ANTIOXIDANT AND ANTI-HEMOLITIC ACTIVITIES OF ALGERIAN NIGELLA SATIVA TOTAL OIL AND ITS NEUTRAL LIPID FRACTION
Keywords:
Nigella sativa, Total oil, Neutral lipid fraction, Phenolic content, Flavonoid content, Antioxidant activity, Anti-hemolytic activityAbstract
Objective: Nigella sativa (NS) oil is usually considered as oxidatively stable, the aim of this study is to evaluate its antioxidant capacity in vitro through four mechanisms.
Methods: NS seed total oil (TO) was extracted with n-hexane and this oil was further fractionated into neutral lipid fraction (NLF) and polar lipid fraction. Total oil and its neutral lipid fraction were investigated for the antioxidant activity using the following spectrophotometric assays; DPPH and ABTS radicals scavenging tests, reducing power test, ß-carotene bleaching test and anti-hemolytic test. All tests were realized by spectrophotometric methods.
Results: Quantitative determination of phenols and flavonoids in TO and NLF showed that TO has the highest phenolic and flavonoid concentrations with 26,44±0,21 µg GAE/g extract and 1.34±0.21 µg QAE/g extract respectively. All antioxidant assays showed that TO has the strongest antioxidant capacity with; IC50 83.023±4.58 µg/ml in DPPH scavenging assay, IC50 80.09±10.51 µg/ml in ABTS scavenging assay, EC50 85.93±20.01 µg/ml in reducing power assay and a percentage of inhibition 60.62 % in ß-carotene bleaching assay, equally, TO has the strongest anti-hemolytic activity with HT50 of 76.96±1.22 µg/ml.
Conclusion: The results obtained in this investigation showed that Nigella sativa has an important antioxidant capacity, which makes it an attractive source of new therapeutic components.
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