UV SPECTROPHOTOMETRIC ESTIMATION OF SUNITINIB MALATE IN PHARMACEUTICAL DOSAGE FORM
Keywords:
Sunitinib malate, UV Spectroscopy, D0, D1 and D2 order derivative modesAbstract
Objective: To develop and validate simple zero (D0), first (D1) and second (D2) order derivative UV Spectrophotometric methods for the determination of Sunitinib malate in pharmaceutical dosage form.
Methods: Sunitinib malate was solubilised in distilled water and the resultant solution exhibits adsorption maximum (λmax) at 431, 457 and 489 nm in D0, D1 and D2 order derivative modes respectively. The developed method was validated as per ICH guidelines [1].
Results: Linearity was obtained over the concentration range of 2-12 µg/ml in all the derivative modes. Limit of detection (LOD) was found to be 0.291, 0.107, 0.327μg/ml and Limit of quantification (LOQ) was found to be 0.883, 0.324, 0.993μg/ml for D0, D1 and D2 order derivative modes respectively. The proposed method demonstrated an excellent intra-day precision and inter-day precision. Mean recovery was found within the range of 98.19-98.62% respectively, signifies the accuracy of the developed method.
Conclusion: The statistical results prove that the developed method can be effectively applied for the routine analysis of Sunitinib malate in industries and other analytical laboratories.
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References
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