• Ram Kumar Gulbarga University
  • Vijay Kumar Sedolkar Gulbarga University
  • A. G Triveni Gulbarga University
  • Mendem Suresh Kumar Gulbarga University
  • Channappa T. Shivannavar Gulbarga University
  • Subhaschandra M. Gaddad Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Kalaburagi, Karnataka 585106, India


L-asparaginase, Fusarium sp Papaya leaves, Submerged fermentation


Objective: To isolate and characterize of L-asparaginase producing fungi from medicinal plants.

Methods: Fungal strains were isolated on standard media, characterized and were screened for their ability to produce L-asparginase, used in the treatment of certain types of cancers, using modified Czapek-Dox medium supplemented with L-asparagine.

Results: A total of 10 fungal isolates were obtained from 3 plant leaf samples and two isolates exhibited significant L-aspargniase production. Fusarium sp. (SMGR-F1) isolated from the papaya leaves showed the maximum activity. The organism was grown under submerged fermentation conditions at 30 °C produced 111.07±1.53 IU/ml of L-asparaginase at 120 h.

Conclusion: L-asparaginase is being effectively used for the treatment of acute lymphoblastic leukemia and tumor cells. Fusarium sp. was isolated from the papaya plant leaves showing significant L-asparaginase activity and thus can be further exploited for the commercial production of L-asparaginase.



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Author Biography

Subhaschandra M. Gaddad, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Kalaburagi, Karnataka 585106, India

Department of Microbiology,



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How to Cite

Kumar, R., V. K. Sedolkar, A. G. Triveni, M. S. Kumar, C. T. Shivannavar, and S. M. Gaddad. “ISOLATION, SCREENING AND CHARACTERIZATION OF L-ASPARAGINASE PRODUCING FUNGI FROM MEDICINAL PLANTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 1, Jan. 2016, pp. 281-3,



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