ISOLATION, SCREENING AND CHARACTERIZATION OF L-ASPARAGINASE PRODUCING FUNGI FROM MEDICINAL PLANTS
Keywords:L-asparaginase, Fusarium sp Papaya leaves, Submerged fermentation
Objective: To isolate and characterize of L-asparaginase producing fungi from medicinal plants.
Methods: Fungal strains were isolated on standard media, characterized and were screened for their ability to produce L-asparginase, used in the treatment of certain types of cancers, using modified Czapek-Dox medium supplemented with L-asparagine.
Results: A total of 10 fungal isolates were obtained from 3 plant leaf samples and two isolates exhibited significant L-aspargniase production. Fusarium sp. (SMGR-F1) isolated from the papaya leaves showed the maximum activity. The organism was grown under submerged fermentation conditions at 30 Â°C produced 111.07Â±1.53 IU/ml of L-asparaginase at 120 h.
Conclusion: L-asparaginase is being effectively used for the treatment of acute lymphoblastic leukemia and tumor cells. Fusarium sp. was isolated from the papaya plant leaves showing significant L-asparaginase activity and thus can be further exploited for the commercial production of L-asparaginase.
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