ESTABLISHMENT OF PSEUDERANTHEMUM PALATIFERUM (NEES) RADLK CALLUS CULTURE AND SCREENING OF SECONDARY METABOLITE PRODUCTION
Keywords:
Pseuderanthemum palatiferum, plant tissue culture, callus, secondary metabolites, thin-layer chromatographyAbstract
Objective: This study aims to establish callus culture of Pseuderanthemum palatiferum and to investigate the production of secondary metabolites from callus extracts.
Methods: Callus tissues were initiated using explants from in vitro, aseptically-grown plants. The effects of medium salt base (Murashige & Skoog; MS or Gamborg B5; GB5) and plant growth regulators (2,4-dichlorophenoxyacetic acid; 2,4-D, naphthaleneacetic acid; NAA, benzyl aminopurine; BAP) on the initiation of callus tissues were investigated. The growth of callus culture was studied, and an optimized medium was determined. The production of secondary metabolites in callus was investigated, in comparison with P. palatiferum leaf, on ethanolic extracts using test reagents and thin-layer chromatography (TLC).
Results: The condition suitable for initiation of callus from leaf explant was MS salt bases, supplemented with 5.37 µM NAA, which yielded friable callus within 2 w. After transfer, best growth was observed in MS medium supplemented with 5.37 µM NAA and 0.44 µM BAP, after 4 w. Chemical screening and TLC analysis of callus extracts showed presences of some secondary metabolites similar to that of the leaf extract, together with additional phytochemicals not originally found in P. palatiferum plant.
Conclusion: Callus culture was successfully established. With optimum culture conditions, this in vitro culture and can serve as another method to obtain medicinally-useful secondary compounds from P. palatiferum.
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Dieu HK, Loc CB, Yamasaki S, Hirata Y. The ethnobotanical and botanical study on Pseuderanthemum palatiferum as a new medicinal plant in the mekong delta of vietnam. Jpn Agric Res Q 2005;39:191-6.
Chayarop K, Peungvicha P, Wongkrajang Y, Chuakul W, Amnuoypol S, Temsiririrkkul R. Pharmacognostic and phytochemical investigations of Pseuderanthemum palatiferum (Nees) Radlk. ex Lindau leaves. Pharmacogn J 2011;3:18-23.
Padee P, Nualkaew S, Talubmook C, Sakuljaitrong S. Hypoglycemic effect of a leaf extract of Pseuderanthemum palatiferum (Nees) Radlk. in normal and streptozotocin-induced diabetic rats. J Ethnopharmacol 2010;132:491-6.
Sharma SN, Jha Z. Production of andrographolide from callus and cell suspension culture of Andrographis paniculata. J Cell Tissue Res 2012;12:3423-9.
Bhambhani S, Karwasara V, Dixit V, Banerjee S. Enhanced production of vasicine in Adhatoda vasica (L.) Nees. cell culture by elicitation. Acta Physiol Plant 2012;34:1571-8.
Panichayupakaranant P, Meerungrueang W. Effect of medium composition and light on root and rhinacanthin formation in Rhinacanthus nasutus cultures. Pharm Biol 2010;48:1192-7.
Vo TN, Nguyen PL, Tuong LT, Vo PN, Nguyen KPP, Nguyen NS. Constituents of the leaves of Pseuderanthemum carruthersii (Seem.) Guill. var. atropurpureum (Bull.) Fosb. Phytochem Lett 2012;5:673-6.
Vo TN, Nguyen PL, Tuong LT, Pratt LM, Vo PN, Nguyen KPP, et al. Lignans and triterpenes from the root of Pseuderanthemum carruthersii var. atropurpureum. Chem Pharm Bull 2012;60:1125-33.
Ragasa CY, Yumul J, Guanzon J, Rideouf JA. Antimicrobial triterpenes and sterols from Pseuderanthemum bicolor. Kimika 2001;17:1-4.
Kasmiyati S, Atmajaningrum F, Karwur dfF. The effect of 2,4-D to the callus growth of Pseuderanthemum acuminatissimum. J Ilmu Pertanian 2007;19:68-75.
Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1966;55:225-76.
Agastian P, Williams L, Ignacimuthu S. In vitro propagation of Justicia gendarussa Burm. f.–A medicinal plant. Indian J Biotechnol 2006;5:246-8.
Vidyalakshmi A, Ananthi S. Induction of andrographolide, A biologically active ingredient in callus of Andrographis paniculata (Burm. F) Wallich Ex. Nees. Bioeng Biosci 2013;1:1-4.
Arya D, Patni V, Kant U. In vitro propagation and quercetin quantification in callus cultures of Rasna (Pluchea lanceolata Oliver & Hiern.). Indian J Biotechnol 2008;7:383-7.
Jones AMP, Saxena PK. Inhibition of phenylpropanoid biosynthesis in Artemisia annua L.: A novel approach to reduce oxidative browning in plant tissue culture. PLoS One 2013;8:e76802.
Tamilselvan V, Rajeswari M. Impact of growth regulators on callus production of Asystasia gangetica (L) T. Anderson. Adv Appl Sci Res 2014;5:328-33.
Ali M, Abbasi BH, Ihsan ul h. Production of commercially important secondary metabolites and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Ind Crops Prod 2013;49:400-6.
Patra A, Rai B, Rout GR, Das P. Successful plant regeneration from callus cultures of Centella asiatica (Linn.) Urban. Plant Growth Regul 1998;24:13-6.
Moin S, Babu SS, Mahalakshmipriya A. In vitro callus production and antibacterial activity of Barleria lupulina Lindl. Asia Pac J Mol Biol Biotechnol 2012;20:59-64.
Umesh TG, Dilkalal A. Evaluation of antioxidant potential and reducing power of callus induced from leave of Asystasia gangetica (L.) T. Anderson. Int J Pharm Pharm Sci 2014;6:532-8.
Ramachandra Rao S, Ravishankar GA. Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 2002;20:101-53.
Salinas R, Arellano-GarcÃa J, Perea-Arango I, Ãlvarez L, Garduño-RamÃrez M, Marquina S, et al. Production of the anti-inflammatory compound 6-O-Palmitoyl-3-O-β-D-glucopyranosylcampesterol by callus cultures of Lopezia racemosa Cav. (Onagraceae). Molecules 2014;19:8679.
Hutzler P, Fischbach R, Heller W, Jungblut TP, Reuber S, Schmitz R, et al. Tissue localization of phenolic compounds in plants by confocal laser scanning microscopy. J Exp Bot 1998;49:953-65.
Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Hendrickson Publishers; 1996.
Shinde AN, Malpathak N, Fulzele DP. Determination of isoflavone content and antioxidant activity in Psoralea corylifolia L. callus cultures. Food Chem 2010;118:128-32.
Wang J, Xiao X, Wang Q, Li X, Zhang L, Li J. Accumulation of flavonoids and antioxidant activity of Stellera chamaejasme by an efficient callus culture. Hortic Environ Biotechnol 2013;54:441-9.
Sy L-K, Brown GD. Coniferaldehyde derivatives from tissue culture of Artemisia annua and Tanacetum parthenium. Phytochem 1999;50:781-5.