changes induced by tert-butyl hydroperoxide in red blood cells. Biochim Biophys Acta 1984;774:215-20.

Meziti A, Meziti H, Boudiaf K, Benboubetra M, Bourich H. Polyphenolic profile and antioxidant activities of Nigella sativa seed extracts in vitro and in vivo. World Acad Sci Eng Technol 2012;64:24-32.

Amarowicz R, Naczk M, Shahidi F. Antioxidant activity of crude tannins of canola and rapeseed hulls. J Am Oil Chem Soc 2000;77:957-61.

Tovar MJ, Motilva J, Romero MP. Changes in the phenolic composition of virgin olive oil from young trees (Olea europaea L. cv. Arbequina) grown under linear irrigation strategies. J Agric Food Chem 2001;49:5502-8.

Hudson BJF, Lewis JI. Polyhydroxy flavonoid antioxidants for edible oils: phospholipids as synergists. Food Chem 1983;10:111-20.

Hildebrand DH, Terao J, Kito M. Phospholipids plus tocopherols increase soybean oil stability. J Am Oil Chem Soc 1984;61:552-5.

Frankel EN, Huang SW, Kanner J, German JB. Interfacial phenomena in the evaluation of antioxidants: bulk oils vs. emulsions. J Agric Food Chem 1994;42:1054-9.

Arbos KA, Claro LM, Borges L, Santos CAM, Weffort-Santos AM. Human erythrocytes as a system for evaluating the antioxidant capacity of vegetable extracts. Nutr Res 2008;28:457–63.

Çimen MYB. Free radical metabolism in human erythrocytes. Clin Chim Acta 2008;39:1-11.

Stocker P, Lesgards JF, Vidal N, Chalier F, Prost M. ESR study of a biological assay on whole blood: antioxidant efficiency of various vitamins. Biochim Biophys Acta 2003;1621:1-8.

Djeridane A, Yousfi M, Nadjemi B, Vidal N, Lesgards JF, Stocker P. Screening of some Algerian medicinal plants for the phenolic compounds and their antioxidant activity. Eur Food Res Technol 2007;224:801-9.



How to Cite

Mosbah, A., W. Sobhi, and M. Benboubetra. “ANTIOXIDANT AND ANTI-HEMOLITIC ACTIVITIES OF ALGERIAN NIGELLA SATIVA TOTAL OIL AND ITS NEUTRAL LIPID FRACTION”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 50-54,



Original Article(s)