ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF SIX ALGERIAN MEDICINAL PLANTS
Keywords:
Polyphenols, Antioxidant, Antibacterial, DPPH, FRAP, NilAbstract
Objective: This work had two objectives: the first, to evaluate the total phenolic or flavonoid contents of six Algerian medicinal plants and second, to determine whether these compounds have antioxidant properties and antimicrobial potential.
Methods: The polyphenolic extractions of the dried powdered samples have been performed using 80 % methanol. Total phenols and flavonoids concentrations were analyzed using Folin-Ciocalteu's and aluminum chloride methods. The antioxidant activity was investigated with three different methods: the β-carotene bleaching (BCB) test, the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)free radical scavenging method and the ferric reducing activity power (FRAP) assay. Polyphenols from the medicinal plants were screened for their antibacterial activities against pathogenic microorganisms (Staphylococcus aureus, Pseudomonas aeruginosa and Esherichia coli) using agar disc diffusion method and minimum inhibitory concentration.
Results: The total phenolic and flavonoids contents of the samples varied from (30.34±0.52) mg GAE/g extract to (602.71±2.01) mg GAE/g extract and from (16.61±0.04) mg QE/g extract to (21.91±0.31) mg QE/g extract, respectively. The antioxidant activity measurement, expressed as percentage inhibition of DPPH free radical, ranged from (44.00±0.06) % to (95.70±0.49) %. With further data analysis, it was found that there was a positive correlation between the total phenolic content of a given sample and its antioxidant activity (R2= 0.8064). The results showed that a remarkable inhibition of the bacterial growth was shown against the tested microorganisms. The phytochemicals analyses of the plants were carried out. The microbial activity of the plants extracts was due to the presence of various active compounds.
Conclusion: These results suggest that phenolic compounds in these plants provide substantial antioxidant and antibacterial activities which vary to a great extent. Hence, these plants can be used to discover bioactive natural products that may serve as leads for the development of new pharmaceuticals research activities.
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Benhammou N, Atik Bekkara F, Panovska TK. Antioxidant and antimicrobial activities of the Pistacialentiscus and Pistaciaatlantica extracts. Afr J Pharm Pharmacol 2008;2:22-8.
Halliwell B. Free radicals and antioxidants: a personal view. Nutr Rev 1994;52:253-65.
Praveen K, Ramamoorthy, Bono A. Antioxidant activity, total phenolic and flavonoid content of Morinda citrifolia fruit extracts from various extraction processes. J Eng Sci Technol 2007;2:70-80.
Viana H, Arruda M, Rainha N, Rosa J, Barreto M. In vitro toxicity and antioxidant activities of Hedychium gardnerianum from S. Miguel (Azores). Planta Med 2010;76:305-10.
Chandra S, Lata H, Varma A. Biotechnology for medicinal plants: Micropropagation and Improvement. New York: Springer; 2013.
Adwan G, Mhanna M. Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. J Sci Res 2008;3:134-9.
Noumedem J, Mihasan M, Lacmata S, Stefan M, Kuiate J, Kuete V. Antibacterial activities of the methanol extracts of ten Cameroonian vegetables against Gram-negative multidrug-resistant bacteria. BMC Complementary Altern Med 2013;13:1-9.
Naili MB, Alghazeer RO, Saleh NA, Al-Najjar AY. Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea). Arabian J Chem 2010;3:79-84.
Manna C, Galletti P, Cucciolla V, Montedoro G, Zappia V. Olive oil hydroxytyrosol protects human erytrocytes against oxidative damages. J Nutr Biochem 1999;10:159-65.
Servili M, Selvaggini R, Esposto S, Taticchi A, Montedoro G, Morozzi M. Health and sensory properties of virgin olive oil hydrophylic phenols: agronomic and technological aspects of production that affect their occurrence in the oil. J Chromatogr 2004;1054:113-27.
de la Torre-Carbort K, Jauregui O, Gimeno E, Castellote AI, Lamuela-Raventós RM, López-Sabater MC. Characterization and quantification of phenolic compounds in olive oils by solid-phase extraction, HPLC-DAD, and HPLC-MS/MS. J Agric Food Chem 2005;53:4331-40.
