• Benyamina Amel University of Oran
  • Kharoubi Omar University of Oran 1 (Ahmed Benbella), Faculty of Natural and Life Sciences, Department of Biology, Oran Algeria
  • Fodil Faiza University of Oran
  • Slimani Miloud University of Oran
  • Aoues Abdelkader University of Oran


Lead, Whole brain, Glutamate enzymes, Lipid peroxidation, Behavior changes


Objective: Lead poisoning induced severe behavioral abnormalities and impaired cognitive functions in experimental animals. The aim of the present study is to investigate the detrimental effects of lead exposure on the behavior of rats and its association with altered neurochemistry.

Methods: Twenty-four young male Wistar rats were divided into 4 groups: G1: a control group receiving drinking water. G2: intoxicated group (Pb) exposed to lead acetate (1000 ppm in drinking water). G3: receives Wormwood aqueous (A. Ab) extract at a dose of 300 mg/l in drinking water. G4: rats are receiving Pb+A. Ab mixture for 4 additional weeks after intoxication for 8 w. In the present study, locomotors activity in rats was assessed by open field test (OFT) while anxiety and depressive behavior were monitored by elevated plus maze (EPM) and the forced swim test (FST), the evaluation of glutamate metabolizing enzymes in whole brain and lipid peroxidation was carried out in all groups.

Results: our results showed that lead acetate intoxication increased the level of lipid peroxidation in brain, decreased brain glutamate oxaloacetate transaminase activities and increased glutamate pyruvate transaminase. Also, lead (pb) exposure resulted in increased anxiety and fear-related behavior in both elevated plus maze and light dark box tests, showed hyperactivity in open field test presented by increased horizontal locomotion. However, A. Ab extract reduced the TBARS level by preventing oxidative stress induced by lead and increased glutamate pyruvate transaminase activity.

Conclusion: The wormwood extract administration reduced anxiety, fear and locomotion and improved learning ability and memories. Therefore, these results indicated that wormwood is ameliorating the deleterious effects of lead and it appeared to be a protective agent against lead-induced toxicity.



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Van Wijngaarden E, Campbell JR, Cory-Slechta DA. Bone Pb levels are associated with measures of memory impairment in older adults. Neurotoxicology 2009;30:572-80.

Friend TH. Symposium: response of animals to stress. J Dairy Sci 1991;74:292-303

Yang Y, Ma Y, Ni L, Zhao S, Li L, Zhang J, et al. Pb exposure through gestation-only caused long-term learning/memory deficits in young adult offspring. Exp Neurol 2003;184:489-95.

De Souza Lisboa SF, Gonçalves G, Komatsu F, Queiroz CA, Almeida AA, Moreira EG. Developmental Pb exposure induces depressive-like behavior in female rats. Drug Chem Toxicol 2005;28:67-77.

Kharoubi O, Slimani M, Aoues A. Neuroprotective effect of wormwood against lead exposure. J Emergencies Trauma Shock 2011;4:82–8.

Bressler JP, Goldstein GW. Mechanisms of lead neurotoxicity. Biochem Pharmacol 1991;41:479-84.

Hölscher C. Stress impairs performance in spatial water maze tasks. Behav Brain Res 1999;100:225-35.

Siddique MS, Eddeb F, Mantle D, Mendelow AD. Extracts of ginkgo biloba and panax ginseng protect brain proteins from free radical induced oxidative damage in vitro. Acta Neurochir 2000;76:87–90.

Engelhart MJ, Geerlings MI, Ruitenberg A, van Swieten JC, Hofman A, Witteman JC, et al. Dietary intake of antioxidants and risk of Alzheimer disease. JAMA 2002;287:3223–9.

Tapiero H, Tew KD, Ba GN, Mathé G. Polyphenols: do they play a role in the prevention of human pathologies? Biomed Pharmacother 2002;56:200–7.

Ponnusamy K, Mohan M, Nagaraja HS. Protective antioxidant effect of Centella asiatica bioflavonoids on lead acetate induced neurotoxicity. Med J Malaysia 2008;63 (Suppl A):102-5.

Wake G, Court J, Pickering A, Lewis R, Wilkins R, Perry E. CNS acetylcholine receptor activity in European medicinal plants traditionally used to improve failing memory. J Ethnopharmacol 2000;69:105-14.

Guarrera PM. Traditional phytotherapy in central Italy (Marche, Abruzzo, and Latium). Fitoterapia 2005;76:1–25.

Harendra SP, Gang L, Ming QWA. A new dawn for the use of traditional Chinese medicine in cancer therapy. Molecular Cancer 2009;8:21.

ÄŒanadanović-Brunet JM, Äilas SM, Ćetković G, Tumbas VT. Free-radical scavenging activity of wormwood (Artemisia absinthium L.) extracts. J Sci Food Agric 2005;85:265–72.

Streecher HJ. Transaminases. In: Handbook of Neurochemistry (Edited by: A Lajtha) Plenum Press: New York; 1970. p. 173-92.

Gilani AH, Janbaz KH. Preventive and curative effects of Artemisia absinthium on acetaminophen and CCl4-induced hepatotoxicity. Gen Pharmacol 1995;26:309-15.

