• Ridha Aichour Laboratory of Applied Biochemistry, Faculty of Nature and Life Science, University Setif 1, Algeria
  • Noureddine Charef University Setif
  • Abderrahmane Baghiani University Setif
  • Lekhmici Arrar University Setif


Capparis spinosa, Immunomodulatory properties, Hypersensitivity, Humoral response, Lymphocyte proliferation


Objective: The aim of this study is to investigate the immunomodulatory properties of fruits and leaves of Capparis spinosa in mice.

Methods: The effect of methanolic extracts of C. spinosa on immune system were assessed by applying several approaches such as Lymphocyte proliferation assay in presence of mitogen (Concanavalin A), delayed-type hypersensitivity (DTH) response, humoral response and Cyclophosphamide-induced immunosuppression.

Results: Administration of methanolic extracts at doses 100 and 200 mg/kg produced statistically significant results as evidenced by the increase in delayed type hypersensitivity (DTH) response (P<0.05), enhanced the total WBC level in the cyclophosphamide induced myelosuppression model (p<0.01). These extracts also showed significant increase in humoral antibody (HA) titre (P<0.05, P<0.01) at dose 200 mg/ml. Equally, C. spinosa extracts evoked a significant (p<0.05, P<0.01) increase in mitogen-induced lymphocyte proliferation.

Conclusions: The results demonstrated that both the plant parts extracts exert a marked immunostimulatory effect on the mouse immune system.


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Sharma P, Charaka Samhita, Chikitsasthana. Chaukhamba Orientalia, Varanasi; 1983. p. 2.

Wagner H. Economic and medicinal plant research. Vol. 1. Hikino H, Farnsworth NR. Academic Press: London; 1984. pp. 113–53.

Tlili N, Elfalleh W, Saadaoui E, Khaldi A, Triki S, Nasri N. The caper (Capparis L.): Ethnopharmacology, phytochemical and pharmacological properties. Fitoterapia 2011;82:93–101.

Jouad H, Haloui M, Rhiouani H, El Hilaly J, Eddouks M. Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez-Boulemane). J Ethnopharmacol 2011;77:175–82.

Eddouks M, Maghrani M, Lemhadri A, Ouahidi ML, Jouad H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the southeast region of morocco (Tafilalet). J Ethnopharmacol 2002;82:97–103.

Kirtikar KR, Basu BD. Indian medicinal plants periodical. Expert Book Agency, Delhi; 1991. p. 197–8.

Kanasea VG, Jain BB, Yadav P. Evaluation of in-vitro immunomodulatory activity of an aqueous and ethanolic extract of Capparis moonii. Int J Pharma Bio Sci 2013;4:344–52.

Arena A, Bisignano G, Pavone B, Tomaino A, Bonina FP, Saija A, et al. Antiviral and immunomodulatory effect of a lyophilized extract of Capparis spinosa L. buds. Phytother Res 2008;22:313–7.

Arrar L, Benzidane N, Krache I, Charef N, Khennouf S, Baghiani A. Comparison between polyphenol contents and antioxidant activities of different parts of Capparis spinosa L. Pharmacogn Commun 2013;3:70-4.

Alsever JB, Ainslie RB. A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. N Y State J Med 1941;41:126-31.

Amirghofran Z, Bahmani M, Azadmehr A, Javidnia K, Ramazani M, Ziaei A. Effect of Salvia mirzayanii on the immune system and induction of apoptosis in peripheral blood lymphocytes. Nat Prod Res 2010;24:500-8.

Doherty NS. Selective effect of immunosuppressive agents on the delayed hypersensitivity response and humoral response to sheep red blood cells in mice. Agents Actions 1981;11:237-42.

Bafna AR, Mishra SH. Immunomodulatory activity of methanol extract of flower-heads of Sphaeranthus indicus Linn. Ars Pharm 2004;45:281-91.

Potjo M, Cockeran R, Theron AJ, Feldman C, Anderson R. Effects of moxifloxacin on human neutrophil and T-lymphocyte functions in vitro. Pharmaceuticals 2010;3:3570-80.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J Immunol Methods 1983;65:55-63.

Neha J, Mishra RN. Immunomodulatory activity of Trikatu mega ext. Int J Res Pharm Biomed Sci 2011;2:160-4.

Miller LE, Ludke HR, Peacock JE, Tomar RH. Manual of Laboratory Immunology. Lea and Febeiger, London; 1991. p. 1-18.

Benacerraf B. A hypothesis to relate the specificity of T lymphocytes and the activity of I region specific Ir genes in macrophages and B lymphocytes. J Immunol 1978;120:1809-12.

Wood P. Understanding Immunology. Benjamin Cummings. 2 ed. England; 2006. p. 294.

Owen JA, Punt J, Stranford SA. Kuby immunology. Freeman WH, New York; 2013. p. 507.

Shah AS, Wakade AS, Juvekar AR. Immunomodulatory activity of methanolic extract of Murraya koenigii (L) Spreng. Leaves. Indian J Exp Biol 2008;46:505-9.

Mustafa FAA. In vitro evaluation of Capparis spinosa against Lumbricus terrestris (Annelida). Pontif Univ Javeriana 2012;5:199-202.

Harsha N, Sridevi V, Chandana Lakshmi MVV, Rani K, Vani NDS. Phytochemical analysis of some selected spices. Int J Innovative Res Sci Eng Technol 2013;2:6618-21.

Kawakita SW, Griedlin HS, Nomoto K. Immunomodulators from higher plants. J Nat Med 2005;46:34-8.

Moghaddasian B, Eradatmand AD, Alaghemand A. Simultaneous determination of rutin and quercetin in different parts of Capparis spinosa. Bull Environ Pharmacol Life Sci 2013;2:35-8.



How to Cite

Aichour, R., N. Charef, A. Baghiani, and L. Arrar. “IMMUNOMODULATORY EFFECTS OF ALGERIAN CAPER”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 2, Feb. 2016, pp. 51-54,



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