THE EFFECT OF PROPHYLACTIC INHIBITION OF INDUCIBLE NITRIC OXIDE SYNTHASE BY AMINOGUANIDINEON SERUM LEVELS OF SOME ADIPOCYTOKINES IN PRISTANE-INDUCED ARTHRITISIN RATS
Keywords:
Adipocytokines, Aminoguanidine, iNOS, Pristane, Rheumatoid ArthritisAbstract
Objective: The aim of the present study was to evaluate the effect of prophylactic inhibition of inducible nitric oxide synthase (iNOS) by aminoguanidine (AG) on serum levels of some adipocytokines in pristane-induced arthritis in rats.
Methods: Forty white Albino rats were divided randomly into four groups; each group was composed of ten rats (five male and five female). Group I (AP) has received AG (100 mg/kg/day) i. p. for seven days, and then at day 8, has received single 150 µl pristane dose sc. at the base of rat's tail. Group II (PC) has only received single 150 µl pristane sc. at the base of rat's tail, at day 8 from the start of the experiment. Group III (AC) has only received AG (100 mg/kg/day)i. p. for seven days. Group VI (VC) has only received normal saline via i. p. injection for seven days. At the end of the experiment time, rats were sacrificed, and serum samples were obtained and used for the measurement of iNOS, rheumatoid factor (RF), c-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukine-6 (IL-6), leptin and adiponectin levels, using the corresponding rat ELISA kits.
Results: Administration of pristane for the induction of RA has resulted in significant increase in all of the measured parameters in PC group as compared to VC and AC groups, except for iNOS where the increase was significant as compared to AC group only. Serum levels of RF, CRP and IL-6 in AP group have showed to be significantly elevated as compared to VC and AC groups. Administration of AG has resulted in different levels of reduction in all of the measured parameters in AP group as compared to PC group. The reduction was statistically significant with regard to CRP and leptin.
Conclusion: prophylactic administration of the selective iNOS inhibitor AG, has resulted in a reduction in serum levels of the measured adipocytokines which may reflect a reduction in the severity of PIA.
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