Auclair A, Drouin C, Cotecchia S, Glowinski J, Tassin JP. 5-HT2A and alpha1b-adrenergic receptors entirely mediate dopamine release, locomotor response and behavioral sensitization to opiates and psychostimulants. Eur J Neurosci 2004;20:3073–84.

Bergmeyer HU, Bernt E. Glutamate oxaloacetate transaminase, Glutamate pyruvate transaminase. In: Methods of Enzymatic Analysis. Edited by Bergmeyer HU. Academic Press: New York; 1963. p. 837-52.

Niehius WG, Samuelsson D. Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation. Eur J Biochem 1968;6:126-30.

Millan M. The neurobiology and control of anxious states. Prog Neurobiol 2003;70:83-244.

Cauli O, Morelli M. Subchronic caffeine administration sensitizes rats to the motor-activating effects of dopamine D1 and D2 receptor agonists. Psychopharmacology 2002;162:246-54.

Trullas R, Skolnick P. Differences in fear-motivated behaviors among inbred mouse strains. Psychopharmacol 1993;111:323-31.

Walf A, Frye C. The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat Protoc 2007;2:322-8.

Costall B, Domeney A, Gerrard MP, Kelly AM, Naylor ER. Zacopride: anxiolytic profile in rodent and primate models of anxiety. Afr J Pharm Pharmacol 1988;40:302-5.

Albuquerque E. Receptors in lead-induced cognitive deficit. Crip Data Base National Institutes Health; 1995.

Streecher HJ, Transaminases. In: Handbook of Neurochemistry. Edited by: A Lajtha. Plenum Press: New York; 1970. p. 173-92.

Schulz JB, Mathews RT, Henshaw DR, Beal MF. Neuroprotective strategies for treatment of lesions produced by mitochondrial toxins: implication for neurodegenerative diseases. Neuroscience 1996,71:1043-8.

Prasunpriya N, Ajay KC. The response of regional brain glutamate transaminases of rat to aluminum in protein malnutrition. BMC Neuroscience 2002;3:12.

Matthews CC, Zielke HR, Wollack JB, Fishman PS. Enzy-matic degradation protects neurons from glutamate excitotoxicity. J Neurochem 2000;75:1045-52.

Flora SJ, Saxena G, Mehta A. Reversal of lead-induced neuronal apoptosis by chelation treatment in rats: role of ROS and intracellular Ca2+. J Pharmacol Exp Ther 2007;322:108–16.

Guilarte, Tomás R, McGlothan Jennifer L. Selective decrease in NR1 subunit splice variant mRNA in the hippocampus of Pb2+-exposed rats: implications for synaptic targeting and cell surface expression of NMDAR complexes. Mol Brain Res 2003;113:37–43.

Bennet C, Bettaiya R, Rajanna S, Baker L, Yallapragada PR, Brice JJ, et al. Region-specific increase in the antioxidant enzymes and lipid peroxidation products in the brain of rats exposed to lead. Free Radic Res 2007;41:267–73.

Fischbein A. Occupational and environmental lead exposure. In: Environmental and occupational medicine. Rom WN.(ed). 2nd ed. Boston Little. Brown; 1999. p. 735-58.

Hassan AA, Jassim HM. Effect of treating lactating rats with lead acetate and its interaction with vitamin E or C on neurobehavior, development and some biochemical parameters in their pups. Iraqi J Veterinary Sci 2010;24:45-52.

Satija NK, Vij AG. Preventive action of zinc against lead toxicity. Indian J Physiol Pharmacol 1995;39:377–82.

Ahamed M, Siddiqui MK. Low level lead exposure and oxidative stress: current opinions. Clin Chim Acta 2007;383:57–64.

Maged MY. Prophylactic efficacy of crushed Garlic lobes, Black seed Olive oils on cholinesterase activity in central nervous system parts and serum of lead intoxication rabbits. Turk J Biol 2005;29:173-80.

Seddik L, Bah TM, Aoues M, Benderdour M, Slimani M. Dried leaf extract protects against lead-induced neurotoxicity in wistar rats. Eur J Sci Res 2010;42:139-51.

Trombini TV, Pedroso CG, Ponce D, Almeida AA, Godinho AF. Developmental lead exposure in rats: Is a behavioral sequel extended at F2 generation? Pharmacol Biochem Behavior 2001;68:743-51.

Benammi H, Omar E, Abderrahmane R, Halima G. A blunted anxiolytic-like effect of curcumin against acute lead induced anxiety in rat: Involvement of serotonin. Acta Histochem 2014;116:920–5.

Bouayed J, Rammal H, Younos C, Soulimani R. Positive correlation between peripheral blood granulocyte oxidative status and level of anxiety in mice. Eur J Pharmacol 2007;564:146–9.



How to Cite

Amel, B., K. Omar, F. Faiza, S. Miloud, and A. Abdelkader. “BEHAVIOR AND GLUTAMATE TRANSAMINASE CHANGES IN RAT EXPOSED TO LEAD AND TREATED BY WORMWOOD EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 2, Feb. 2016, pp. 208-13,



Original Article(s)