INDIRECT SPECTROPHOTOMETRIC ESTIMATION OF DRUGS USING CERIUM (IV) AND RHODAMINE-B AS ANALYTICAL REAGENT
Keywords:Spectrophotometry, Drugs, Cerium, Rhodamine-B, Quantification and Validation
Objective: The object of this research was to develop simple, sensitive and selective methods for the quantitative determination of five drugs, viz., (RAM) Ramipril, (VOR) Voriconazole, (SDC) Sildenafil Citrate, (ITM) Imatinib mesylate and (CFH) Ciprofloxacin hydrochloride.
Methods: The method for each drug depends upon oxidation of drugs by Cerium [Ce (IV)] (Excess) and estimating the amount of unreacted Ce (IV) by Rhodamine-B dye at 557 nm. These methods have been applied to the determination of drugs in their pure form as well as in tablet formulations.
Results: Beer's law is obeyed in the concentration of 16-112, 15-105, 12-84, 8-56 and 2-14 Î¼g ml-1 for (RAM) Ramipril, (VOR) Voriconazole, (SDC) Sildenafil Citrate, (ITM) Imatinib mesylate and (CFH) Ciprofloxacin hydrochloride respectively. The results of analyses were validated statistically. Statistical comparison of the results with the reference method shows excellent agreement and indicates no significant difference in accuracy and precision. The effect of excipients has also been studied and found to have no effect. These methods have been validated in terms of guidelines of ICH.
Conclusion: The proposed methods have good selectivity and a correct sensibility; they rely on the use of simple and cheap chemicals, but provide similar sensitivity to that obtained with the HPLC; sophisticated and more expensive technique.
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