Benavente-Garcia O, Castillo J, Lorente J, Ortuno A, Del Rio JA. Antioxidant activity of phenolics extracted from Olea europaeaL. leaves. Food Chem 2000;68:457-62.
Soler RC, EspÃn JC, Wichers HJ. Review oleuropein and related compounds. J Sci Food Agric 2000;80:1013-23.
Amhamdi H, Aouinti F, Wathele JP, Elbachiri A. Chemical composition of the essential oil of Pistacia lentiscus L. from eastern morocco. Rec Nat Prod 2009;3:90-5.
Tyagi S, Chirag JS, Dhruv M, Ishita M, Gupta AK, Usman MRM, et al. Medical benefits of Apiumgraveolens (celery herb). J Drug Discovery Ther 2013;1:36-8.
Abdoul-Azize S, Bendahmanec BM, Hichamia A, Dramanea G, Simonina AM, Benammare C, et al. Effects of Zizyphus lotus L. (Desf.) polyphenols on Jurkat cell signaling and proliferation. Int Immuno pharmacol 2013;15:364-71.
Colle D, Arantes LP, Gubert P, Da Luz SC, Athayde ML, Teixeira Rocha JB, et al. Antioxidant properties of Taraxacumofficinale leaf extract are involved in the protective effect against hepatoxicity induced by Taraxacum officinale in mice. J Med Food 2012;15:549-56.
Lutsenko Y, Bylka W, Matławska I, Darmohray R. Hedera helix as a medicinal plant. Herba Pol 2010;56:83-96.
Rashed KNZ. Antioxidant activity of hedera helix L. extracts and the main phyto constituents. Int J Allied Med Sci Clin Res 2013;1:62-4.
Baananou S, Bouftira I, Amor M, Kamel B, Naceur AB. Antioxidant activity of Apium graveolens extracts. J Biol Act Prod Nat 2011;5:340-3.
Rauf A, Uddin G, Khan H, Siddiqui BS, Arfan M, Yousuf M, et al. Analgesic and antioxidant activity of crude extracts and isolated fractions of aerial parts of Hedera helix L. JSM Chem 2014;2:1012-6.
Silva S, Gomes L, Leitão F, Coelho AV, Vilas Boas L. Phenolic compounds and antioxidant activity of Olea europaea L. Fruits and Leaves. Food Sci Technol Int 2006;12:385-96.
Aouinti F, Zidane H, Tahri M, Wathelet JP, El Bachiri A. Chemical composition, mineral contents and antioxidant activity of fruits of Pistacialentiscus L. from eastern morocco. J Mater Environ Sci 2014;5:199-206.
Ivanov IG. Polyphenols content and antioxidant activities of Taraxacum officinale F. H. Wigg (Dandelion) Leaves. Int J Pharmacog Phytochem Res 2014;15:889-93.
Bakhtaoui FZ, Lakmichi H, Megraud F, Chait A, Gadhi CEA. Gastro-protective, anti-Helicobacter pylori and, antioxidant properties of Moroccan Zizyphus lotus L. J Appl Pharm Sci 2014;4:81-7.
Parr AJ, Bolwell GP. Phenols in plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profile. J Sci Food Agric 2000;80:985-1012.
Al-fartosy AJM. Antioxidant properties of a methanolic extract from Inula graveolens L. Turk J Agric For 2011;35:591-6.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nat 1958;29:1199-200.
Yen GC, Duh PD. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen species. J Agric Food Chem 1994;42:629-32.
Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 1996;239:70-6.
Kuli T, Dragovi-Uzelac V, Milos M. Antioxidant activity of aqueous tea infusions prepared from oregano, thyme, and wild thyme. Food Technol Biotechnol 2003;44:485-92.
Harborne JB. Phytochemical methods. London: Chapman and Hall; 1983.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol 1999;299:152-78.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555-9.
Akinsulire OR, Aibinu IE, Adenipekun T, Adelowotn T, Odugbemi T. In vitro antimicrobial activity of crude extracts from plants Bryophyllum pinnatum and Kalanchoe crenata. Afr J Tradit Complementary Altern Med 2007;4:338-44.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
Kang CG, Hah DS, Kim CH, Kim YH, Kim E, Kim JS. Evaluation of antimicrobial activity of the methanol extracts from 8 traditional medicinal plants. Toxicol Res 2011;27:31-6.
