EFFECT OF COPPER ON THE GENERATION TIME AND ANTIOXIDANT POTENTIAL OF A NOVEL ISOLATE OF CHLORELLA EMERSONII KJ725233
Abstract
Objective: At low concentrations copper is an essential micronutrient for algal growth wherein it plays a vital role as an enzyme cofactor for photosynthetic processes but at high concentrations it functions as a toxic heavy metal. Copper exposure to microalga increases the activity of antioxidative enzymes–catalase, superoxide dismutase, peroxidase along with the induction of chlorosis at higher concentrations. The objective of the present study is to determine the effect of suboptimal concentration of copper on the generation time as well as the antioxidant potential of a novel strain-Chlorella emersonii KJ725233.
Methods: The growth of the microalga at the different copper concentrations was monitored by measuring the absorbance at 684 nm. Once the suboptimum copper concentration for the growth of the microalga was determined, the methanolic extracts were evaluated for Total Phenolic Content, Total Flavonoid Contents as well as the antioxidant potential by employing standard methods such as phospho- molybdenum method for Total Antioxidant Capacity and Ferric Reducing Antioxidant Potential.
Results: Results indicated that 0.1 µmol of copper stimulated the growth of Chlorella emersonii KJ725233, which was evident from the reduced generation time of the microalga as compared to that of the control and other copper concentrations. The study also proved a 45.69% increase in the antioxidant activity of the microalga on exposure to 0.1 µmol of copper.
Conclusion: The present study indicated copper at 0.1 µmol concentration not only acts as a micronutrient but also levies stress resulting in increased antioxidant activity of this novel isolate Chlorella emersonii KJ725233.
Keywords: Chlorella emersonii KJ725233, Copper, Generation time, Antioxidant
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References
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