MICROMORPHOLOGICAL CHARACTERIZATIONS AND PHYTOCHEMICAL CONTENTS OF SOME PHLOMIS SPECIES FROM IRAN
Keywords:
Phlomis, Microscopic characterization, Phytochemical contents, Free radical scavengingAbstract
Objective: Microscopic characterization of a plant is a valuable method for accurate identification of the plant powder. The plants of Phlomis genus (Lamiaceae) are mostly distributed in the north and west of Iran with about 10 endemic species. In the present investigation, microscopic characterization of some Phlomis species including Phlomis bruguieri, Phlomis rigida, Phlomis kurdica, and Phlomis olivieri were assessed along with their phytochemical contents.
Methods: The powders of the mentioned plants were analyzed using Zeiss microscope attached to a digital camera. Phytochemical contents of the plants extracts including total phenol, tannin, and polysaccharide were measured as well as a radical scavenging activity using the 2,2-diphenyl-1-picryl-hydrazyl method.
Results: The results of this study indicated that diacytic stomata, glandular trichome, stellate trichome, and crystals were the characteristic features of the examined species. Total phenol, tannins, and polysaccharides of the plants were assessed ranging 66.0-101.8 µg gallic acid equivalent in mg dry extract (µg GAE/mg EXT), 6.9-9.5 µg tannic acid equivalent in mg dry extract (TAE/mg EXT), and 512-559 µg glucose equivalent in mg dry extract (GE/mg EXT), respectively. Moreover, half maximal inhibitory concentration (IC50) values of radical scavenging activity of the extracts were calculated according to the plot of inhibition percentage against different concentrations of each extract as 218.6, 112.0, 113.3, and 58.7 µg/ml, respectively.
Conclusion: The observed differences between Phlomis species can be applied in the accurate identification of these medicinal plants particularly in dried powdered materials regarding their microscopic characterizations and phytochemical contents.
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Jafari S, Saeidnia S, Shams Ardekani MR, Hadjiakhondi A, Khanavi M. Micromorphological and preliminary phytochemical studies of Azadirachta indica and Melia azedarach. Turk J Bot 2013;37:690-7.
Upton R, Graff A, Jolliffo G, Longer R, Williamson E. American Herbal Pharmacopoeia: Botanical Pharmacognosy, Microscopic Characterization of Botanical Medicines. London: CRC press; 2011.
Saeidnia S, Gohari AR. Pharmacognosy and molecular pharmacognosy in practice. Saarbrucken: LAP Lambert Academic Publishing; 2012.
Jackson BP, Snowdon DW. Atlas of microscopy of medicinal plants, culinary herbs and spices. London: Belhaven press; 1990.
Mozaffarian V. A Dictionary of Iranian Plant Names. Tehran: Farhang Moaser Publishers; 1996.
Rechinger KH. Flora Iranica. Vol. 150. Austria: Akademische Druck-U. Verlagsanstalt; 1982.
Couldis M, Tanimanidis A, Tzakou O, Chinou IB, Harvala C. Essential oil of Phlomis lanata growing in Greece: chemical composition and antimicrobial activity. Planta Med 2000;66:670-2.
Kirmizibekmez H, Montoro P, Piacente S, Pizza C, Doenmez A, Calis I. Identification by HPLC-PAD-MS and quantification by HPLC-PAD of phenylethanoid glycosides of five Phlomis species. Phytochem Anal 2005;16:1-6.
Saracoglu I, Kojima K, Harput US, Ogihara Y. A new phenylethanoid glycoside from Phlomis pungens Willd. var. pungens. Chem Pharm Bull 1998;46:726-7.
Sarkhail P, Abdollahi M, Shafiee A. Antinociceptive effect of Phlomis olivieri Benth., Phlomis anisodonta Boiss. and Phlomis persica Boiss. total extracts. Pharmacol Res 2003;48:263-6.
Sarkhail P, Abdollahi M, Fadayevatan S, Shafiee A, Mohammadirad A, Dehghan G, et al. Effect of Phlomis persica on glucose levels and hepatic enzymatic antioxidants in streptozotocin-induced diabetic rats. Pharmacogn Mag 2010;6:219-22.
