• Aiche Iratni Ghenima Université Mouloud MAMMERI de Tizi-Ouzou
  • Moualek Idir Université Mouloud MAMMERI de Tizi-Ouzou
  • Mestar Guechaoui Nadjet Université Mouloud MAMMERI de Tizi-Ouzou
  • Mezaache Aichour Samia Université Ferhat Abbas Sétif 1
  • Zerroug Mohamed Mihoub Université Ferhat Abbas Sétif 1
  • Houali Karim Laboratoire de Recherche Biochimie Analytique and Biotechnologie (LABAB). Faculté des Sciences Biologiques et des Sciences Agronomiques. Université Mouloud MAMMERI de Tizi-Ouzou. Algérie


Pistacia lentiscus, Antioxidant, Haemolysis


Objective: The aim of this study was to study the antioxidant potential and the antibacterial activity of leaves aqueous extract of Pistacia Lentiscus as well as the protective effect of this extract against the haemolysis in hypotonic condition, in oxidative stress and in the existence of saponin injury.

Methods: We studied the antioxidant capacity through the DPPH assay, H2O2 scavenging activity, Ferric-reducing power (FRAP) assay, total antioxidant assay and the antibacterial activity using the disc diffusion method. We also investigated the haemolytic activity with the spectrophotometric method.

Results: The result showed that the aqueous extract had a good antioxidant capacity, which was calculated as IC50. IC50 of the aqueous extract was found to be 9.89±0.7µg/ml for DPPH scavenging, 200±18.02µg/ml for H2O2 scavenging assay, 54.06±12.66µg/ml for Ferric-reducing power (FRAP) and 500±22.3 µg/ml for total antioxidant capacity. The aqueous extract also inhibited the growth of the tested bacterial strains with a maximum inhibition zone of 30.33±5.5 mm observed on Pseudomonas aeruginosa for wood-seed and a moderate activity against all other strain. The haemolytic analysis showed that the aqueous extract is not toxic for the human erythrocytes and protects them against the oxidative and osmotic stress and also against saponin injury.

Conclusion: The results of our study suggest that the aqueous extract of leaves of Pistacia lentiscus possess potent anti-haemolytic activity, are a good source of natural antioxidant.



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Shabana A, El-Menyar A, Asim M, Al-Azzeh H, Al Thani H. Cardiovascular benefits of black cumin (Nigella sativa). Cardiovasc Toxicol 2013;13:9-21.

Fantini M, Benvenuto M, Masuelli L, Frajese GV, Tresoldi Modesti A, Bei R. In vitro and in vivo antitumoral effects of combinations of polyphenols, or polyphenols and anticancer drugs: Perspectives on Cancer treatment. Int J Mol Sci 2015;16:9236-82.

Rehman H, Krishnasamy Y, Haque K, Thurman RG, Lemasters JJ, Rick G, et al. Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats. PLoS One 2013;8:e65029.

Margaris NS. Adaptative stratgis inplants dominating mediterrane-type ecosystem. In: Di Castri F, Goodal DW, Spech RL. Ecosystem of the world 11. Meditrranean-type Shrublands. New York: Elsevier Science; 1993. p. 309-15.

Bacchetta G, Cappai G, Carucci A, Tamburini E. Use of native plants for the remediation of abandoned mine sites in Mediterranean semiarid environments. Bull Environ Contam Toxicol 2015;94:326-33.

Djerrou Z. Anti-hypercholesterolemic effect of Pistacia Ientiscus fatty oil in egg yolk-fed rabbits: a comparative study with simvastatin. Chin J Nat Med 2014;12:561-6.

Giaginis C, Theocharis S. Current evidence on the anticancer potential of Chios mastic gum. Nutr Cancer 2011;63:1174-84.

Azatzeh H, Teiahfert F, Abbasfi Markovics A, Muktada H, Ungar ED, Landau SY. Polyphenols from Pistacia Ientiscus and Phillyrea latifolia impair the exsheathment of gastro-intestinal nematode larvae. Vet Parasitol 2013;191:44-50.

Mezni F, Aouadhi C, Khouja ML, Khartii A, Maaroufi A. In vitro antimicrobial activity of Pistacia Ientiscus L. edible oil and phenolic extract. Nat Prod Res 2015;29:565-70.

Joshua DL, Shengmin S, Chung SY. Possible contreversy over dietary polyphenols: benefits vs ricks. Chem Res Toxicol 2007;20:583-5.

Cao G, Sofic E, Prtor RL. Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radical Biol Med 1997;22:749-60.

Sadegh F, Ebrahim Z, Zeïnab A, Roghayeh P, Ali MA, Amrollah M, et al. Total Phenolic and Flavonoid contents of aqueous extract of stinging nettle an in vitro antiproliferative effect on Hela and BT-474 cell lines. Int J Mol Cell Med 2014;3:102-7.

Jayaprakasha GK, Jaganmohan RL, Sakariah KK. Antioxidant activities of flavidin in different in vitro model systems. Bioorg Med Chem 2004;12:5141-6.