Siddhuraju P. The antioxidant activity and free radical scavenging capacity of phenolics of raw and dry heated moth bean (Vigna aconitifolia) (Jacq.) Marechal seed extracts. Food Chem 2006;99:149-57.
Gow-Chin Y, Pin-Der D. Antioxidative properties of methanolic extracts from peanus hulls. J Am Oil Chem Soc 1993;70:383-6.
Gow-Chin Y, Hui-Yin C. Antioxidant activity of various tea extracts in relation to their anti mutagenicity. J Agric Food Chem 2009;43:27-32.
Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing the power of edible wild mushrooms from the northeast portugal: individual cap and stipe activity. Food Chem 2007;100:1511-6.
Indu H, Seenivasan R. In vitro antioxidant activity of selected seaweeds from the southeast coast of India. Int J Pharm Pharm Sci 2013;5:474-84.
Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992;40:945-8.
Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Res 1995;22:375-83.
Wojdyło A, Oszmianski J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem 2007;105:940-5.
Tiveron AP, Melo PS, Bergamaschi KB, Vieira TMFS, Regitano-d’Arce MAB, Alencar SM. Antioxidant activity of brazilian vegetables and its relation with phenolic composition. Int J Mol Sci 2012;13:8943-57.
Fazal SF, Singla RK. Review on the pharmacognostic and pharmacological characterization of Apium Graveolens Linn. Indo Global J Pharm Sci 2012;2:36-42.
Mezni F1, Khouja ML, Gregoire S, Martine L, Khaldi A, Berdeaux O. Effect of growing area on tocopherols, carotenoids and fatty acid composition of Pistacia lentiscus edible oil. Nat Prod Res 2014;28:1225-30.
Binsan W, Benjakul S, Visessanguan W, Roytrakul S, Tanaka M, Kishimura H. Antioxidative activity of Mungoong, an extract paste, from the cephalothorax of white shrimp (Litopenaeusvannamei). Food Chem 2008;106:185–93.
Mau JL, Lin HC, Song SF. Antioxidant properties of several specialty mushrooms. Food Res Int 2002;35:519-26.
Cody V, Middleton E, Harborne J. Plant flavonoids in biology and medicine: biochemical, pharmacological and structure-activity relationships. New York: Liss AR; 1986.
Aruoma OL, Cuppett SL. Antioxidant methodology in vivo and in vitro Concepts. Illinois: AOCS Press Champaign; 1997.
Huang SJ, Mau JL. Antioxidant properties of methanolic extracts from Agaricusblazei with various doses of γ-irradiation. Food Sci Technol 2006;39:707-16.
Djeussi DE, Noumedem JAK, Seukep JA, Fankam AG, Voukeng IK, Tankeo SB, et al. Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria. BMC Complementary Altern Med 2013;13:1-8.
Uddin G, Rauf A, Qaisar M, Ur Rehman T, Latif A, Ali M. Preliminary phytochemical screening and antimicrobial activity of Hedera helix L. Middle-East J Sci Res 2011;8:198-202.
Mezni F, Aouadhi C, Khouja ML, Khaldi A, Maaroufi A. In vitro antimicrobial activity of Pistacia lentiscus L. edible oil and phenolic extract. Nat Prod Res 2015;29:565-70.
Naili MB, Alghazeer RO, Saleh NA, Al-Najjar AY. Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea). Arabian J Chem 2010;3:79–84.
Pereira AP, Ferreira IC, Marcelino F, Valentão P, Andrade PB, Seabra R, et al. Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrançosa) leaves. Mol 2007;26:1153-62.
Ahmed AM, Rabii NS, Garbaj AM, Abolghaita S. Antibacterial effect of olive (Olea europaea L.) leaves extract in raw peeled undeveined shrimp (Penaeus semisulcatus). Int J Veterinary Sci Med 2014;2:53-6.
Okoro IO, Osagie A, Asibor OE. Antioxidant and antimicrobial activities of polyphenols from ethnomedicinal plants of Nigeria. Afr J Biotechnol 2010;9:2989-93.