THE EFFECTS OF COSMOS CAUDATUS AND PIPER SARMENTOSUM AQUEOUS EXTRACTS ON MALE MICE FERTILITY
Keywords:Cosmos caudatus, Piper sarmentosum, Sperm quality, in-vivo fertilisation
Objective: This study was conducted to determine the sperm quality and fertility of male mice supplemented with Cosmos caudatus and Piper sarmentosum aqueous extracts.
Methods: The male mice were treated with either 0.2 ml of distilled water, 500 mg/kg bwt of Cosmos caudatus or Piper sarmentosum aqueous extract for the duration of four weeks. The body weight was checked on a weekly basis. Treated male mice then were mated with proven fertile females and the numbers of implantation sites were counted. At the end of the experimental duration, treated males were sacrificed by cervical dislocation and subjected to sperm analysis.
Results: Our data revealed that Cosmos caudatus and Piper sarmentosum increased the sperm quality and fertility in male mice. In addition, we found that the body weight was not affected by Cosmos caudatus or Piper sarmentosum aqueous extract.
Conclusion: In conclusion, Cosmos caudatus and Piper sarmentosum produced a remarkable increase in male mice reproductive performance.
Chauhan NS, Sharma V, Dixit VK, Thakur M. A review on plants used for the improvement of sexual performance and virility. BioMed Res Int 2014. doi. org/10.1155/2014/868062. [Epub 2014 Aug 18]
Ajayi AF, Jegede AI. Reproductive profile of male wistar rats treated with graded doses of aqueous extract of Titonia diversifolia leaf. World J Life Sci Med Res 2012;2:48-53.
Rewatkar KK, Shahzad N, Ahmed A, Khan MI, Ganesh N. A landmark approach to the aphrodisiac property of Abelmoschus manihot (L.). Int J Phytomed 2010;2:312-9.
Singh R, Ali A, Jeyabalan G, Semwal A. Current status of Indian medicinal plants with aphrodisiac potential. J Acute Disease 2013;2:13-21.
Mediani A, Abas F, Khatib A, Tan CP. Cosmos caudatus as a potential source of polyphenolic compounds: optimisation of oven drying conditions and characterisation of its functional properties. Molecules 2013;18:10452-64.
Rasdi NHM, Samah OA, Sule A, Ahmed QU. Antimicrobial studies of Cosmos caudatus Kunth. (Compositae). J Med Plants Res 2010;4:669-73.
Bodeker G. Health and beauty from the rainforest: Malaysian traditions of ramuan. Kuala Lumpur: Didier Millet; 2009.
Hassan WE. Healing herbs of Malaysia. Kuala Lumpur: Federal Land Development Agency; 2006.
Mohamed N, Khee SGS, Shuid AN, Muhammad N, Suhaimi F, Othman F, et al. The effects of Cosmos caudatus on structural bone histomorphometry in ovariectomized rats. J Evidence-Based Complementary Altern Med 2012. doi.org/10.1155/2012/817814. [Article in Press]
Booh MJ, Hashim M, Ismail NH, Daud D, Samsulrizal N, Yahya MFZR. Effects of Cosmos caudatus on sperm quality of mice, Mus musculus. Malays Appl Biol 2015;44:89-93.
Ugusman A, Zakaria Z, Chua K. Flavanoids of Piper sarmentosum and its cytoprotective effects against oxidative stress. EXCLI J 2012;11:705-14.
Atiax E, Ahmad F, Sirat HM, Arbain D. Antibacterial activity and cytotoxicity screening of Sumatran kaduk (Piper sarmentosum Roxb). Iran J Pharmacol Ther 2011;10:1-5.
Peungvicha P, Thirawarapan SS, Temsiririkkul R, Wanabe H, Kumar-Prasain J, Kadota S. Hypoglycemic effect of water extract of Piper sarmentosum leaves in rats. J Ethnopharm 1998;60:27-32.
Najib NA, Rehman N, Furuta T, Kojima S, Takane K, Ali MM. Antimalarial activity of extracts of Malaysian medicinal plants. J Ethnopharmacol 1999;64:249-54.
Ong HC, Nordiana M. Malay ethno-medico botany in Machang, Kelantan, Malaysia. Fitoterapia 1999;70:502-13.
Ong HC, Norzalina J. Malay herbal medicine in Gemenceh, Negeri Sembilan, Malaysia. Fitoterapia 1999;70:10-4.
Parhizkar S, Che-Zainudin CZB, Dollah MA. Effect of Phaleria macrocarpa on sexual function of rats. Avicenna J Phytomed 2013;3:371-7.
Cammack KM, Antoniou E, Hearne L, Lamberson WR. Testicular gene expression in male mice divergent for fertility after heat stress. Theriogenology 2009;71:651-61.
NAFA manual on basic semen analysis. NAFA and ESHRE-SIGA; 2002.
Agarwal A, Sekhon LH. The role of antioxidant therapy in the treatment of male infertility. Human Fertility 2010;13:217-25.
Hafizah A, Zaiton Z, Zulkhairi A, Mohd-Ilham A, Nor-Anita M, Zaleha A. Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide. J Zhejiang Univ Sci 2010;11:357-65.
Radman HM, Yusof K, Saad QHM, Wan-Ngah WZ, Abdullah A. The effect of Ulam Raja (Cosmos caudatus) on drug metabolizing enzymes, lipid peroxidation and antioxidant status in mice liver. Int J PharmTech Res 2014;6:1213-25.
Mbongue FGY, Kamtchouing P, Essame OJL, Yewah PM, Dimo T, Lontsi D. Effect of the aqueous extract of dry fruits of Piper guineense on the reproductive function of adult male rats. Indian J Pharmacol 2005;37:30-2.