EXTRACTION, PURIFICATION AND PHYSICOCHEMICAL EVALUATION OF MUCILAGE OF CHRYSOPHYLLUM LANCEOLATUM (BLUME) DC FRUITS: AN INVESTIGATION FOR BIOADHESIVE PROPERTY
Abstract
Objective: The objective of the present investigation is to extract, purify and characterize the physicochemical properties of the mucilage obtained from the fruits of Chrysophyllum lanceolatum (Blume) DC and also to evaluate the bioadhesiveness of Chrysophyllum lanceolatum Mucilage (CLM) to be used as the biodegradable mucoadhesive agent.
Methods: The mucilage was extracted, purified and identified by the methods as described in the official books. Purified CLM was characterized for physicochemical properties, swelling capacity, loss on drying and flow properties. The CLM was further characterized by Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC) and High-Performance Liquid Chromatography (HPLC). The mucoadhesivity of the mucilage was assessed by shear stress method and falling sphere method using goat intestine as mucosal substrate and the results were compared with Hydroxy Propyl Methyl Cellulose (HPMC) and Carbopol 934P (CP). The acute oral toxicity study was also conducted in Swiss Albino mice.
Results: The nature of the CLM is confirmed as polysaccharide from the experimental data. The physicochemical property and toxicity study also shows its acceptability as bioadhesive excipient. The bioadhesive test of CLM showed a good adhesive strength to the biological membrane.
Conclusion: The present investigation showed better bioadhesive property of the isolated mucilage and hence may provide an alternative to conventional synthetic/semi-synthetic mucoadhesive agents.
Keywords: Chrysophyllum lanceolatum, Mucilage, Extraction, Physicochemical, Bioadhesivity, Mucosal.
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