HUMAN SECRETORY PHOSPHOLIPASE A2 (sPLA2) INHIBITION BY AQUEOUS EXTRACT OF MACROTYLOMA UNIFLORUM (SEED) AS AN ANTI-INFLAMMATORY ACTIVITY

Giresha A. S.; Narayanappa M; Vikram Joshi; Vishwanath B. S.; Dharmappa K. K.

Authors

Giresha A. S. Department of Studies in Biochemistry, Mangalore University, P. G Centre, Chikka Aluvara
Narayanappa M Department of Biotechnology, Government Science College, Hassan,
Vikram Joshi Department of Studies in Biochemistry, University of Mysore, Mysore
Vishwanath B. S. Mysore university
Dharmappa K. K. Department of Studies in Biochemistry, Mangalore University, P. G Centre, Chikka Aluvara

Keywords:

HSF, Human Synovial Fluid, HPF, Human Pleural Fluid, Anti-inflammatory, sPLA2, secretory Phospholipase A2, VRV-PLA2, Vipera russelli PLA2, NN-PLA2, Naja naja PLA2, COX-12, Cyclo-oxygenase-12, LOX, Lipoxigenase, Lipid peroxidation

Abstract

Objective: Macrotyloma uniflorum (Horse gram) is an important legume widely consumed in the tropical south Asian countries including India. The present investigation is the elucidation of anti-inflammatory activity of M. uniflorum as it has several medicinal properties. The M. uniflorum was evaluated for inhibition of human secretory phospholipase A₂(sPLA₂) as a function of anti-inflammatory activity.

Methods: The total phenols, antioxidant (DPPH scavenging), Anti-lipid peroxidation, PLA₂ inhibition and lipoxygenase (5 & 15-LOX) inhibition activity of aqueous extracts of M. uniflorum coat and pulp were assayed by in vitro method. The aqueous extract of M. uniflorum seed coat was subjected to inhibit PLA₂enzymes from human inflammatory fluids (Human Synovial Fluid and Human Pleural Fluid) and snake venoms (Naja naja and Vipera russllii) using [14]C labeled E. coli by in vivo method. A further effect of substrate and calcium concentration on inhibition of VRV-PLA₂ in presence and absence of M. uniflorum coat extract were assayed.

Results: Aqueous coat extract of M. uniflorum shows higher phenolics and biological activity and inhibited all sPLA₂enzymes in concentration dependent manner. The IC₅₀values are found to be in the range of 11.42-20.88Î¼g and IC₅₀values for 5-LOX and 15-LOX is 25.92Î¼g and 32.47Î¼g respectively. The extract effectively neutralized indirect hemolytic activity and showed similar potency in neutralizing the in vivo sPLA₂ induced mouse paw edema.

Conclusion: These findings suggest that, the active compound/s in extracts of M. uniflorum individually or synergistically responsible for observed sPLA₂ inhibition.

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Author Biographies

Giresha A. S., Department of Studies in Biochemistry, Mangalore University, P. G Centre, Chikka Aluvara

Mangalore university is one of the main university in india, which is in top 50 and A grade with NACC

Narayanappa M, Department of Biotechnology, Government Science College, Hassan,

Biotechnology

Vikram Joshi, Department of Studies in Biochemistry, University of Mysore, Mysore

deportment of studies in biochemistry

Vishwanath B. S., Mysore university

Biochemistry

Dharmappa K. K., Department of Studies in Biochemistry, Mangalore University, P. G Centre, Chikka Aluvara

biochemistry

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