• K. Chawawisit School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat 80161, Thailand
  • P. Bhoopong Walailak University
  • W. Phupong Walailak University
  • M. Lertcanawanichakul Walailak University


Antimicrobial activity, Cytotoxic activity, Bioactive compounds, Streptomycessp KB1


Objective: To investigate the antimicrobial and cytotoxic activities of bioactive compounds produced by Streptomyces sp. KB1 isolated from the air sample at Ao-nang, Krabi province, Thailand.

Methods: Streptomyces sp. KB1 was identified by 16S rDNA gene sequencing. It was preliminarily investigated the antibacterial activity by cross streak method and then cultivated in the half-formula of Luria Bertani at 30 °C, 200 rpm in shaking incubator for 7 d. Culture broth of each day was prepared as cell-free culture broth and tested the anti-Staphylococcus aureus TISTR 517 activity by agar well diffusion method along with determined the protein concentration by Bradford assay. Bioactive compounds were assessed the antimicrobial activity and cytotoxicity against brine shrimp by broth microdilution method and brine shrimp lethality bioassay, respectively.

Results: Bioactive compounds were excreted into liquid culture medium within 2 d of cultivation and showed a broad spectrum of antibacterial activity. Whereas, the highest activity has shown since day 4th of an incubation period which had protein concentration equal 254 ± 3.1 µg/ml. Bioactive compounds showed strong activity against Gram-positive bacteria, moderate activity against Gram-negative bacteria and weak activity against yeasts and Pseudomonas aeruginosa TISTR 1467. The MIC, MBC or MFC, and LC50 value of bioactive compounds were in the range of 0.49-63.50, 0.49-127.00, and 131.58 µg/ml, respectively.

Conclusion: This study implied that bioactive compounds produced by Streptomyces sp. KB1 may be the important polypeptide compound that can be used as lead compounds for novel drug discovery.




Download data is not yet available.


Berdy J. Bioactive microbial metabolites. J Antibiot 2005;58:1-26.

Raja A, Prabakarana P. Actinomycetes and drug-an overview. Am J Drug Discovery Dev 2011;1:75-84.

Watve MG, Tickoo R, Jog MM, Bhole BD. How many antibiotics are produced by the genus Streptomyces? J Arch Microbiol 2001;176:386-90.

Rahman MA, Islam MZ, Khondkar P, Islam MA. Characterization and antimicrobial activities of a polypeptide antibiotic isolated from a new strain of Streptomyces parvulus. J Bangladesh Pharm 2010;13:14-6.

Manasa M, Poornima G, Abhipsa V, Rekha C, Prashith KTR, Onkarappa R, et al. Antimicrobial and antioxidant potential of Streptomyces sp. RAMPP 065 isolated from kudremukh soil, Karnataka, India. Sci Technol Arts Res J 2012;1:39-44.

Nandhini SU, Selvam MM. Antimicrobial compounds produced by Streptomyces strain. Int J Pharm Pharm Sci 2013;5:176-8.

Procopio REL, Silva IR, Martins MK, Azevedo JL, Araujo JM. Antibiotics produced by Streptomyces. Braz J Infect Dis 2012;16:466-71.

Lertcanawanichakul M, Sawangnop S. A comparison of two methods used for measuring the antagonistic activity of Bacillus Species. Walailak J Sci & Tech 2008;5:161-71.

CLSI. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute; 2006. p. M100-S16.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. J Anal Biochem 1976;72:248-54.

Yilmaz MT. Minimum inhibitory and minimum bactericidal concentrations of boron compounds against several bacterial strains. Turk J Med Sci 2012;42:1423-9.

Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD. A micro-well cytotoxicity assay using Artemia salina (Brine Shrimp). J Plant Med 1992;59:250-2.

Ahmad SJ, Lian HH, Basri DF, Zin NM. Mode of action of endophytic Streptomyces sp. SUK 25 extract against MRSA; Microscopic, Biochemical and Time-kill analysis. Int J Pham Sci Rev Res 2015;30:11-7.

Lertcanawanichakul M. Isolation, phylogenetic characterization and antibacterial activity of Streptomyces strains isolated from the air at Walailak University. Int J Pharma Bio Sci 2015;6:1089-100.

Hasani A, Kariminik A, Issazadeh K. Streptomycetes: characteristics and their antimicrobial activities. Int J Adv Biol Biomed Res 2014;2:63-75.

Lertcanawanichakul M, Pondet K, Kwantep J. In vitro antimicrobial and antioxidant activities of bioactive compounds (secondary metabolites) extracted from Streptomyces lydicus A2. J Appl Pharm Sci 2015;5:17-21.

Lorente A, Makowski K, Albericio F, Alvarez M. Bioactive marine polyketides as potential and promising drugs. Ann Marine Biol Res 2014;1:1-10.

Shetty PR, Buddana SK, Tatipamula VB, Naga YVV, Ahmad J. Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity. Braz J Microbiol 2014;45:303-12.



How to Cite

Chawawisit, K., P. Bhoopong, W. Phupong, and M. Lertcanawanichakul. “ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF BIOACTIVE COMPOUNDS PRODUCED BY STREPTOMYCES SP. KB1”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 118-22,



Original Article(s)