GC-MS ANALYSIS OF METHANOLIC EXTRACT OF LEAVES OF RHODODENDRON CAMPANULATUM
Keywords:Rhododendron campanulatum, Gas Chromatography-Mass Spectrometry, Phytochemical compounds
Objective: Rhododendron campanulatum is a native of high altitude and is known for its medicinal properties. The present study is aimed to identify the phytochemical constituents in the leaf extract of R. campanulatum using Gas Chromatography and Mass Spectroscopy (GC-MS).
Methods: The methanolic leaf extract was prepared using Accelerated Solvent Extraction system at room temperature and high pressure. Phytochemical screening of methanolic extract of R. campanulatum was performed using GCMS-QP2010 Plus (Shimadzu, Kyoto, Japan) and the spectrum was interpreted on the basis of the databases of National Institute Standard and Technology (NIST11LIB) and WILEY8LIB.
Results: The GC-MS analysis revealed the presence of 49 phytochemical compounds in the methanolic leaf extract. Baccharis oxide (9.99%), betuligenol (8%), alpha and beta-amyrin (7.38 and 2.64%), geranyl acetate (5.91%), (R)-(-)-14-methyl-8-hexadecyn-1-ol (5.19%) and phthalic acid (5.16%) were identified as major constituents.
Conclusion: The methanolic leaf extract of R. campanulatum contains various phyto-compounds of pharmaceutical and industrial importance.
Chamberlain DF. The genus Rhododendron, its classification and synonymy. Chamberlain DF. editor. Royal Botanic Gardens, Kew; 1996.
Sekar KC, Srivastava SK. Rhododendrons in Indian Himalayan region: diversity and conservation. Am J Plant Sci 2010;1:131-7.
Bhattacharyya D. Rhododendron species and their uses with special reference to the Himalayas. Assam University J Sci Technol: Biol Environ Sci 2011;7:161-7.
Popescu R, Kopp B. The genus Rhododendron: an ethnopharmacological and toxicological review. J Ethnopharmacol 2013;147:42-62.
Pushpangadan P, Nyuman U, George V. Glimpses of Indian ethnopharmacology. Pushpangadan P. Editor. Visual Security Printing Enterprise pvt. Ltd, New Delhi; 1996. p. 123.
Prakash D, Upadhyay G, Singh BN, Dhakarey R, Kumar S, Singh KK. Free-radical scavenging activities of Himalayan Rhododendrons. Curr Sci India 2007;92:526-32.
Kunwar MR, Shresta KP, Bussmann RW. Traditional herbal medicine in far-west Nepal: a pharmacological appraisal. J Ethnobiol Ethnomed 2010;6:2-18.
Paudel A, Panthee S, Shakya S, Amatya S, Shresta TM, Amatya AM. Phytochemical and antibacterial properties of Rhododendron campanulatum from Nepal. J Tradit Med 2011;6:252-8.
Tantry MA, Khan R, Akbar S, Dar AR, Shawl AS, Alam MS. An unusal bioactive oleanane triterpenoid from Rhododdendron campanulatum D. Don. Chin Chem Lett 2011;22:575-9.
Sharma N, Sharma UK, Gupta AP, Sinha AK. Simultaneous determination of epicatechin, syringic acid, quercetin-3-Ogalactosideand quercitrin in the leaves of Rhododendron species by using a validated HPTLC method. J Food Compos Anal 2010;23:214â€“9.
Mamta, Mehrotra S, Amitabh, Kirar V, Vats P, Nandi SP, et al. Phytochemical and antimicrobial activities of Himalayan Cordyceps sinensis (Berk.) Sacc. Indian J Exp Biol 2015;53:36-43.
Shibuya M, Sagara A, Saitoh A, Kushiro T, Ebizuka Y. "Biosynthesis of baccharis oxide, a triterpene with a 3, 10-oxide bridge in the A-ring". Org Lett 2008;10:5071â€“4.
Sasaki M, Kondo M, Sato K, Umeda M, Kawabata K, Takahashi Y, et al. Rhododendrol, a depigmentation-inducing phenolic compound, exerts melanocyte cytotoxicity via a tyrosinase-dependent mechanism. Pigm Cell Melanoma Res 2014;27:754-63.
Seigler DS. Triterpenes and Steroids. Seigler DS. editor. Plant secondary Metabolism. 1st ed. US, Springer; 1998. p. 427-55.
Vazquez LH, Palazon J, Nocana A. The Pentacyclic Triterpenes Î±Î²-amyrins: A Review of Sources and Biological Activities. V Rao. Ed. Phytochemicals: a global perspective of their role in nutritional health. In Tech; 2012.
Lucindo QJ, Moreira JCF, Pasquali MA, Rabie SMS, Pires AS, SchrÃ¶der R, et al. Antinociceptive activity and redox profile of the monoterpenes (+)-Camphene, p-Cymene, and Geranyl Acetate in Experimental Models. ISRN Toxicology 2013;1-11. doi.org/10.1155/2013/459530. [Article in Press]
Fragrance raw materials monograph Geranyl acetate. Food Cosmet Toxicol 1974. p. 885.
De souse DP, Goncalves JCR, Junior LQ, Gcuz JS, Araujo M, Dealmeida RN. Study of the anticonvulsant effect of citronellol, a monoterpene alcohol, in rodents. Neurosci Lett 2006;401:231-5.
Park SN, Lim YK, Freire MO, Cho E, Jin D, Kook JK. Antimicrobial effect of linalool and Î±-terpineol against periodontopathic and cariogenic bacteria. Anaerobe 2012;18:369-72.
Peana AT, Aquila PS, Panin F, Serra G, Pippia P, Moretti AD. Anti-inflammatory activity of linalool and linayl acetate constituents of essential oils. Phytomedicine 2002;9:721-6.
Carnesecchi S, Schneider Y, Ceraline J, Duranton B, Gosse F, Seiler N, et al. Geraniol a component of plant essential oils, inhibits growth and polyamine biosynthesis in human colon cancer cells. J Pharma Col Exp Ther 2001;298:197-200.
Lorz PM, Towae FK, Enke W, JÃ¤ckh R, Bhargava N, Hillesheim W. Phthalic acid and derivatives. Ullmann's Encyclopedia of Industrial Chemistry, Wiley-VCH, Weinheim; 2007.