ANTIDIABETIC STUDY OF COMBINATION OF ANDROGRAPHIS PANICULATA (BURM. F.) WALLICH. EX NEES. HERBS EXTRACT AND GUAZUMA ULMIFOLIA LAMK. LEAVES EXTRACT IN OBESE DIABETIC MICE MODEL
Keywords:
Diabetes mellitus, Obesity, Andrographis paniculata, Guazuma ulmifolia, MiceAbstract
Objective: This research was to investigate the activity of Andrographis paniculata extract (APE) and Guazuma ulmifolia extract (GUE) in treating obesity integrated diabetic conditions.
Methods: There were two stages of inductions: the first stage was a 42 d of high carbohydrate intake (obese with insulin resistance mice model), followed by second stage induction by giving of alloxan 50 mg/kg bw intravenously (obese diabetic mice model). Animals with glucose level>200 mg/dl were then treated with metformin 195 mg/kg bw, glibenclamide 0.65 mg/kg bw, APE 2 g/kg bw, GUE 0.5 g/kg bw, combination of APE 1 g/kg bw and GUE 0.25 g/kg bw, or combination of APE 2 g/kg bw and GUE 0.5 g/kg bw for 14 d.
Results: The results showed that APE alone, GUE alone, and the combination of APE 2 g/kg bw and GUE 0.5 g/kg bw could improve insulin sensitivity in obese with insulin resistance mice model. Whereas the combination of APE 2 g/kg bw and GUE 0.5 g/kg bw could significantly decrease blood glucose level and body weight (p<0.05) in obese diabetic mice model compare to APE alone, GUE alone, or a combination of APE 1 g/kg bw and GUE 0.25 g/kg bw.
Conclusion: It is concluded that APE 2 g/kg bw, GUE 0.5 g/kg bw, and combination of APE 2 g/kg bw and GUE 0.5 g/kg bw can improve insulin resistance conditions caused by obesity, while combination of APE 2 g/kg bw and GUE 0.5 g/kg bw has the best activity in treating obese diabetic conditions.
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