EVALUATION OF INVITRO ANTIOXIDANT POTENTIAL OF THE TOTAL CRUDE ALKALOID EXTRACT OF GLYCOSMIS PENTAPHYLLA (RETZ.) CORREA LEAVES
Abstract
Objective: The present investigation was undertaken to appraise the antioxidant properties and the total crude alkaloid extract of Glycosmis pentaphylla leaves.
Methods: Radical attenuating abilities of total crude alkaloid extract were ascertained by six in vitro assays such as 2, 2-diphenyl 1-picryl hydrazyl (DPPH·) radical scavenging assay, ferric reducing antioxidant power assay (FRAP), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radical scavenging assay, nitric oxide (NO) scavenging assay, hydroxyl radical (OH·) scavenging assay, and reducing power capacity assessment.
Results: The total crude alkaloid extract showed a considerable antioxidant potentiality in comparison with the standards as evidenced by the IC50 values obtained in the DPPH· (966.93 µg/ml), FRAP (510.81 µg/ml), ABTS•+(400.47 µg/ml), OH· (1805.28 µg/ml), NO (1426.50 µg/ml). In the reducing power assay, the total crude alkaloid extract showed a concentration dependent reducing power potential.
Conclusion: These results suggest the potential of Glycosmis pentaphylla as a curative against free-radical-associated oxidative damage diseases.
Keywords: Glycosmis pentaphylla, Antioxidant assay, Total crude alkaloid, IC50
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References
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