ASSESSMENT OF GENETIC DIVERSITY OF TRIGONELLA FOENUM-GRACEUM L. IN NORTHERN INDIA USING RAPD AND ISSR MARKERS
Keywords:
Genetic diversity, ISSR, Polymorphism, RAPD, Trigonella foenum-graecum LAbstract
Objective: The main aim of this study was to assess genetic diversity and phylogenetic relationships of different varieties of fenugreek (8 varieties and 6 populations) collected from northern India using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter Simple Sequence Repeat markers).
Methods: DNA Samples were analyzed with 400 RAPD and 100 ISSR primers. For statistical analysis data were subjected to UPGMA (Un weighted pair-group method with arithmetic averages), Jaccard's similarity coefficient values were obtained using Similarity for Qualitative Data (SIMQUAL), to find out genetic relationship. This analysis generated dendrogram which was further compared using the Mantel matrix correspondence test (NTSyS-pc version 2.02i) and Principal coordinates analysis.
Results: Polymorphism frequency of 42.91% and 55.66% was found in different varieties of fenugreek using RAPD and ISSR markers respectively. Based on the combined data of RAPD+ISSR marker system the maximum similarity index was observed for accessions from Mathura and RMT-143 (0.85) whereas the minimum similarity index was observed for RMT-351 sand RMT-303 (0.26).
Conclusion: The investigation has demonstrated that cluster analysis could be profitably used in unravelling the genetic variation within the accessions and the two molecular markers, RAPD and ISSR could be used as effective tools to evaluate genetic diversity and assess genetic relationship in fenugreek.
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