Kamel MS, Mohamed KM, Hassanean HA, Ohtani K, Kasai R, Yamasaki K. Iridoid and megastigmane glycosides from Phlomis aurea. Phytochemistry 2000;55:353-7.
Katagiri M, Ohtani K, Kasai R, Yamasaki K, Yang CR, Tanaka O. Diterpenoid glycosyl esters from Phlomis younghusbandii and P. medicinalis roots. Phytochemistry 1994;32:439-42.
Kirmizibekmez H, Calis I, Perozzo R, Brun R, Doenmez AA, Linden A, et al. Inhibiting activities of the secondary metabolites of Phlomis brunneogaleata against parasitic protozoa and plasmodial enoyl-ACP Reductase, a crucial enzyme in fatty biosynthesis. Planta Med 2004;70:711-7.
Kyriakopoulo I, Magiatis P, Skaltounis AL, Aligiannis N, Samioside HC. A new phenylethanoid glycoside with free-radical scavenging and antimicrobial activities from Phlomis samia. J Nat Prod 2001;64:1095-7.
Shin TY, Lee JK. Effect of Phlomis umbrosa root on mast cell-dependent immediate-type allergic reactions by anal therapy. Immunopharmacol Immunotoxicol 2003;25:73-85.
Dellai A, Mansour HB, Limem I, Bouhlel I, Sghaier MB, Boubaker J, et al. Screening of antimutagenicity via antioxidant activity in different extracts from the flowers of Phlomis crinita Cav. Ssp Mauritania munby from the center of Tunisia. Drug Chem Toxicol 2009;32:283-92.
Kumar R, Bhan S, Kalla AK, Dhar KL. Flavonol glycosides of Phlomis spectabilis. Phytochemistry 1985;24:1124-5.
Saracoglu I, Varel M, Calis I. Neolignan, flavonoid, phenylethanoid and iridoid glycosides from Phlomis integrifolia. Turk J Chem 2003;27:739-47.
Manayi A, Mirnezami T, Saeidnia S, Ajani Y. Pharmacognostical evaluation, phytochemical analysis and antioxidant activity of the roots of Achillea tenuifolia. Pharmacogn J 2012;4:14-9.
Manayi A, Khanavi M, Saeidnia S, Azizi E, Mahmoodpour MR, Vafi F, et al. Biological activity and microscopic characterization of Lythrum salicaria L. Daru J Pharm Sci 2013;21:1-7.
Vazirian M, Dianat S, Manayi A, Ziari R, Mousazadeh A, Habibi E, et al. Anti-inflammatory effect, total polysaccharide, total phenolics content and antioxidant activity of the aqueous extract of three basidiomycetes. Res J Pharmacogn 2014;1:13-9.
Yumrutas O, Saygideger SD. Determination of antioxidant and antimutagenic activities of Phlomis armeniaca and Mentha pulegium. J. Appl Pharm Sci 2012;2:36-40.
Tannin-Spitz T, Bergman M, van-Moppes D, Grossman S, Arad S. Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp. J Appl Phycol 2005;17:215-22.
Tsiapali E, Whaley S, Kalbfleisch J, Ensley HE, Browder IW, Williams DL. Glucans exhibit weak antioxidant activity, but stimulate macrophage free radical activity. Free Radical Biol Med 2001;30:393-402.
Zhang Y, Wang ZZ. Phenolic composition and antioxidant activities of two Phlomis species: A correlation study. C R Biol 2009;332:816-26.
López V, Jäger AK, Akerreta S, Cavero RY, Calvo MI. Antioxidant activity and phenylpropanoids of Phlomis lychnitis L.: a traditional herbal tea. Plant Foods Hum Nutr 2010;65:179-85.
Sarkhail P, Nikan M, Sarkheil P, Gohari AR, Ajani Y, Hadjiakhoondi A, et al. Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships. Daru J Pharm Sci 2014;22:2-9.