MICROWAVE-ASSISTED SYNTHESIS OF AGNP USING AQUEOUS LEAVES EXTRACT OF VINCA ROSEA AND ITS THERAPEUTIC APPLICATION
Keywords:Vinca, Anti-cancer, Silver nanoparticles
Objective: Green synthesis of silver nanoparticles was attempted with the help of aqueous Vinca rosea leaf extract. The aim of the study was to combine the therapeutic activity of Vinca rosea and the deep tissue penetration capabilities of the silver nanoparticles.
Methods: This study focuses on the green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of Vinca rosea leaves, its characterization, and evaluation of its antibacterial and anticancer activity by diffusion method and MTT assay using human lung carcinoma cell line A549 respectively. The nanoparticles were synthesised by exposing the reaction mixture containing silver nitrate and Vinca leaf aqueous extract to microwave radiation.
Results: The characterization of synthesised nanoparticles was carried out by observing the peaks on scanning from 250 to 800 nm using UV spectroscopy, the end point for the complete formation of nanoparticles marked by a colour change to reddish brown. Dynamic Light Scattering (DLS) which evaluated particle size uniformity and Scanning Electron Microscopy (SEM) which determines the particle size revealed that the nanoparticles were spherical in shape and measured an average of 50.75 nm. 170Âµg/ml of AgNPs of Vinca leaf aqueous extract should potent anti-bacterial activity tested by agar well diffusion method as well as the cytotoxic activity which was evaluated by MTT assay.
Conclusion: The synthesised nanoparticles were found to be potentially cytotoxic against A549 cell line and also demonstrated anti-bacterial activity. The activity may be attributed to the fact that silver ions are known to impair macromolecules containing sulphur and phosphorus like protein and DNA owing to their small size and high penetration power.
Abou El-Nour KMM, Eftaiha A, Al-Warthan A, Ammar RAA. Synthesis and application of silver nanoparticles. Arabian J Chem 2010;3:135-40.
Waren CW, Nie S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 1998;281:2016-8.
Vaseashta A, Dimova-Malinovska D. Nanostructured and nanoscale devices, sensors and detectors. Sci Technol Adv Mater 2005;6:312-8.
Langer R. Drug delivery. Drugs on target. Science 2001;293:58-9.
Farokhzad OC, Cheng J, Teply BA, Sherifi I, Jon S, Kantoff PW, et al. Targeted nanoparticle-aptamer bioconjugates for cancer chemotherapy in vivo. Proc Natl Acad Sci USA 2006;103:6315-20.
Garg S. Microwave-assisted rapid green synthesis of silver nanoparticles using Saraca indica leaf extract and their antibacterial potential. Int J Pharma Sci Res 2013;4:3615-9.
Ozin GA. Nanochemistry: synthesis in diminishing dimensions. Adv Mater 1992;4:612â€“49.
Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 2009;145:83-96.
Garg S, Chandra A, Mazumdar A, Mazumdar R. Analgesic potential of hydrogels of silver nanoparticles using aqueous extract of Saraca indica bark. Int J Pharma Sci Res 2014;5:240-5.
GuzmÃ¡n MG, Dille J, Godet S. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biomol Eng 2009;2:104-11.
Kim YH, Lee DK, Kang YS. Synthesis and characterization of Ag and Ag-SiO2 nanoparticles. Colloids Surf A 2005;257â€“258:273-6.
Bae CH, Nam SH, Park SM. Formation of silver nanoparticles by laser ablation of a silver target in NaCl solution. Appl Surf Sci 2002;197-198:628-34.
Patel K, Kapoor S, Dave DP, Mukherjee T. Synthesis of Pt, Pd, Pt/Ag and Pd/Ag nanoparticles by the microwave-polyol method. J Chem Sci 2007;117:311-5.
Zhang JP, Chen P, Sun CH, Hu X. Sonochemical synthesis of colloidal silver catalysts for the reduction of complexing silver in DTR system. Appl Catal A 2004;266:49-54.
Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, et al. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B 2003;28:313-8.
Basavaraja S, Balaji DS, Arunkumar L, Rajasab AH, Venkataraman A. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Mater Res Bull 2008;43:1164â€“70.
Raghunandan D, Mahesh BD, Basavaraja S, Balaji SD, Manjunath SY, Venkataraman A. Microwave-assisted rapid extracellular synthesis of stable bio-functionalized silver nanoparticles from guava (Psidium guajava) leaf extract. J Nanopart Res 2011;13:2021-8.
Garg S, Chandra A, Mazumdar A, Mazumdar R. Green synthesis of silver nanoparticles using Arnebia nobilis root extract and wound healing potential of its hydrogel. Asian J Pharm 2014;8:95-101.
Saxena A, Tripathi RM, Singh RP. Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Digest J Nanomater Biostructures 2010;5:427-32.
Kumar V, Yadav SC, Yadav SK. Syzygium cumini leaf and seed extract mediated biosynthesis of silver nanoparticles and their characterization. J Chem Technol Biotechnol 2010;85:1301-9.
Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, et al. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B 2010;81:81-6.
Bankara AV, Joshi BS, Kumar AR, Zinjardea SS. Banana peel extract mediated synthesis of gold nanoparticles. Colloids Surf B 2010;80:45-50.
Garg S. Rapid biogenic synthesis of silver nanoparticles using black pepper (Piper nigrum) corn extract. Int J Innovations Biol Chem Sci 2012;3:5-10.
Devi JS, Bhimba BV, Ratnam K. In vitro anticancer activity of silver nanoparticles synthesized using the extract of Gelidiella Sp. Int J Pharm Pharm Sci 2012;4 Suppl 4:710-5.
Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using Aloevera plant extract. Biotechnol Prog 2006;22:577-83.
Shawkey AM, Rabeh MA, Abdulall AK, Abdellatif OF. Green nanotechnology: Anticancer activity of silver nanoparticles using Citrullus colocynthis aqueous extracts. Adv Life Sci Technol 2013;13:60-70.
Devi JS, Bhimba BV. Anticancer activity of silver nanoparticles synthesised by the seaweed Ulva lactuca invitro. Sci Rep 2012;1:242-6.
Zolghadri S, Saboury A, Golestani A, Divasalar A, Rezaei-Zarchi S, Moosavi-Moovahedi A. Interaction between the silver nanoparticle and bovine hemoglobin at different temperatures. J Nanopart Res 2009;11:1751-8.
Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, et al. The bacterial effect of silver nanoparticles. Nanotechnology 2005;16:2346-53.