CYTOTOXIC EFFECT OF CRUDE EXTRACT AND FRACTION FROM CALOTROPIS GIGANTEA LEAVES ON HUMAN COLON CANCER WIDR CELL LINES

Roihatul Mutiah; Sukardiman .; Aty Widyawaruyanti

doi:10.22159/ijpps.2017v9i1.9022

Authors

Roihatul Mutiah Graduate Program, Faculty of Pharmacy, Airlangga University, Surabaya Indonesia
Sukardiman . Departement of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya Indonesia
Aty Widyawaruyanti Departement of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya Indonesia

DOI:

https://doi.org/10.22159/ijpps.2017v9i1.9022

Keywords:

Cytotoxic, Calotropis gigantea, WiDr cells, Colon cancer

Abstract

Objectives: This paper sought to understand and determine the cytotoxic's effects of crude extract and its fraction from Calotropis gigantea leaves on human colon cancer WiDr cell lines.

Methods: The ethanolic extract was fractionated gradually with certain substances to yield four fractions. The substances were dichloromethane, ethyl acetate, and butanol. The four fractions resulted in dichloromethane fraction, ethyl acetate fraction, butanol fraction, and a water fraction. These fractions were then investigated for their cytotoxic effects on WiDr cells. The cell viability was assessed using MTT colorimetric assay.

Results: The result indicated that the cytotoxic effects of the ethanolic extract (IC₅₀48.5 Î¼g/ml), ethyl acetate fraction (IC₅₀41.79 Î¼g/ml), and dichloromethane fraction (IC₅₀40.57Î¼g/ml) produced a much more potent effect than the butanol fraction (IC₅₀ 737.74 Î¼g/ml) and water fraction (IC₅₀8493 Î¼g/ml).

Conclusion: The ethanolic extract, ethyl acetate fraction and dichloromethane fraction exhibited a potent cytotoxic effect on human colon cancer WiDr cell line. The crude extract and fractions are potential to be developed as an anticancer agent in colon cancer therapy.

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