Fejes S, Blà zovics A, Luqasi A, Lemberkovics E, Pétri G, Kéry A. In vitro antioxidant activity of Anthriscus cerefolium L. (Hoffm.) extracts. J Ethnopharmacol 2000;69:259-65.

Pingping W, Guangzhi M, Nianghui L, Qian D, Yanyan Y, Ruqiang H. Investigation of in vitro and in vivo antioxidant activities of flavonoids rich extract from the berries of Rhodomyrtus tomentosa (Ait.) Hassk. Food Chem 2015;173:194-202.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.

Koga T, Moro K, Terao J. Protective effect of a vitamin E analog, phosphatidylchromanol, against oxidative hemolysis of human erythrocytes. Lipids 1998;33:589-95.

Duchnowicz P, Milena BM, Podsedek A, Koter-Michalak M. Effect of polyphenols extracts from Brassica vegetables on erythrocyte membranes (in vitro study). Environ Toxicol Pharmacol 2012;34:783-90.

de Freitas MV, Netto Rde C, da Costa Huss JC, de Souza TM, Costa JO, Firmino CB, et al. Influence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytes. Toxicol In vitro 2008;22:219-24.

Battistelli M, De Sanctis R, De Bellis R, Cucchiarini L, Dacha M, Gobbi P. Rhodiola Rosea as antioxidant in red blood cells: ultrastructural and haemolytic behaviour. Eur J Histochem 2005;49:243-54.

Bougandoura N, Bendjimerad N. Evaluation de l'activité antioxydante des extraits aqueux et méthanolique de Satureja calamintha ssp. Nepla (L) Briq. Nature Technol 2013;9:14-9.

Gawron-Gzella A, Duked-Makuch M, Matlawski I. DPPH radical scavenging activity and polyphenolic compound conten in different leaf extract from selected blackberry species. Acta Biol Cracov Ser Bot 2012;54:32-8.

Bors M, Siciska P, Michalowicz J, Wieteska P, Bukowska P. Evaluation of the effect of tomentosa extracts on the size and shape of human erythrocytes (in vitro). Environ Toxicol Pharmacol 2012;23:127-34.

Nasem SA, Farmah M, Najmi MH. Pharmacological basis for use of Pistacia integerrima leaves. J Ethomopharmacol 2008;117:478-82.

Saikat S, Biplab D, Devanna N, Raja C. Total phenolic, total flavonoid content, and antioxidant capacity of leaves of Meyma spinosa Roxb., an Indian medicinal plant. Chin J Nat Med 2013;11:149-57.

Nabavi SF, Ibrahimzadeh MA, Nabavi SM. Antihemolytic and antioxydant activities of Biebersteinia multifida. Eur Rev Med Pharmacol Sci 2010;14:823-30.

Chamira DF, Preethi S. Total phenolic, flavonid contents, in vitro antioxydant activities and hepatoprotective effect of aqueous leaf extract of Atalantia ceylanica. BMC Complementary Altern Med 2014;14:395.

Spag SG, Light ME, Staden JV. Biological activities and distribution of plant saponin. J Ethnolopharmacol 2004;94:219-43.

Suwalsky M, Orellana P, Avello M, Villena F. Protective effect of Ugni molinae Turcz against oxidative domage of human erythrocytes. Food Chem Toxicol 2007;45:130-5.

He J, Lin J, Li J, Zhang JH, Sun XM, Zeng CM. Dual effectsof ginkgo biloba leaf extract on human red blood cells. Basic Clin Pharmacol Toxicol 2008;104:139-44.

Wloch A, Kapusta I, Bielecki K, Oszmianski J, Kleszczyńska H. Activity of Hawthorn leaf and bark extract in relation to biological membrane. J Membr Biol 2013;246;545-56.

Nabavi SM, Nabavi SF, Setzer W, Alinezhad H, Zare M, Naqinezhad A. Interaction of different extracts of Primula heterochroma Stapf. With red blood cell membrane lipids and proteines: antioxydant and antihemolytic effects. J Diet Suppl 2012;9:285-92.

Juscova M, Snirc V, Krisonova L, Stefek M. Effect of carboxymethylated pyridoindoles on free radical induced heamolysis of rat erythrocytes in vitro. Acta Biochim Pol 2010;57:153-6.

Iank L, Ragausa S, Rapisarda A, Franco S, Nicolsi VM. In vitro antimicrobial activity of Pistacia lentiscus L. extract: preliminary report. J Chemother 1996;8:207-9.

Hamad HH, Ibrahim HH, Mariam HG, Mojahidul I. Comparative phytochemical and antimicrobial investigation of some plants growing in Al Jabal Al-Akhdar. J J Nat Prod Plant Resour 2011;1:15-23.

Shaikh I, Mohd T, Syed JA, Shahid SC, Mirza GB. Pistacia lentiscus Linn.: Gum with immense medicinal potential. Spatula DD 2013;3:69-73.



How to Cite

Ghenima, A. I., M. Idir, M. G. Nadjet, M. A. Samia, Z. M. Mihoub, and H. Karim. “IN VITRO EVALUATION OF BIOLOGICAL ACTIVITIES OF PISTACIA LENTISCUS AQUEOUS EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 133-9,



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